Recombinant Human Pre-mRNA cleavage complex 2 protein Pcf11 (PCF11), partial

Code CSB-YP017603HU
MSDS
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Source Yeast
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Code CSB-EP017603HU
MSDS
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Source E.coli
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Code CSB-EP017603HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP017603HU
MSDS
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Source Baculovirus
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Code CSB-MP017603HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PCF11
Uniprot No.
Alternative Names
hPCF11; KIAA0824; OTTHUMP00000234826; PCF11; PCF11 cleavage and polyadenylation factor subunit; PCF11; cleavage and polyadenylation factor II subunit; homolog ; PCF11; cleavage and polyadenylation factor subunit; homolog (S.; PCF11_HUMAN; PCF11p homolog ; Pre-mRNA cleavage complex 2 protein Pcf11; Pre-mRNA cleavage complex II protein Pcf11
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of pre-mRNA cleavage complex II.
Gene References into Functions
  1. WNK1 and the associated phosphorylation of the PCF11 CID act to promote transcript release from chromatin-associated Pol II, which in turn facilitates mRNA export to the cytoplasm PMID: 29196535
  2. Pcf11 can act as a negative elongation factor to repress RNA Pol II gene expression in eukaryotic cells. PMID: 17606639
  3. Pcf11 is required for the efficient degradation of the 3' product of poly(A) site cleavage. PMID: 18086705
  4. Human PCf11 is a pre-mRNA processing factor. PMID: 11060040
Subcellular Location
Nucleus.
Database Links

HGNC: 30097

OMIM: 608876

KEGG: hsa:51585

STRING: 9606.ENSP00000298281

UniGene: Hs.128959

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