Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Activity

Not Test

Target Names

HDAC10

Uniprot No.

Q969S8

Research Area

Epigenetics and Nuclear Signaling

Alternative Names

Histone deacetylase 10;HD10

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-669aa

Target Protein Sequence

MGTALVYHEDMTATRLLWDDPECEIERPERLTAALDRLRQRGLEQRCLRLSAREASEEELGLVHSPEYVSLVRETQVLGKEELQALSGQFDAIYFHPSTFHCARLAAGAGLQLVDAVLTGAVQNGLALVRPPGHHGQRAAANGFCVFNNVAIAAAHAKQKHGLHRILVVDWDVHHGQGIQYLFEDDPSVLYFSWHRYEHGRFWPFLRESDADAVGRGQGLGFTVNLPWNQVGMGNADYVAAFLHLLLPLAFEFDPELVLVSAGFDSAIGDPEGQMQATPECFAHLTQLLQVLAGGRVCAVLEGGYHLESLAESVCMTVQTLLGDPAPPLSGPMAPCQSALESIQSARAAQAPHWKSLQQQDVTAVPMSPSSHSPEGRPPPLLPGGPVCKAAASAPSSLLDQPCLCPAPSVRTAVALTTPDITLVLPPDVIQQEASALREETEAWARPHESLAREEALTALGKLLYLLDGMLDGQVNSGIAATPASAAAATLDVAVRRGLSHGAQRLLCVALGQLDRPPDLAHDGRSLWLNIRGKEAAALSMFHVSTPLPVMTGGFLSCILGLVLPLAYGFQPDLVLVALGPGHGLQGPHAALLAAMLRGLAGGRVLALLEENSTPQLAGILARVLNGEAPPSLGPSSVASPEDVQALMYLRGQLEPQWKMLQCHPHLVA
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

75.5 kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant human polyamine deacetylase HDAC10 is co-expressed with the N-terminal 6xHis-tag in E.coli. Its expression region corresponds to the 1-669aa of the human HDAC10 protein. It is subjected to Ni column affinity chromatography purification. Its purity exceeds 85% as determined by SDS-PAGE.

HDAC10 is crucial for various biological processes, including transcriptional regulation, cell cycle development, hormone regulation, lipid metabolism, and autophagy [1][2]. HDAC10 promotes gene transcription repression by forming a protein complex with nuclear receptor co-repressor (NCOR) proteins [3]. Recent studies have highlighted the significance of HDAC10 in different diseases, such as cancer. HDAC10 has been implicated in promoting cancer progression and resistance to therapy [4]. HDAC10 is linked to angiogenesis in endothelial cells through specific signaling pathways [5]. Furthermore, HDAC10 also regulates the cell cycle G2/M phase transition through specific molecular pathways [6].

References:
[1] I. Oehme, J. Linke, B. Böck, T. Milde, M. Lodrini, B. Hartensteinet al., Histone deacetylase 10 promotes autophagy-mediated cell survival, Proceedings of the National Academy of Sciences, vol. 110, no. 28, 2013. https://doi.org/10.1073/pnas.1300113110
[2] Y. Xiang, Myeloid-specific hdac10 deletion protects against lps-induced acute lung injury via p62 acetylation at lysine 165, Respiratory Research, vol. 25, no. 1, 2024. https://doi.org/10.1186/s12931-024-02891-2
[3] X. Jin, Y. Yan, D. Wang, D. Ding, T. Ma, Z. Yeet al., Dub3 promotes bet inhibitor resistance and cancer progression by deubiquitinating brd4, Molecular Cell, vol. 71, no. 4, p. 592-605.e4, 2018. https://doi.org/10.1016/j.molcel.2018.06.036
[4] Y. Li, X. Zhang, S. Zhu, E. Dejene, W. Peng, A. Sepulvedaet al., Hdac10 regulates cancer stem-like cell properties in kras-driven lung adenocarcinoma, Cancer Research, vol. 80, no. 16, p. 3265-3278, 2020. https://doi.org/10.1158/0008-5472.can-19-3613
[5] B. Duan, D. Ye, S. Zhu, W. Jia, C. Lu, G. Wanget al., Hdac10 promotes angiogenesis in endothelial cells through the ptpn22/erk axis, Oncotarget, vol. 8, no. 37, p. 61338-61349, 2017. https://doi.org/10.18632/oncotarget.18130
[6] Y. Li, L. Peng, & E. Seto, Histone deacetylase 10 regulates the cell cycle g2/m phase transition via a novel let-7–hmga2–cyclin a2 pathway, Molecular and Cellular Biology, vol. 35, no. 20, p. 3547-3565, 2015. https://doi.org/10.1128/mcb.00400-15

Target Background

Function

Polyamine deacetylase (PDAC), which acts preferentially on N(8)-acetylspermidine, and also on acetylcadaverine and acetylputrescine. Exhibits attenuated catalytic activity toward N(1),N(8)-diacetylspermidine and very low activity, if any, toward N(1)-acetylspermidine. Histone deacetylase activity has been observed in vitro. Has also been shown to be involved in MSH2 deacetylation. The physiological relevance of protein/histone deacetylase activity is unclear and could be very weak. May play a role in the promotion of late stages of autophagy, possibly autophagosome-lysosome fusion and/or lysosomal exocytosis in neuroblastoma cells. May play a role in homologous recombination. May promote DNA mismatch repair.

Gene References into Functions

HDAC10 structure and molecular function as a polyamine deacetylase has been reported. PMID: 28516954
this study demonstrated that HDAC10 localizes and functions in the cytoplasm of lung cancer cells, thereby underscoring its potential role in the diagnosis and treatment of lung cancer PMID: 27449083
HDAC10 regulates cyclin A2 expression by deacetylating histones near the let-7 promoter. PMID: 26240284
study identifies an HDAC10-mediated regulatory mechanism controlling the DNA mismatch repair function of MSH2. PMID: 26221039
Suggest HDAC10 expression as a prognostic marker for gastric cancer. PMID: 25337229
this study is describing HDAC10 as a promoter of autophagy-mediated survival in neuroblastoma cells and identifying this HDAC isozyme as a druggable regulator of advanced-stage tumor cell survival. PMID: 24145760
HDAC10 suppresses expression of matrix metalloproteinase (MMP) 2 and 9 genes, which are known to be critical for cancer cell invasion and metastasis PMID: 23897811
results demonstrate that HDAC10 protects cancer cells from cytotoxic agents by mediating autophagy and identify this HDAC isozyme as a druggable regulator of advanced-stage tumor cell survival PMID: 23801752
Histone deacetylases 9 and 10 are required for homologous recombination. PMID: 21247901
HDAC10 is involved in transcriptional downregulation of TXNIP, leading to altered reactive oxygen species signaling in human gastric cancer cells. PMID: 20680488
Results not only show that HDAC10 regulates melanogenesis but also demonstrate that the transcriptional activities of Pax3 and KAP1 are intimately linked to their acetylation status. PMID: 20032463
another histone deacetylase PMID: 11739383
molecular cloning and characterization of a novel class II histone deacetylase PMID: 11861901
Reduced expression of histone deacetylase 10 is associated lung cancer PMID: 15305372
Results suggest that the "T" allele of HDAC10-589C>T affect on the increased transcription activity, and might accelerate HCC development through increased expression of HDAC10. PMID: 17892858

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Subcellular Location

Cytoplasm. Nucleus.

Protein Families

Histone deacetylase family, HD type 2 subfamily

Tissue Specificity

Widely expressed with high levels in liver and kidney.

Database Links

HGNC: 18128

OMIM: 608544

KEGG: hsa:83933

STRING: 9606.ENSP00000216271

UniGene: Hs.26593

Code	CSB-EP010236HU
Abbreviation	Recombinant Human HDAC10 protein
MSDS	MSDS Datasheet
Size	$256
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Polyamine deacetylase HDAC10 (HDAC10)

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