Recombinant Human Phosphatidate phosphatase LPIN1(LPIN1) ,partial

Code CSB-YP614805HU
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Source Yeast
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Code CSB-EP614805HU
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Source E.coli
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Code CSB-EP614805HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP614805HU
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Source Baculovirus
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Code CSB-MP614805HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names LPIN1
Uniprot No. Q14693
Alternative Names EC=3.1.3.4; KIAA0188 ; Lipin-1; Lpin1; LPIN1_HUMAN; PAP1; Phosphatidate phosphatase LPIN1
Species Homo sapiens (Human)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function Plays important roles in controlling the metabolism of fatty acids at different levels. Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the reticulum endoplasmic membrane. Acts also as a nuclear transcriptional coactivator for PPARGC1A/PPARA to modulate lipid metabolism gene expression (By similarity). Is involved in adipocyte differentiation. May also be involved in mitochondrial fission by converting phosphatidic acid to diacylglycerol (By similarity).
Gene References into Functions
  1. Normal human adipose tissue functions and differentiation has been found in rhabdomyolysis patients with biallelic LPIN1 inactivating mutations. PMID: 28986436
  2. conclude that Lipin-1 can antagonize HSC activation through the inhibition of TGF-beta/SMAD signaling and that resveratrol may affect Lipin-1 gene induction and contribute to the inhibition of TGF-beta-mediated hepatic fibrogenesis PMID: 27345520
  3. beta-TRCP depletion in HepG2 hepatocellular carcinoma cells resulted in increased Lipin1 protein abundance. PMID: 28049764
  4. This study showed that LPIN1 knockdown blocks phospholipid synthesis and changes membrane lipid compositions, and that lipin-1 knockdown significantly inhibits tumor growth in vivo using an orthotopic xenograft breast mouse model. PMID: 28347999
  5. The novel insights into the regulation of human Lipin-1 stability will be useful in planning further studies to elucidate its metabolic processes. PMID: 28483528
  6. It seems that a new signaling axis, SIRT1-SFRS10-LPIN1 axis, acting in the pathogenesis of alcoholic fatty liver disease exists. PMID: 28467182
  7. Consistent with these observations, LPIN1 levels were positively correlated with IRS1 expression in human breast cancer. Thus, our results indicate a mechanism by which IRS1 expression is increased in breast cancer, and LPIN1 may be a promising drug target for anticancer therapy PMID: 27729374
  8. LPIN1 is upregulated in non-alcoholic fatty liver disease. Up-regulation of miR-122 can trigger the compensatory response of LPIN1 and CTDNEP1 in hepatosteatosis. PMID: 28287811
  9. lipin-1-mediated downregulation of p21 is critical for the progress of keratinocyte differentiation PMID: 26658689
  10. lipin-1 has a critical role in the regulation of macrophage inflammatory responses to modified-LDL PMID: 26288136
  11. Results of our study suggest that rs2716610: C>T polymorphism of LPIN1 gene could have a protective effect against development of metabolic syndrome, while rs11693809: C>T might affect a glucose control in patients with MS. PMID: 26276647
  12. Lipin-1 controls main cellular processes involved in cancer progression and that its targeting, alone or in combination with other treatments, could open new avenues in anticancer therapy. PMID: 25834103
  13. Lipin1beta might play a role in the pathogenesis of insulin resistance in gestational diabetes mellitus PMID: 25974997
  14. LPIN1 plays a functional role in lipid synthesis and storage, a role which is highly conserved from human to yeast. PMID: 25475986
  15. the lipin-1 N-terminal domain is important for its catalytic activity, nuclear localization, and binding to PP-1cgamma PMID: 24558042
  16. Lipin-1 deficiency is associated with lipid metabolism alterations resulting in massive rhabdomyolysis. PMID: 23928362
  17. the SNP in the LPIN1 gene (rs10192566) had no effect on the trough rosiglitazone steady-state concentration. PMID: 23426382
  18. Phosphorylation of lipin 1 and charge on the phosphatidic acid head group control its phosphatidic acid phosphatase activity and membrane association PMID: 23426360
  19. Data suggests common single nucleotide polymorphisms within the LPIN1 region were associated with Type 2 diabetes and metabolic traits in the Chinese population. PMID: 22853689
  20. LPIN1-related myolysis constitutes a major cause of early-onset rhabdomyolysis and occasionally in adults. Heterozygous LPIN1 mutations may cause mild muscular symptoms. No major defects of LPIN2 or LPIN3 genes were associated with muscle manifestations. PMID: 22481384
  21. We conclude that direct control of lipin 1 transcription by HIF-1 is an important regulatory feature of lipid metabolism and its adaptation to hypoxia. PMID: 22467849
  22. Lipin1 rs13412852 single nucleotide polymorphism is associated with the severity of liver damage and fibrosis progression in pediatric patients with histological NAFLD. PMID: 22157924
  23. LPIN1 mutations should be considered in any child presenting with severe rhabdomyolysis. PMID: 22480698
  24. Estrogen-related receptor gamma (ERRgamma) is a novel transcriptional regulator of phosphatidic acid phosphatase, LIPIN1, and inhibits hepatic insulin signaling PMID: 21911493
  25. reduced expression of SFRS10, as observed in tissues from obese humans, alters LPIN1 splicing, induces lipogenesis, and therefore contributes to metabolic phenotypes associated with obesity. PMID: 21803291
  26. Lpin1 expression in response to nutritional stress is controlled through the reactive oxygen species-ATM-p53 pathway. PMID: 22055193
  27. The aim of present study was to investigate for the first time the association of lipin 1 gene polymorphisms with metabolic and hormonal profile in polycystic ovary syndrome patients and controls. PMID: 21448847
  28. PPARG and LPIN1 gene polymorphisms role of these genes in lipid metabolism and pathogenesis of type 2 diabetes and metabolic syndrome PMID: 21263402
  29. lipin-1gamma is the main lipin-1 isoform expressed in normal human brain, suggesting a specialized role in regulating brain lipid metabolism. PMID: 21616074
  30. data suggest that lipin-1 associates with lipid droplets and regulates the activation of cytosolic group IVA phospholipase A(2)alpha in monocyte-derived macrophages PMID: 21478406
  31. dullard shows specificity for the peptide corresponding to the insulin-dependent phosphorylation site (Ser106) of lipin PMID: 21413788
  32. Data revealed that lipin 1 formed stable homo-oligomers with itself and hetero-oligomers with lipin 2/3. PMID: 20735359
  33. Results showed a marked association between LPIN1 and PPARalpha gene expression both in subcutaneous and visceral adipose tissues and might suggest a possible role of LPIN1 in stress conditions that occur in chronic obesity and underlie insulin resistance PMID: 20101248
  34. Data suggest that the high frequency of the intragenic LPIN1 deletion should provide a valuable criterion for fast diagnosis, prior to muscle biopsy. PMID: 20583302
  35. the low-grade proinflammatory environment and the insulin resistance associated with obesity may contribute to downregulate LPIN1 in adipose tissue PMID: 20530740
  36. LPIN1 rs33997857, rs6744682, and rs6708316 did not associate with type 2 diabetes, obesity, or related quantitative metabolic phenotypes in the Danish population examined. PMID: 20356931
  37. the LPIN1 gene seemed not to be a major susceptibility gene for type 2 diabetes or related metabolic phenotypes in the Chinese population. PMID: 19543209
  38. sumoylation facilitates the nuclear localization and transcriptional coactivator behavior of lipin-1alpha, which may act as a sumoylation-regulated transcriptional coactivator in brain PMID: 19753306
  39. LPIN1 mutations are not commonly seen in patients with lipodystrophy who had no mutations in known disease genes. PMID: 12111372
  40. effects of genetic variation in lipin levels on glucose homeostasis across species by analyzing lipin transcript levels in human and mouse adipose tissues PMID: 16357106
  41. human lipin 1 is a Mg2+-dependent PA phosphatase, the penultimate enzyme in the pathway to synthesize triacylglyerol PMID: 16467296
  42. These results suggest that increased adipogenesis and/or lipogenesis in subcutaneous fat, mediated by the LPIN1 gene, may prevent lipotoxicity in muscle, leading to improved insulin sensitivity PMID: 17003347
  43. Body fat accumulation is a major regulator of human adipose LPIN1 expression and suggests a role of LPIN1 in human preadipocyte as well as mature adipocyte function. PMID: 17035674
  44. insulin and epinephrine control lipin primarily by changing localization rather than intrinsic PAP activity PMID: 17105729
  45. Lipin 1beta and overexpression of peroxisome proliferator-activated receptor-gamma coactivator (PGC)-1alpha are downregulated by obesity and obesity-related metabolic perturbations in human subjects. PMID: 17563064
  46. Variants of the LPIN1 gene have significant effects in human metabolic traits and implicate lipin in the pathophysiology of the metabolic syndrome. PMID: 17940119
  47. LPIN1 messenger RNA levels positively correlated with insulin sensitivity in all subjects with obesity, NIDDM, and HIV-associated lipodystrophy PMID: 17950103
  48. Sequence variation in the LPIN1 gene contributes to variation in resting metabolic rate and obesity-related phenotypes potentially in an age-dependent manner. PMID: 18070763
  49. Lpin1 glucocorticoid response element binds to the glucocorticoid receptor PMID: 18362392
  50. Study provides further evidence that lipin 1 may play a role in blood pressure regulation, especially in men. PMID: 18437145

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Involvement in disease Myoglobinuria, acute recurrent, autosomal recessive (ARARM)
Subcellular Location Nucleus membrane, Cytoplasm, cytosol, Endoplasmic reticulum membrane
Protein Families Lipin family
Tissue Specificity Specifically expressed in skeletal muscle. Also abundant in adipose tissue. Lower levels in some portions of the digestive tract.
Database Links

HGNC: 13345

OMIM: 268200

KEGG: hsa:23175

STRING: 9606.ENSP00000256720

UniGene: Hs.467740

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