Recombinant Human Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta (PIK3C2B), partial

Code CSB-YP017995HU
MSDS
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Source Yeast
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Code CSB-EP017995HU
MSDS
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Source E.coli
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Code CSB-EP017995HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP017995HU
MSDS
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Source Baculovirus
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Code CSB-MP017995HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PIK3C2B
Uniprot No.
Alternative Names
PIK3C2B; Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta; PI3K-C2-beta; PtdIns-3-kinase C2 subunit beta; EC 2.7.1.154; C2-PI3K; Phosphoinositide 3-kinase-C2-beta
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Phosphorylates PtdIns and PtdIns4P with a preference for PtdIns. Does not phosphorylate PtdIns(4,5)P2. May be involved in EGF and PDGF signaling cascades.
Gene References into Functions
  1. this study suggests that PIK3C2B might only have a minor role in SQCC oncogenesis PMID: 29088297
  2. PI3KC2B-silencing inhibits early stages of neuroblastoa tumorigenic growth. PMID: 25622909
  3. Data suggest a novel role for nucleotide-free Ras in cell signaling in which PI3KC2beta stabilizes nucleotide-free Ras and that interaction of Ras and PI3KC2beta mutually inhibit one another. PMID: 23028960
  4. The results of this study showed that strong association between PIK3C2B gene alternation and high-grade oligodendroglial tumors. PMID: 22825724
  5. These findings provide a unique mechanism for regulating class II PI3Ks, and identify TRIM27 as a previously undescribed negative regulator of CD4 T cells. PMID: 22128329
  6. Overexpression of PIK3C2B is associated with esophageal squamous cell carcinoma. PMID: 21986133
  7. different human small cell lung carcinoma cell lines overexpress distinct subsets of PI3Ks, resulting in differences in signalling cascades activated by stem cell factor; insights into specificity and functional significance of PI3K signalling in cancer PMID: 12356726
  8. activity of nuclear phosphoinositide 3-kinase C2beta investigated in HL-60 cells induced to differentiate along granulocytic or monocytic lineages PMID: 12372612
  9. plays a regulatory role in cell motility PMID: 16113997
  10. identified the 185-kDa protein altered by nitrotyrosylation in platelets from patients with systemic sclerosis as phosphoinositide kinase C2beta (PI 3-K); the activity of PI 3-K increases in nitrotyrosylated platelet lystaes from patients with SSc PMID: 16271833
  11. Class II phosphoinositide 3-kinase C2beta isnot essential for epidermal differentiation PMID: 16314532
  12. These findings suggest that PI3KC2beta regulates the migration and survival of human tumor cells by distinct molecular mechanisms. PMID: 16775008
  13. Epidermal growth factor stimulates the appearance of PI3K-C2beta in nuclei. PMID: 19496756
  14. Data show that the class II phosphatidylinositol 3 kinase C2beta (PI3K-C2beta) is activated by the T-cell receptor (TCR) and functions upstream of NDPK-B to activate KCa3.1 channel activity. PMID: 19587117

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Subcellular Location
Microsome. Cell membrane. Cytoplasm, cytosol. Nucleus. Endoplasmic reticulum. Note=Found mostly in the microsome, but also in the plasma membrane and cytosol. Nuclear in testis.
Protein Families
PI3/PI4-kinase family
Tissue Specificity
Expressed in columnar and transitional epithelia, mononuclear cells, and ganglion cells (at protein level). Widely expressed, with highest levels in thymus and placenta and lowest in peripheral blood, skeletal muscle and kidney.
Database Links

HGNC: 8972

OMIM: 602838

KEGG: hsa:5287

STRING: 9606.ENSP00000356155

UniGene: Hs.497487

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