Recombinant Human One cut domain family member 2 (ONECUT2)

Code CSB-YP016337HU
MSDS
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Source Yeast
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Code CSB-EP016337HU
MSDS
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Source E.coli
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Code CSB-EP016337HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP016337HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP016337HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ONECUT2
Uniprot No.
Alternative Names
ONECUT2; HNF6B; One cut domain family member 2; Hepatocyte nuclear factor 6-beta; HNF-6-beta; One cut homeobox 2; Transcription factor ONECUT-2; OC-2
Species
Homo sapiens (Human)
Expression Region
1-504
Target Protein Sequence
MKAAYTAYRC LTKDLEGCAM NPELTMESLG TLHGPAGGGS GGGGGGGGGG GGGGPGHEQE LLASPSPHHA GRGAAGSLRG PPPPPTAHQE LGTAAAAAAA ASRSAMVTSM ASILDGGDYR PELSIPLHHA MSMSCDSSPP GMGMSNTYTT LTPLQPLPPI STVSDKFHHP HPHHHPHHHH HHHHQRLSGN VSGSFTLMRD ERGLPAMNNL YSPYKEMPGM SQSLSPLAAT PLGNGLGGLH NAQQSLPNYG PPGHDKMLSP NFDAHHTAML TRGEQHLSRG LGTPPAAMMS HLNGLHHPGH TQSHGPVLAP SRERPPSSSS GSQVATSGQL EEINTKEVAQ RITAELKRYS IPQAIFAQRV LCRSQGTLSD LLRNPKPWSK LKSGRETFRR MWKWLQEPEF QRMSALRLAA CKRKEQEPNK DRNNSQKKSR LVFTDLQRRT LFAIFKENKR PSKEMQITIS QQLGLELTTV SNFFMNARRR SLEKWQDDLS TGGSSSTSST CTKA
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcriptional activator. Activates the transcription of a number of liver genes such as HNF3B.
Gene References into Functions
  1. Bioinformatics-based prediction, combined with qRT-PCR analysis and Western blotting, identified Onecut2 and SIRT1 as the 2 prominent miR-9 target genes under the conditions tested. In this study, Onecut2 and SIRT1 were confirmed as miR-9 target genes, which will help future investigations to probe in greater detail the role of miR-9 in Nonalcoholic Fatty Liver Disease. PMID: 27756894
  2. Data show that ONECUT2, IGF2BP1, and ANXA2 proteins were confirmed to be microRNA-9 (miR-9) targets and aberrantly upregulated in hepatocellular carcinoma (HCC). PMID: 26547929
  3. our data showed that transcript factor Onecut2 is involved in the EMT, migration and invasion of CRC cells; miR-429 inhibits the initiation of EMT and regulated expression of EMT-related markers PMID: 24402783
  4. Mutations at the -5/-6 site of the F9 promotor account for the majority of hemophilia B Leyden cases and disrupt the binding of ONECUT1 and ONECUT2. PMID: 23472758
  5. OC2 transcription factor is a direct target of T-bet in type-1 T-helper cells. PMID: 18418398
  6. A CpG island proximal to ONECUT2 is frequently methylated in diffuse large B-cell lymphoma (DLBCL). PMID: 18288132

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Subcellular Location
Nucleus.
Protein Families
CUT homeobox family
Database Links

HGNC: 8139

OMIM: 604894

KEGG: hsa:9480

STRING: 9606.ENSP00000262095

UniGene: Hs.194725

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