Recombinant Human Phospholipase A2, membrane associated(PLA2G2A)

Code CSB-EP018091HU
Size US$1726
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names PLA2G2A
Uniprot No. P14555
Research Area Metabolism
Alternative Names EC 3.1.1.4; GIIC sPLA2; Group IIA phospholipase A2; membrane associated; Modifier of MIN 1 mouse homolog; MOM1; Non-pancreatic secretory phospholipase A2; NPS PLA2; NPS-PLA2; PA2GA_HUMAN; Phosphatidylcholine 2-acylhydrolase 2A; Phosphatidylcholine 2acylhydrolase; Phospholipase A2; Phospholipase A2 group IIA (platelets, synovial fluid); Phospholipase A2 membrane associated; Phospholipase A2 polypeptide B; Phospholipase A2 synovial; PLA2; PLA2B; PLA2G2A; PLA2L; PLA2S; PLAS1; RASF A; sPLA2
Species Homo sapiens (Human)
Source E.coli
Expression Region 21-144aa
Target Protein Sequence NLVNFHRMIKLTTGKEAALSYGFYGCHCGVGGRGSPKDATDRCCVTHDCCYKRLEKRGCGTKFLSYKFSNSGSRITCAKQDSCRSQLCECDKAAATCFARNKTTYNKKYQYYSNKHCRGSTPRC
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 17.9kDa
Protein Length Full Length of Mature Protein
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides
Gene References into Functions
  1. sPLA2 appears to play an important role in the pathogenesis of dengue. We found that sPLA2 activity was significantly higher in patients with dengue haemorrhagic fever when compared to those with dengue fever, during the first 120 h of clinical illness. PMID: 28250920
  2. The findings of this study showed that maslinic acid inhibit inflammatory effects induced by sPLA2-IIA, including foam cells formation and PGE2 production. PMID: 29872016
  3. High PLA2 activity is associated with West Nile virus infection. PMID: 29709018
  4. A lipidomics-based LC/MS assay was used to define the specificity of cPLA2, iPLA2, and sPLA2 toward a variety of phospholipids. A unique hydrophobic binding site for the cleaved fatty acid dominates each enzyme's specificity rather than its catalytic residues and polar headgroup binding site. PMID: 29342349
  5. demonstration of an independent association between early-stage atherosclerosis and increased levels of sPLA2-IIa, implying that increased sPLA2-IIa may have a role in early-stage atherosclerosis in MetS patients PMID: 27941821
  6. these results suggest that PLA2G2A polymorphisms are involved in the risk of developing metabolic syndrome and type 2 diabetes mellitus and are associated with subclinical atherosclerosis in this group of patients PMID: 27608594
  7. PLA2G2A has a previously undiscovered impact on insulin sensitivity and metabolism PMID: 28663239
  8. lipidomic analyses revealed that sequestration of PUFAs in LDs by sPLA2-induced TAG remodelling and retention of PUFAs in LDs by inhibition of ATGL-mediated TAG lipolysis protect from PUFA lipotoxicity. PMID: 29229414
  9. This report provides the first demonstration that Phosphatidylcholine-Isoprostanes are readily hydrolyzed by group IIA, V and X Secretory Phospholipases A2. PMID: 28528433
  10. Given an aberrant high level of sPLA2IIa in the tumor microenvironment that should be much higher than that in the blood, our findings support the notion that sPLA2IIa functions as a ligand for EGFR family receptors and supports CSC properties via HER/ERBB-elicited signaling, which may contribute to resistance to therapy and cancer progression PMID: 28440478
  11. the activity of human cPLA2alpha towards a multitude of glycerophospholipids species present in micelles or bilayers, was investigated. PMID: 27377346
  12. Mutational analysis of functional sites showed that both peroxidase and PLA2 active sites were necessary for mutant Prdx6 function, and that Prdx6 phosphorylation (at T177 residue) was essential for optimum PLA2 activity.Mutant Prdx6 at its Sumo1 sites escapes and abates this adverse process by maintaining its integrity and gaining function PMID: 28055018
  13. sPLA2-IIA activates Integrin alphaVbeta3 and Integrin alpha4beta1 in an allosteric manner. (Review) PMID: 27864802
  14. Leptin, at concentrations with minimal or no activating effects on astrocytoma cells, enhanced growth and migration promoted by low doses of sPLA2-IIA. sPLA2-IIA alone induced a transient phosphorylation pattern in the Src/ERK/Akt/mTOR/p70S6K/rS6 pathway through EGFR transactivation PMID: 28249041
  15. Molecular dynamics simulations reveal structural insights into inhibitor binding modes and functionality in human PLA2G2A. PMID: 28056488
  16. Hepatitis B virus can upregulate the expression of PLA2G2A, and serum levels of PLA2G2A are associated with the progression of hepatitis to liver cirrhosis and hepatocellular carcinoma. PMID: 28077172
  17. Dimethyl ester of bilirubin exhibits anti-inflammatory activity through inhibition of secretory phospholipase A2, lipoxygenase and cyclooxygenase. PMID: 27060751
  18. High expression of PLA2G2A was associated with poor therapeutic response and worse survival in patients with rectal cancer receiving neoadjuvant concurrent cheomoradiotherapy (CCRT). PMID: 25393083
  19. Data show that phospholipase A2 group IIA, V and X have different target/function related activity. PMID: 26711221
  20. Polyozellin might play an important role in the modulation of sPLA2-IIA expression and activity in response to the inflammatory diseases. PMID: 26659873
  21. Overexpression of sPLA2-IIA does not alter MCP-1 level at baseline, but could enhance the atherogenic effect of oxidized LDL in HASMCs, at least partly due to activation of Akt. PMID: 26488172
  22. These results suggest that the genetic ablation of iPLA2b increased iron uptake in the brain through the activation of IRP2 and upregulation of DMT1, which may be associated with mitochondrial dysfunction. PMID: 26506412
  23. IL-22 induced at an early stage of L. monocytogenes infection enhances innate immunity against L. monocytogenes in the liver by stimulating hepatocytes to produce PLA2G2A PMID: 26644377
  24. PLA2G2A was detected in all samples of endometriomas, but not in normal endometrium, and immunohistochemistry showed PLA2G2A-specific staining in epithelial cells of endometrioma paraffin sections. PMID: 25366587
  25. Results indicate that secreted phospholipase A2 IIA (sPLA2-IIA) as an enzyme working in concert with platelet microparticles (MPs) 12-lipoxygenase (12-LO) to promote internalization. PMID: 26106157
  26. Secretory PLA2 IIa expression can predict the clinical outcome of lung adenocarcinoma patients. PMID: 25317799
  27. Co-expression of PLA2G2A and COX-2 significantly correlated with the depth of invasion (p=0.004)and with TNM stage (p=0.04) of esophageal squamous cell carcinoma PMID: 25422234
  28. sPLA2 are secreted from ciliated cells and appear to induce mucin and cysLT secretion from goblet cells, strongly suggesting that airway goblet cells are proinflammatory effector cells. PMID: 25429648
  29. Studies indicate that the expression of secreted phospholipases A2 (sPLA2s), most notably the group IIA, III and X enzymes, is dysregulated in various malignant tissues. PMID: 25286228
  30. PLA2G2A levels increase in expectorations of cystic fibrosis patients in an age-dependent manner. PMID: 25290234
  31. AdPLA2 plays an important role in promoting tumorigenesis and disease progression by modulating the production of prostaglandins and may serve as a potential therapeutic target in TSC and LAM. PMID: 25347447
  32. Summarizing, Der p1 and Fel d1 involve phospholipase A2 enzymes in their action. PMID: 25247183
  33. The minor allele of rs11573156, is a dominant haplotype and is associated with the higher sPLA2-IIa levels. PMID: 25794429
  34. Activity of iPLbeta correlates inversely with the hydrophobicity of the phospholipid substrate. PMID: 25713085
  35. Secretory phospholipase A2 activity is raised in acute respiratory distress syndrome infants. PMID: 23883784
  36. Proinflammatory secreted phospholipase A2 type IIA (sPLA-IIA) induces integrin activation through direct binding to a newly identified binding site (site 2) in integrin alphaVbeta3, integrin alphavbeta1, and integrin alpha4beta1. PMID: 25398877
  37. women with metabolic syndrome demonstrated a low but positive correlation between sPLA2 and hsCRP levels PMID: 24521628
  38. Findings support the notion that sPLA2-IIa functions as a ligand for the EGFR family of receptors leading to an elevated HER/HER2-elicited signaling lung cancer cells. PMID: 24913497
  39. PLA2G2A gene polymorphisms may modify the risk of esophageal squamous cell carcinoma development PMID: 24641411
  40. hydrolysis of the mitochondrial membrane by sPLA2-IIA yields inflammatory mediators (ie, lysophospholipids, fatty acids, and mtDNA) that promote leukocyte activation PMID: 25082876
  41. This review presents evidence for pathophysiological roles of sPLA2 in the central nervous system and neurological diseases. PMID: 24113843
  42. Determined circulating levels of sPLA2-IIA, C-reactive protein (CRP), fibrinogen, factor VIII (FVIII), von Willebrand factor (vWF), and antithrombin as biomarkers of inflammation and coagulation in patients with various types of malignancies. PMID: 23722320
  43. Increased serum levels of serum amyloid a, PLA2G2, and C reactive protein were observed in patients with benign prostate hyperplasia, localized and metastasized prostate cancer in comparison to healthy individuals. PMID: 24061501
  44. Data indicate that the expression of genes encoding hGIIA, hGIII and hGX sPLA2s (PLA2G2A, PLA2G3 and PLA2G10, respectively) in breast tumour biopsies differs from that in normal tissues. PMID: 24508801
  45. two of these isoforms, sPLA2 IIA and sPLA2 IIF, localize to the upper stratum granulosum and increase in response to experimental barrier perturbation. PMID: 24269828
  46. Studied the role of human sPLA2-IIA, a bactericidal enzyme induced during acute inflammation, in innate immunity against GBS, and show clinical GBS isolates are highly sensitive to killing by sPLA2-IIA but not by human antimicrobial peptides. PMID: 23901095
  47. In a humanized triple transgenic mouse model with hypercholesterolemia, sPLA2-IIA inhibition increases CETP activity via increasing the very low-density lipoprotein-apoE content, resulting in a proatherogenic lipoprotein profile. PMID: 24115030
  48. Group IIA phospholipase A2 expression is an independent predictor of favorable outcome for patients with gastric cancer. PMID: 23664539
  49. Plasma PLA2 activities had approximately 70% specificity and sensitivity to detect cancer PMID: 23451150
  50. PLA2G2A may be a useful therapeutic target for a subset of gastric cancers PMID: 23852891
  51. Halogenated phospholipids are not the sPLA2 substrates. Halogenated phospholipids inhibit the sPLA2 activity. PMID: 23438648
  52. an association between vimentin, hGIIA, and arachidonic acid metabolism in synovial inflammation. PMID: 23482564
  53. PLA2G2A expression inversely correlated with pathological classification. PMID: 23609358
  54. Investigation into the role of phosphatidylserine in modifying the susceptibility of lymphocytes to secretory phospholipase A(2) using cells deficient in the expression of scramblase. PMID: 22266334
  55. Hydrophobic residues Leu2, Val3, Ala18, Leu19, Phe23, Gly30, and Phe63 that form the edge of the entrance of the hydrophobic binding pocket in PLA2 tend to penetrate into the hydrophobic area of lipid bilayers. PMID: 23343574
  56. membrane oxidation has a role in controlling the activity of human group IIa secretory phospholipase A(2) toward apoptotic lymphoma cells PMID: 22989727
  57. Baseline sPLA(2)IIa expression predicts response to sPLA(2)IIa inhibition in some K-ras mutant lung cancer cells. PMID: 23026567
  58. Knockdown of secretory PLA2 IIa suppresses lung cancer growth in part by attenuating NF-kappaB activity. PMID: 23079010
  59. mRNA expressionsof the gene encoding sPLA2G2A are significantly higher in recurrent depressive disorder than in controls. PMID: 22331023
  60. The data obtained indicate that sPLA(2)IIA may contribute to the pathogenesis of neurodegenerative diseases involving neuroinflammation PMID: 22406427
  61. sPLA2-mediated hydrolysis of surfactant phospholipid, especially phosphatidylglycerol (PG) by PLA2G2A, contributes to surfactant injury during early acute respiratory distress syndrome (ARDS). PMID: 22173044
  62. High levels of plasma secretory PLA2-IIa may serve as a tumor prognostic biomarker capable of distinguishing aggressive from indolent prostate cancers. PMID: 22127954
  63. Lp-PLA(2) antigen but not activity is significantly associated with faster rates of kidney function decline PMID: 22056971
  64. stimulated platelets are major contributors to plasma sPLA2 activity in vivo and account to a large extent for the adverse modification of circulating lipoproteins PMID: 22042222
  65. This study found that patients with TLE-MTS and psychosis had a significantly increased calcium-independent PLA(2) activity as compared to patients without psychosis PMID: 21813137
  66. This study shows that T cells and antibodies may show cross-reactivity between different species without being naturally exposed to sPLA2s. PMID: 21848517
  67. Human sPLA2-IIA contributes to the host defense against serious experimental S. pyogenes infection, providing a novel example of a bacterial resistance mechanism involved in protection against S. pyogenes infections in humans. PMID: 22075700
  68. PLA2G2A may predict survival and might be a potential biomarker for early detection and individualized therapy. PMID: 21884198
  69. Inhibitory effect of thrombin on the expression of secretory group IIA phospholipase A2 PMID: 21544859
  70. genetic polymorphisms, determination of serum sPLA(2)-IIa, and prognosis in coronary heart disease patients PMID: 21799821
  71. Multiple phospholipase A2 enzymes participate in the inflammatory process in osteoarthritic cartilage. PMID: 21417548
  72. Serum sPLA(2) activity has a role in abdominal aortic aneurysm (AAA) in men with small AAAs but is not associated with AAA progression PMID: 21382622
  73. sPLA(2) -IIA and sPLA(2) -X are the major sPLA(2) s in human airways, and suggest a link between the levels of sPLA(2) -X in the airways and several features of asthma. PMID: 21255140
  74. These data revealed that Vav3 overexpression as an additional underlying mechanism contributes to elevated sPLA2-IIa expression in prostate cancer PMID: 21455584
  75. Data suggests that secretory sPLA2 IIA may be expressed in islets during insulitis, but not expressed in islets without insulitis. PMID: 21099324
  76. sPLA2-IIA, CRP, and C3d are significantly more activated in atherosclerotic aortic valves compared to degeneratively changed aortic valves. PMID: 21404895
  77. A bifunctional role for group IIA secreted phospholipase A2 in human rheumatoid fibroblast-like synoviocyte arachidonic acid metabolism. PMID: 21068383
  78. The content of sPLA2-IIa in tears of seasonal allergic conjunctivitis and perennial allergic conjunctivitis patients was elevated. PMID: 21042565
  79. results suggest that the tumor cell iPLAbeta-lysophosphatidic acid axis may represent a novel target for prostate cancer PMID: 20812222
  80. This study showed that sPLA(2)-IIA is able to bring into play EGFR to trigger its signaling PMID: 20639215
  81. Data show that PLAforms could serve as markers of the immunity status in ARDS. PMID: 20600872
  82. Secretory PLA2-IIa overexpression is associated with prostate development and progression PMID: 20837598
  83. Secretory phospholipase A2 was found to be associated with degree of severity of coronary artery disease. PMID: 19449149
  84. Data indicate that PLA2G2A does not contribute to the reduced reverse cholesterol transport observed during acute phase responses; PLA2G2A does contribute to reduced cholesterol efflux toward plasma. PMID: 20061576
  85. Secretory PLA2 plays a novel anti-inflammatory role in rheumatoid arthritis PMID: 20432503
  86. Group IIA secreted phospholipase A2 induces production of VEGF-A and VEGF-C in lung macrophages by a receptor-mediated, catalytically independent mechanism and may play an important role in inflammatory and neoplastic angiogenesis and lymphangiogenesis. PMID: 20357262
  87. The antibacterial activity of phospholipase A(2) type IIA is regulated by the cooperative lipid chain melting behavior in Staphylococcus aureus PMID: 19962957
  88. End-stage renal disease patients show increased formation of COX2-derived prostaglandins (p < 0.05) correlated with plasma sPLA2 (r = 0.71, p < 0.05). PMID: 19798476
  89. Secretory type II phospholipase A2 is produced and secreted by epicardial adipose tissue and overexpressed in patients with coronary artery disease. PMID: 20008021
  90. sPLA(2)-IIA and TNFalpha transduction pathways interact to modulate inflammatory responses and provide additional insights about the capacity of sPLA(2)-IIA to promote apoptosis resistance in astrocytoma cells. PMID: 19850087
  91. this review highlights the potential role of the IIA PLA2 in resolving inflammatory situations involving both catalytic (antibacterial) and non-catalytic (debris removal) functions.[Review] PMID: 19818633
  92. analysis of the molecular mechanism of human group IIA phospholipase A2 inactivation by bolinaquinone PMID: 19621421
  93. Native and oxidized lipoproteins regulate catalytic activity of sPLA(2)(IIA). PMID: 19647092
  94. data are consistent with the contention that group IIA sPLA2 expression is elevated in neoplastic prostatic tissue and support the hypothesis that dysregulation of sPLA2 may play a role in prostatic carcinogenesis PMID: 11839587
  95. Ceramides increase the activity of the secretory phospholipase A2 and alter its fatty acid specificity PMID: 11903045
  96. antibacterial properties of PLA2-IIA in human acute phase serum PMID: 12085323
  97. determination of binding site preference for membranes with anionic phospholipids PMID: 12244093
  98. the potent antistaphylococcal activity of Group IIA PLA(2) depends on cationic properties of the enzyme that promote binding to the cell wall PMID: 12359734
  99. The activity of PLA2G2A may suppress progression or metastasis of human gastric cancer. PMID: 12456890
  100. The crystal structure of H48Q PLA2 reveals that the active site structure is maintained intact in the mutant and suggests a mechanism in which it is not essential to involve a base (His-48) to deprotonate a water molecule in order to facilitate catalysis. PMID: 12501175

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Subcellular Location Cell membrane, Peripheral membrane protein, Secreted
Protein Families Phospholipase A2 family
Database Links

HGNC: 9031

OMIM: 172411

KEGG: hsa:5320

STRING: 9606.ENSP00000364252

UniGene: Hs.466804

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