Recombinant Human Phosphoribosylformylglycinamidine synthase (PFAS), partial

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Code CSB-EP017811HU
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
PFAS
Uniprot No.
Alternative Names
PFAS; KIAA0361; Phosphoribosylformylglycinamidine synthase; FGAM synthase; FGAMS; EC 6.3.5.3; Formylglycinamide ribonucleotide amidotransferase; FGAR amidotransferase; FGAR-AT; Formylglycinamide ribotide amidotransferase
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
1064-1302aa
Target Protein Sequence
RVAILREEGSNGDREMADAFHLAGFEVWDVTMQDLCSGAIGLDTFRGVAFVGGFSYADVLGSAKGWAAAVTFHPRAGAELRRFRKRPDTFSLGVCNGCQLLALLGWVGGDPNEDAAEMGPDSQPARPGLLLRHNLSGRYESRWASVRVGPGPALMLRGMEGAVLPVWSAHGEGYVAFSSPELQAQIEARGLAPLHWADDDGNPTEQYPLNPNGSPGGVAGICSCDGRHLAVMPHPERAV
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
45.4 kDa
Protein Length
Partial
Tag Info
N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

This Human PFAS recombinant protein was produced in E.coli, where the gene sequence encoding Human PFAS (1064-1302aa) was expressed with the N-terminal 10xHis-SUMO tag and C-terminal Myc tag. The purity of this PFAS protein was greater than 85% by SDS-PAGE.
PFAS is part of the purine biosynthesis pathway, and its specific role in this pathway is to convert multiple substrates into 5-phosphoribosylformylglycinamidine (FGAR). This reaction is a critical step in purine synthesis. Purines are essential components of DNA and RNA and serve as precursors to important energy molecules like ATP and GTP within cells. PFAS's involvement in purine synthesis ensures that cells can manufacture an adequate supply of purines to support their survival and growth. The activity of PFAS is regulated by various factors, including substrate concentrations, feedback inhibition, and the actions of regulatory proteins. This precise regulation helps maintain the balance of the purine biosynthesis pathway.

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Target Background

Function
Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.
Subcellular Location
Cytoplasm.
Protein Families
FGAMS family
Database Links

HGNC: 8863

OMIM: 602133

KEGG: hsa:5198

STRING: 9606.ENSP00000313490

UniGene: Hs.573976

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