Recombinant Human Prefoldin subunit 5 (PFDN5)

In Stock
Code CSB-EP017815HU
MSDS
Size $224
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
PFDN5
Uniprot No.
Research Area
Epigenetics and Nuclear Signaling
Alternative Names
1190001O17Rik; 1700010A06Rik; c myc binding protein; c-myc binding protein MM 1; C-myc-binding protein Mm-1; D15Ertd697e; EIG 1; Eig1; MGC5329; MGC71907; MM 1; MM1; Myc modulator 1; PFD5; PFD5_HUMAN; PFDN5; Prefoldin 5; Prefoldin subunit 5
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
2-154aa
Target Protein Sequence
AQSINITELNLPQLEMLKNQLDQEVEFLSTSIAQLKVVQTKYVEAKDCLNVLNKSNEGKELLVPLTSSMYVPGKLHDVEHVLIDVGTGYYVEKTAEDAKDFFKRKIDFLTKQMEKIQPALQEKHAMKQAVMEMMSQKIQQLTALGAAQATAKA
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
44.2kDa
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal GST-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. Represses the transcriptional activity of MYC.
Gene References into Functions
  1. Results from a study on gene expression variability markers in early-stage human embryos shows that PFDN5 is a putative expression variability marker for the 3-day, 8-cell embryo stage. PMID: 26288249
  2. interaction with p73 PMID: 11844794
  3. MM-1alpha, MM-1gamma, and MM-1gamma, but not MM-1beta, were found to repress transcription activity of c-Myc PMID: 16173081
  4. Wnt4 gene, a member of the Wnt-beta-catenin pathway, was identified as a target gene of MM-1. PMID: 18281035
Subcellular Location
[Isoform 1]: Nucleus.; [Isoform 2]: Cytoplasm.; [Isoform 3]: Nucleus.
Protein Families
Prefoldin subunit alpha family
Tissue Specificity
Highly expressed in pancreas and skeletal muscle and moderately in other tissues.
Database Links

HGNC: 8869

OMIM: 604899

KEGG: hsa:5204

STRING: 9606.ENSP00000334188

UniGene: Hs.655327

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