ONECUT2 Antibody

Code CSB-PA016337LA01HU
Size US$166
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  • Western Blot
    Positive WB detected in: A549 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate
    All lanes: ONECUT2 antibody at 2.4µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 55 kDa
    Observed band size: 55 kDa

  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA016337LA01HU at dilution of 1:100

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) ONECUT2 Polyclonal antibody
Uniprot No.
Target Names
ONECUT2
Alternative Names
ONECUT2 antibody; HNF6B antibody; One cut domain family member 2 antibody; Hepatocyte nuclear factor 6-beta antibody; HNF-6-beta antibody; One cut homeobox 2 antibody; Transcription factor ONECUT-2 antibody; OC-2 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human One cut domain family member 2 protein (214-331AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The ONECUT2 Antibody (Product code: CSB-PA016337LA01HU) is Non-conjugated. For ONECUT2 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA016337LB01HU ONECUT2 Antibody, HRP conjugated ELISA
FITC CSB-PA016337LC01HU ONECUT2 Antibody, FITC conjugated
Biotin CSB-PA016337LD01HU ONECUT2 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

ONECUT2 antibody CSB-PA016337LA01HU was produced in the rabbits immunized by using the recombinant human ONECUT2 protein (214-331aa) as the immunogen. The target protein ONECUT2 mainly regulates gene expression in various tissues, playing a crucial role in the development, differentiation, and cellular processes critical for proper physiological function. Aberrant expression or activity of ONECUT2 has been implicated in various diseases, including diabetes, liver disease, and neurodevelopmental disorders.

This rabbit anti-human ONECUT2 polyclonal antibody was quality tested in the ELISA, WB, and IHC applications. The non-conjugated IgG got purified by protein G and reached up to 95% in purity. It only reacts with the ONECUT2 proteins from human samples.

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Target Background

Function
Transcriptional activator. Activates the transcription of a number of liver genes such as HNF3B.
Gene References into Functions
  1. Bioinformatics-based prediction, combined with qRT-PCR analysis and Western blotting, identified Onecut2 and SIRT1 as the 2 prominent miR-9 target genes under the conditions tested. In this study, Onecut2 and SIRT1 were confirmed as miR-9 target genes, which will help future investigations to probe in greater detail the role of miR-9 in Nonalcoholic Fatty Liver Disease. PMID: 27756894
  2. Data show that ONECUT2, IGF2BP1, and ANXA2 proteins were confirmed to be microRNA-9 (miR-9) targets and aberrantly upregulated in hepatocellular carcinoma (HCC). PMID: 26547929
  3. our data showed that transcript factor Onecut2 is involved in the EMT, migration and invasion of CRC cells; miR-429 inhibits the initiation of EMT and regulated expression of EMT-related markers PMID: 24402783
  4. Mutations at the -5/-6 site of the F9 promotor account for the majority of hemophilia B Leyden cases and disrupt the binding of ONECUT1 and ONECUT2. PMID: 23472758
  5. OC2 transcription factor is a direct target of T-bet in type-1 T-helper cells. PMID: 18418398
  6. A CpG island proximal to ONECUT2 is frequently methylated in diffuse large B-cell lymphoma (DLBCL). PMID: 18288132

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Subcellular Location
Nucleus.
Protein Families
CUT homeobox family
Database Links

HGNC: 8139

OMIM: 604894

KEGG: hsa:9480

STRING: 9606.ENSP00000262095

UniGene: Hs.194725

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