Recombinant Human Phosphatidate phosphatase LPIN2 (LPIN2), partial

Code CSB-YP838780HU
MSDS
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Source Yeast
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Code CSB-EP838780HU
MSDS
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Source E.coli
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Code CSB-EP838780HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP838780HU
MSDS
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Source Baculovirus
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Code CSB-MP838780HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
LPIN2
Uniprot No.
Alternative Names
KIAA0249; Lipin-2; Lipin2; LPIN 2; LPIN2; LPIN2_HUMAN; OTTHUMP00000162242; Phosphatidate phosphatase LPIN2
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the reticulum endoplasmic membrane. Plays important roles in controlling the metabolism of fatty acids at different levels. Acts also as a nuclear transcriptional coactivator for PPARGC1A to modulate lipid metabolism.
Gene References into Functions
  1. Structural variants unique to the malignant cell line inactivated: LPIN2, a phosphatidic acid phosphatase and a co-factor of PGC1a that is important for lipid metabolism and for suppressing autoinflammation. PMID: 23792589
  2. We describe two brothers with Majeed syndrome, homozygous novel 2-base pair deletion in LPIN2 (c.1312_1313delCT; p.Leu438fs+16X) PMID: 23087183
  3. LPIN1-related myolysis constitutes a major cause of early-onset rhabdomyolysis and occasionally in adults. Heterozygous LPIN1 mutations may cause mild muscular symptoms. No major defects of LPIN2 or LPIN3 genes were associated with muscle manifestations. PMID: 22481384
  4. role of lipin-2 in the proinflammatory action of saturated fatty acids in murine and human macrophages PMID: 22334674
  5. Data revealed that lipin 1 formed stable homo-oligomers with itself and hetero-oligomers with lipin 2/3. PMID: 20735359
  6. LPIN2 gene was excluded as a candidate for myopia 2 (MYP2), but the SNPs detected in this study will aid in future mapping and association studies involving this gene. PMID: 15862761
  7. We conclude that homozygous mutations in LPIN2 result in Majeed syndrome. Understanding the aberrant immune response in this condition will shed light on the aetiology of other inflammatory disorders of multifactorial aetiology PMID: 15994876
  8. A single nucleotide polymorphism of the LPIN2 gene is associated with type 2 diabetes and fat distribution. PMID: 17804763
  9. distinct and non-redundant functions of lipin 1 and 2 regulate lipid production during the cell cycle and adipocyte differentiation PMID: 18694939
  10. lipin 2 plays an important role as a hepatic PAP-1 enzyme. PMID: 19136718
  11. A conserved serine residue is required for the phosphatidate phosphatase activity but not the transcriptional coactivator functions of lipin-1 and lipin-2. PMID: 19717560

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Involvement in disease
Majeed syndrome (MJDS)
Subcellular Location
Nucleus. Cytoplasm, cytosol. Endoplasmic reticulum membrane.
Protein Families
Lipin family
Tissue Specificity
Expressed in liver, lung, kidney, placenta, spleen, thymus, lymph node, prostate, testes, small intestine, and colon.
Database Links

HGNC: 14450

OMIM: 605519

KEGG: hsa:9663

STRING: 9606.ENSP00000261596

UniGene: Hs.132342

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