Recombinant Human PHD finger protein 20 (PHF20), partial

Code CSB-YP017909HU
MSDS
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Source Yeast
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Code CSB-EP017909HU
MSDS
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Source E.coli
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Code CSB-EP017909HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP017909HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP017909HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PHF20
Uniprot No.
Alternative Names
C20orf104; GLEA2; Glioma-expressed antigen 2; HCA58; Hepatocellular carcinoma-associated antigen 58; Novel zinc finger protein; NZF; PHD finger protein 20; PHF20; PHF20_HUMAN; Transcription factor TZP; TZP
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Methyllysine-binding protein, component of the MOF histone acetyltransferase protein complex. Not required for maintaining the global histone H4 'Lys-16' acetylation (H4K16ac) levels or locus specific histone acetylation, but instead works downstream in transcriptional regulation of MOF target genes. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. Contributes to methyllysine-dependent p53/TP53 stabilization and up-regulation after DNA damage.
Gene References into Functions
  1. These findings establish a unique PHF20-mediated link between MOF histone acetyltransferase (HAT), p53, and dimethylated lysine 4 of histone H3, and suggest a model for rapid spreading of H4K16ac-enriched open chromatin. PMID: 27760318
  2. PHF20 may play an important role in Non-small-cell lung cancer (NSCLC). PMID: 26722404
  3. suggests a del(20q)-independent decrease in PHF20 expression in primary myelofibrosis PMID: 24675105
  4. Plant homeodomain finger protein 20 (PHF20) maintains NF-kappaB in an active state in the nucleus by inhibiting the interaction between PP2A and p65. PMID: 23797602
  5. PHF20 is a novel substrate for PKB and its phosphorylation by PKB plays an important role in tumorigenesis via regulating of p53 mediated signaling. PMID: 22975685
  6. Association with PHF20 promotes stabilization and activation of p53 by diminishing Mdm2-mediated p53 ubiquitylation and degradation. PMID: 22864287
  7. Data report the crystal structures of the N-terminal Tudor domains of PHF20 and highlight the novel structural features of each domain. PMID: 22449972
  8. genetic alterations of TSPAN14, SLC2A13 and PHF20 could play a role in non-small-cell lung cancer promotion PMID: 19473719
  9. GLEA2 seroreactivity was increased by the time of surgery, decreased after surgery, increased again under radiation, and slightly decreased after radiation PMID: 18478111

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Subcellular Location
Nucleus.
Tissue Specificity
Expressed in heart, kidney, liver, lung, pancreas, placenta, spleen and testis. Not expressed in brain, skeletal muscle, colon, ovary, prostate, small intestine and thymus. Expressed in colon and ovary cancer cell lines while it is not expressed in the re
Database Links

HGNC: 16098

OMIM: 610335

KEGG: hsa:51230

STRING: 9606.ENSP00000363124

UniGene: Hs.517044

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