Recombinant Human Phosphoribosylformylglycinamidine synthase (PFAS), partial

This Human PFAS recombinant protein was produced in E.coli, where the gene sequence encoding Human PFAS (1064-1302aa) was expressed with the N-terminal 10xHis-SUMO tag and C-terminal Myc tag. The purity of this PFAS protein was greater than 85% by SDS-PAGE.
PFAS is part of the purine biosynthesis pathway, and its specific role in this pathway is to convert multiple substrates into 5-phosphoribosylformylglycinamidine (FGAR). This reaction is a critical step in purine synthesis. Purines are essential components of DNA and RNA and serve as precursors to important energy molecules like ATP and GTP within cells. PFAS's involvement in purine synthesis ensures that cells can manufacture an adequate supply of purines to support their survival and growth. The activity of PFAS is regulated by various factors, including substrate concentrations, feedback inhibition, and the actions of regulatory proteins. This precise regulation helps maintain the balance of the purine biosynthesis pathway.