Recombinant Human Organic solute transporter subunit beta (OSTB), partial

Code CSB-YP771440HU
MSDS
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Source Yeast
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Code CSB-EP771440HU
MSDS
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Source E.coli
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Code CSB-EP771440HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP771440HU
MSDS
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Source Baculovirus
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Code CSB-MP771440HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SLC51B
Uniprot No.
Alternative Names
SLC51B; OSTB; Organic solute transporter subunit beta; OST-beta; Solute carrier family 51 subunit beta
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Essential component of the Ost-alpha/Ost-beta complex, a heterodimer that acts as the intestinal basolateral transporter responsible for bile acid export from enterocytes into portal blood. Efficiently transports the major species of bile acids. Modulates SLC51A glycosylation, membrane trafficking and stability activities.
Gene References into Functions
  1. Dileucine motif in the extracellular N-terminal region is essential for OSTB plasma membrane targeting. PMID: 27351185
  2. Hepatic OSTalpha-OSTbeta expression is induced by hypoxia. PMID: 24703425
  3. OSTbeta is a target of RARalpha-mediated (by binding to DR5 response element) gene regulation pathways PMID: 24264050
  4. Ostbeta is required for both proper trafficking of Ostalpha and formation of the functional transport unit, and identify specific residues of Ostbeta critical for these processes. PMID: 22535958
  5. The present report summarizes the evidence for a pleiotropic role of Ostalpha-Ostbeta in different tissues. PMID: 21691099
  6. OSTbeta is localized to steroidogenic cells of the brain and adrenal gland, and it modulates DHEA/DHEAS homeostasis PMID: 20649839
  7. OSTbeta has roles in biological transport and is widely expressed in human tissues PMID: 12719432
  8. overexpression of human OSTalpha and OSTbeta facilitated the uptake of conjugated chenodeoxycholate and the activation of FXR target genes PMID: 16251721
  9. OSTalpha/OSTbeta expression is induced by bile acids through ligand-dependent transactivation of both OST genes by the nuclear bile acid receptor/farnesoid X receptor (FXR). PMID: 16269519
  10. the selective localization of OSTalpha and OSTbeta to the basolateral plasma membrane of epithelial cells responsible for bile acid and sterol reabsorption. PMID: 16317684
  11. These results indicate that expression of Ostalpha and Ostbeta are highly regulated in response to cholestasis and that this response is dependent on the FXR bile acid receptor. PMID: 16423920
  12. Demonstrate association of OST-alpha and OST-beta to determine trafficking to plasma membrane and activity. PMID: 17332473
  13. The mRNA expression of OSTalpha-OSTbeta was significantly reduced (OSTalpha: 3.3-fold, P = 0.006; OSTbeta: 2.6-fold, P = 0.03) in normal-weight but not overweight gallstone carriers PMID: 18469300
  14. interaction of solute transporter beta with human organic solute transporter alpha. PMID: 18847488

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Subcellular Location
Cell membrane; Single-pass membrane protein. Note=Mainly restricted to the lateral and basal membranes of ileal enterocytes.
Protein Families
OST-beta family
Tissue Specificity
Widely expressed with a high expression in ileum. Expressed in testis, colon, liver, small intestine, kidney, ovary and adrenal gland; and at low levels in heart, lung, brain, pituitary, thyroid gland, uterus, prostate, mammary gland and fat.
Database Links

HGNC: 29956

OMIM: 612085

KEGG: hsa:123264

STRING: 9606.ENSP00000335292

UniGene: Hs.27018

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