Recombinant Human Prestin (SLC26A5), partial,Yeast

Code CSB-YP021528HU
MSDS
Size $250
Order now
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
SLC26A5
Uniprot No.
Research Area
Neuroscience
Alternative Names
SLC26A5; PRESPrestin; Solute carrier family 26 member 5
Species
Homo sapiens (Human)
Source
Yeast
Mol. Weight
29.0kDa
Protein Length
Cytoplasmic Domain
Tag Info
N-terminal His-tagged
Tag-Free
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Motor protein that converts auditory stimuli to length changes in outer hair cells and mediates sound amplification in the mammalian hearing organ. Prestin is a bidirectional voltage-to-force converter, it can operate at microsecond rates. It uses cytoplasmic anions as extrinsic voltage sensors, probably chloride and bicarbonate. After binding to a site with millimolar affinity, these anions are translocated across the membrane in response to changes in the transmembrane voltage. They move towards the extracellular surface following hyperpolarization, and towards the cytoplasmic side in response to depolarization. As a consequence, this translocation triggers conformational changes in the protein that ultimately alter its surface area in the plane of the plasma membrane. The area decreases when the anion is near the cytoplasmic face of the membrane (short state), and increases when the ion has crossed the membrane to the outer surface (long state). So, it acts as an incomplete transporter. It swings anions across the membrane, but does not allow these anions to dissociate and escape to the extracellular space. Salicylate, an inhibitor of outer hair cell motility, acts as competitive antagonist at the prestin anion-binding site.
Gene References into Functions
  1. Our study thus provides experimental evidence that supports a causal relationship between the R130S mutation in the prestin gene and hearing loss found in patients with this missense mutation. PMID: 27041369
  2. Prestin expression imparts susceptibility to 2-hydroxypropyl-beta-cyclodextrin-induced hearing loss. PMID: 26903308
  3. I hypothesize that serum assays of OHC specific protein, prestin, will allow detection and quantification of OHC damage before audiometric testing can identify presence of hearing loss. PMID: 25920562
  4. anion-pi interaction is the mechanism for the voltage-dependent response of prestin PMID: 26283790
  5. The findings suggest that CASK and the truncated prestin splice isoform contribute to confinement of prestin to the basolateral region of the plasma membrane. PMID: 23542924
  6. The effects of fast temperature jumps induced by an infrared (IR) laser in control and prestin (SLC26a5)-transfected human embryonic kidney (HEK) cells, are reproted. PMID: 24138858
  7. Calmodulin-prestin interaction may be involved in the medial olivocochlear-mediated modulation of cochlear amplification PMID: 24453323
  8. COCH and SLC26A5 mRNA are expressed in specific structures and cells of the inner ear in archival human temporal bone PMID: 23660400
  9. prestin subunits are individually functional within a given multimer PMID: 23212912
  10. This result implies that in cell membranes prestin oligomerizes to a tetramer. PMID: 21975444
  11. Four mutations (C124A, C192A, C260A, and C415A), all in nonconserved cysteinyl residues, significantly differed in their nonlinear capacitance properties compared with wild-type prestin. PMID: 21813750
  12. Observational study of genetic testing. (HuGE Navigator) PMID: 20668687
  13. These data reveal that the STAS (sulfate transporters and anti-sigma factor antagonist) domain starts immediately after the last transmembrane segment and lies beneath the lipid bilayer. PMID: 20471983
  14. Cysteine mutagenesis reveals transmembrane residues associated with charge translocation in prestin PMID: 19926791
  15. an essential function of prestin in human auditory processing PMID: 12719379
  16. KCNQ4 phosphorylation via PKA and coupling to a complex that may include prestin can lead to the negative activation and the negative resting potential found in adult outer hair cells. PMID: 15660259
  17. Because mammals possess differentiated outer hair cells (OHC), they also benefit from a novel electromotile process, powered by the motor protein, prestin. PMID: 16873410
  18. Packing of helices and interactions between residues surrounding the sulfate transporter motif is essential for normal prestin activity. PMID: 17151276
  19. This is the first genetic and electrophysiological analysis of a human mutation in a coding exon of the pres gene by 47 patients with non-syndromic, sensorineural, mild-to-moderate hearing impairment. PMID: 17786286
  20. Cholesterol levels modulate the distribution of prestin within plasma membrane microdomains and affect prestin self-association PMID: 17933870
  21. results indicate that an evolutionarily conserved dimeric quaternary structure represents the native and functional state of SLC26 transporters PMID: 18073211
  22. Results quantify the relation between membrane cholesterol concentration and prestin-associated charge movement and enhance our understanding of how membrane composition modulates prestin function. PMID: 18567583
  23. heterozygosity for the mutation IVS2-2A>G in SLC26A5 gene may not, by itself, be sufficient to cause hearing loss. PMID: 19027966
  24. analysis of novel DNA sequence variations in SLC26A5, encoding prestin PMID: 19492055
  25. Results are consistent with significant movements in the COOH-terminal domain of prestin upon change in membrane potential, providing the first dynamic information on its molecular rearrangements. PMID: 19515900
  26. This indicates that Met-225 in prestin somehow adjusts nonlinear capacitance and the motility of prestin-expressing cells. PMID: 19737539
  27. Observational study of gene-disease association. (HuGE Navigator) PMID: 19492055
  28. Observational study of gene-disease association. (HuGE Navigator) PMID: 19027966
  29. Observational study of gene-disease association. (HuGE Navigator) PMID: 18988928
  30. Observational study of genotype prevalence. (HuGE Navigator) PMID: 16086836

Show More

Hide All

Involvement in disease
Deafness, autosomal recessive, 61 (DFNB61)
Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
SLC26A/SulP transporter (TC 2.A.53) family
Database Links

HGNC: 9359

OMIM: 604943

KEGG: hsa:375611

STRING: 9606.ENSP00000304783

UniGene: Hs.585146

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1
Place an order now

I. Product details

*
*
*
*

II. Contact details

*
*

III. Ship To

*
*
*
*
*
*
*

IV. Bill To

*
*
*
*
*
*
*
*