Recombinant Human Peroxiredoxin-6(PRDX6)

Code CSB-YP018659HU
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Source Yeast
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Code CSB-EP018659HU
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Source E.coli
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Code CSB-EP018659HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP018659HU
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Source Baculovirus
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Code CSB-MP018659HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names PRDX6
Uniprot No. P30041
Alternative Names 1 Cys; 1 Cys peroxiredoxin; 1 Cys PRX; 1 cysPrx; 1-Cys peroxiredoxin; 1-Cys PRX; 24 kDa protein; 9430088D19Rik ; AA690119; Acidic calcium independent phospholipase A2 ; Acidic calcium-independent phospholipase A2; aiPLA2; Antioxidant protein 2; AOP2; Aop2 rs3; Brp 12; Ciliary body glutathione peroxidase ; CP 3; EC 1.11.1.15; EC 1.11.1.7 ; EC 3.1.1.; Epididymis secretory sperm binding protein Li 128m; GPx ; HEL S 128m; KIAA0106; Liver 2D page spot 40; Ltw4; Lvtw 4; MGC46173; mKIAA0106; Non selenium glutathione peroxidase; Non-selenium glutathione peroxidase; NSGPx; ORF06; OTTHUMP00000032693 ; p29; Peroxiredoxin-6; Peroxiredoxin6; PHGPx; Phospholipase A2 lysosomal ; PLA2; PRDX 6; Prdx5; PRDX6; Prdx6 rs3; PRDX6_HUMAN; PRX; Red blood cells page spot 12; Thiol specific antioxidant protein
Species Homo sapiens (Human)
Expression Region 2-224
Target Protein Sequence PGGLLLGDV APNFEANTTV GRIRFHDFLG DSWGILFSHP RDFTPVCTTE LGRAAKLAPE FAKRNVKLIA LSIDSVEDHL AWSKDINAYN CEEPTEKLPF PIIDDRNREL AILLGMLDPA EKDEKGMPVT ARVVFVFGPD KKLKLSILYP ATTGRNFDEI LRVVISLQLT AEKRVATPVD WKDGDSVMVL PTIPEEEAKK LFPKGVFTKE LPSGKKYLRY TPQP
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. Also has phospholipase activity, can therefore either reduce the oxidized sn-2 fatty acyl group of phospholipids (peroxidase activity) or hydrolyze the sn-2 ester bond of phospholipids (phospholipase activity). These activities are dependent on binding to phospholipids at acidic pH and to oxidized phospholipds at cytosolic pH. Plays a role in cell protection against oxidative stress by detoxifying peroxides and in phospholipid homeostasis. Exhibits acyl-CoA-dependent lysophospholipid acyltransferase which mediates the conversion of lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine or LPC) into phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC). Shows a clear preference for LPC as the lysophospholipid and for palmitoyl CoA as the fatty acyl substrate.
Gene References into Functions
  1. These findings implicate miR-371-3p as a suppressor of PRDX6 and suggest that co-targeting of peroxiredoxin 6 or modulating miR-371-3p expression together with targeted cancer therapies may delay or prevent acquired drug resistance. PMID: 27484502
  2. The present data suggest that PS2 mutations suppress lung tumor development by inhibiting the iPLA2 activity of PRDX6 via a gamma-secretase cleavage mechanism and may explain the inverse relationship between lung cancer and Alzheimer's disease incidence. PMID: 29109765
  3. The increased HDL and plasma levels of PRDX6 in AAA patients support the potential of PRDX6 as a new biomarker of AAA. PMID: 27934969
  4. These observations demonstrated that the expression and localization of NPM affected the homeostatic balance of oxidative stress in tumor cells via PRDX6 protein. The regulation axis of NPM/PRDX/ROS may provide a novel therapeutic target for cancer treatment. PMID: 28513872
  5. the transition-state substrate analogue inhibitor of Prdx6 phospholipase A2 activity (MJ-33) was shown to suppress Nox1 activity, suggesting Nox1 activity is regulated by the phospholipase activity of Prdx6. Finally, wild type Prdx6, but not lipase or peroxidase mutant forms, supports Nox1-mediated cell migration in the HCT-116 colon epithelial cell model of wound closure PMID: 27094494
  6. The constitutive elevations of Prdx6 and NF-kappaB during Clonorchis sinensis infection may be associated with more severe persistent hepatobiliary abnormalities mediated by clonorchiasis. PMID: 27554973
  7. Peroxidase activity was markedly reduced by mutation at either of the Leu sites and was essentially abolished by the double mutation, while PLA2 activity was unaffected. Decreased peroxidase activity following mutation of the interfacial leucines presumably is mediated via impaired heterodimerization of Prdx6 with piGST that is required for reduction and re-activation of the oxidized enzyme. PMID: 26891882
  8. PRX6 protein is associated with fetal esophageal development and cancer differentiation. PMID: 28293090
  9. PRDX6 inhibited the carcinogenesis of hepatocellular carcinoma, and the calcium-independent phospholipase A2 activity of PRDX6 promoted cancer cell death induced by TNF-alpha. PMID: 26293541
  10. the data demonstrate that Prdx6 interrupts the formation of TRAF6-ECSIT complex induced by TLR4 stimulation, leading to suppression of bactericidal activity because of inhibited mROS production in mitochondria and the inhibition of NF-kappaB activation in the cytoplasm. PMID: 28393051
  11. Thus far only the crystal structure of Prx in the oxidized state has been reported. In this study, we present the crystal structures of human Prx6 in the reduced (SH) and the sulfinic acid (SO2H) forms. PMID: 27353378
  12. Peroxiredoxin 6 has a role in the repair of peroxidized cell membranes and cell signaling [review] PMID: 27932289
  13. PRDX6-anion exchanger 1 interaction contributes to the maintenance of anion exchanger 1 during cellular stress such as during metabolic acidosis. PMID: 26398495
  14. Prdx6 is a complete enzyme comprising both PLA2and LPCAT activities PMID: 26830860
  15. Our study provides new insight into the initial regulatory mechanisms of mitophagy and reveals the protective role of PRDX6 in the clearance of damaged mitochondria. PMID: 26560306
  16. Thr can substitute for Ser for the enzymatic activities of Prdx6 but not for its targeting to LB. These results confirm an important role for LB Prdx6 in the degradation and remodeling of lung surfactant phosphatidylcholine. PMID: 26921317
  17. Prdx6 and its PLA2 activity have a protective role in donors after brain death livers. PMID: 26647763
  18. PRDX6 may serve as a biomarker for traumatic brain injury and that autoimmune profiling is a viable strategy for the discovery of novel biomarkers PMID: 25938937
  19. PRDX6 protects ARPE-19 cells from hydrogen peroxide-induced oxidative stress and apoptosis. PMID: 26279427
  20. Delivery of a protein transduction domain-mediated Prdx6 protein protected against oxidative stress-evoked neuronal cell death. PMID: 26447207
  21. AA (arachidonic acid) as a crucial effector of PRDX6-dependent proliferation and inducer of Src family kinase activation. PMID: 26285655
  22. High intensity of cytoplasmic Prx VI expression in pretreatment diffuse large B-cell lymphoma samples predicts worse outcome. PMID: 25935550
  23. overexpression of PRDX6 promotes lung tumor growth via increased glutathione peroxidase and iPLA2 activities through the upregulation of the AP-1 and JNK pathways. PMID: 23643677
  24. Several regions of reduced PRDX6 are in a substantially different conformation from that of the crystal structure of the peroxidase catalytic intermediate. The differences between the two structures likely reflect catalysis-related conformational changes. PMID: 25748205
  25. our findings indicate that PRDX6 promotes lung tumor growth via increased glutathione peroxidase and iPLA2 activities. PMID: 24512906
  26. decreased levels, combined with higher thiol oxidation (and probable inhibition) of PRDXs (particularly PRDX6), are noted when human sperm function is altered. PMID: 22492841
  27. Reactive oxygen species-evoked aberrant sumoylation signaling affects Prdx6 activity by reducing Prdx6 abundance. PMID: 24910119
  28. Hypoexpression of PRDX6 is associated with papillary thyroid carcinomas. PMID: 24316730
  29. Apoptosis related proteins ERp29, PRDX6 and MPO were differentially expressed in placentas of pregnant women with intrahepatic cholestasis and in healthy pregnant women. PMID: 24391750
  30. KGF can activate an antioxidant response element in a promoter without reactive oxygen species involvement and that KGF and Dex can synergistically activate the PRDX6 promoter and protect cells from oxidative stress PMID: 23815338
  31. The level of Prx6 protein is lower in gastric cancer tissues than in normal para-cancer tissue. Prx6 expression is significantly correlated with the differentiation degree of GC. PMID: 23158669
  32. The protein and mRNA expressions of Prx1 and Prx6 increased significantly in the order of normal brain tissue, grade II astrocytoma, grade III astrocytoma and grade IV astrocytoma. PMID: 22985558
  33. Oxidation of the catalytic cysteine in Prdx6 is required for its interaction with PiGST, the interaction plays an important role in regenerating the peroxidase activity of Prdx6. PMID: 23164639
  34. Drug resistance formation was accompanied by a significant increase in the expression of PRDX1, PRDX2, PRDX3, PRDX6 genes in all cancer cell strains, which confirms the importance of redox-dependent mechanisms into development of cisplatin resistance. PMID: 23113308
  35. a change in the conformation of Prdx6 upon its phosphorylation is the basis for enhancement of PLA(2) enzymatic activity PMID: 22663767
  36. (2) activity of Prdx6-PLA(2) in intact cells mediates its ability to enhance phox activity in response to fMLF PMID: 22678913
  37. Data suggest that SNPs of peroxiredoxin 1, 2 and 6 are not associated with esophageal cancer. PMID: 22215146
  38. Data suggest that Prdx6 serves an additional biochemical or structural role in supporting optimal NADPH oxidase activity. PMID: 22178385
  39. Results provide new insights into the distinct roles of bifunctional Prdx6 with peroxidase and PLA(2) activities in oxidative stress-induced and TNF-induced apoptosis, respectively. PMID: 21415860
  40. expression and function of Prdx1 and Prdx6 in MCF-7 and noncancerous MCF-10A cell lines; found elevated Prdx1 expression in MCF-7 cells and comparable expression of Prdx6; data suggest synergistic role for Prdx1 and Prdx6 in MCF-10A cells PMID: 22236188
  41. Study revealed novel SNPs within the PRDX6 gene and its 5' and 3' flanking regions via direct sequencing. PMID: 21627785
  42. It could be detected that PRDX6 is associated with tumorigenesis in tonge squamous cell carcinoma. PMID: 20796224
  43. Prx6 modulates TRAIL signaling as a negative regulator of caspase-8 and caspase-10 in death-inducing signaling complex formation of TRAIL-resistant metastatic cancer cells. PMID: 20829884
  44. The antioxidant Prdx 6 protects ovarian cancer cells against cisplatin-induced apoptosis via inactivation of the caspase signaling pathway. PMID: 21166495
  45. This study is the first to define the functions of the enzymatic activities of PRDX6 in metastasis and to show the involvement of arachidonic acid in PRDX6 action in intact cells. PMID: 20354123
  46. promotes lung cancer cell invasion by inducing urokinase-type plasminogen activator via p38 kinase, phosphoinositide 3-kinase, and Ak PMID: 19937138
  47. Extrinsic PRDX6 prevents UV-triggered cell death and abnormal protein expression in PRDX6-deficient lens epitheial cells. PMID: 19889963
  48. Data show that p29 is a novel protein that associates with p67, has peroxiredoxin activity, and has a potential role in protecting the NADPH oxidase by inactivating H(2)O(2) or altering signaling pathways affected by H(2)O(2) [neutrophil protein p29 ] PMID: 12121978
  49. overexpression protects cells against phospholipid peroxidation-mediated membrane damage [1-cys peroxiredoxin] PMID: 12193653
  50. The significant increase in peroxiredoxin 6 level in frontal cortex of patients with Pick's disease is useful in discriminating it from Down syndrome/Alzheimer's disease. PMID: 12650976

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Subcellular Location Cytoplasm. Lysosome.
Protein Families Peroxiredoxin family, Prx6 subfamily
Database Links

HGNC: 16753

OMIM: 602316

KEGG: hsa:9588

STRING: 9606.ENSP00000342026

UniGene: Hs.120

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