Product Details

Purity

>85% (SDS-PAGE)

Target Names

PRICKLE1

Uniprot No.

Q96MT3

Alternative Names

PRICKLE1; RILPPrickle-like protein 1; REST/NRSF-interacting LIM domain protein 1

Species

Homo sapiens (Human)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Involved in the planar cell polarity pathway that controls convergent extension during gastrulation and neural tube closure. Convergent extension is a complex morphogenetic process during which cells elongate, move mediolaterally, and intercalate between neighboring cells, leading to convergence toward the mediolateral axis and extension along the anteroposterior axis. Necessary for nuclear localization of REST. May serve as nuclear receptor.

Gene References into Functions

upregulation of PRICKLE1 in basal breast cancers, a subtype characterized by high metastatic potential, is associated with poor metastasis-free survival. PMID: 27184734
these findings suggest PRICKLE1 mutations contribute to ASD by disrupting the interaction with SYN1 and regulation of synaptic vesicles. PMID: 24312498
study demonstrates that PRICKLE1 could act as a predisposing factor to human neural tube defects PMID: 21901791
MINK1 interacts with and phosphorylates PRICKLE1 and PRICKLE2. PMID: 22037766
Mutations in prickle1 causes seizures. PMID: 21276947
PRICKLE1 mutations are not a frequent cause of progressive myoclous epilepsies in Southern Italy. PMID: 20842693
PRICKLE1 and PRICKLE2 mRNAs were expressed together in brain, eye and testis. PMID: 12525887
appears to serve as a nuclear receptor for REST/NRSF, REST4, and possibly other transcription factors PMID: 14645515
NRSF/REST functions as a repressor of TH transcription in NSCs via a mechanism dependent on the TH NRSE/RE1 sites. PMID: 16764822
Prickle-1 is a negative regulator of the Wnt/beta-catenin signaling pathway and is a putative tumor suppressor in human hepatocellular carcinoma PMID: 17030191
there is synergism between REST and NFkappaB in the suppression of TAC1 in non-neuronal cells PMID: 17709376
A homozygous mutation in PRICKLE1 causes an autosomal-recessive progressive myoclonus epilepsy-ataxia syndrome. PMID: 18976727
A physical and functional interaction between Prickle and Strabismus was found in Drosophila and Xenopus, but is likely conserved in human, too. PMID: 12941693

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Involvement in disease

Epilepsy, progressive myoclonic 1B (EPM1B); Neural tube defects (NTD)

Subcellular Location

Nucleus membrane. Cytoplasm, cytosol. Note=A smaller amount is detected in the cytosol.

Protein Families

Prickle / espinas / testin family

Tissue Specificity

Expressed at highest levels in placenta and at lower levels in lung, liver, kidney and pancreas. Expressed in thalamus, hippocampus, cerebral cortex, and cerebellum (in neurons rather than glia).

Database Links

HGNC: 17019

OMIM: 182940

KEGG: hsa:144165

STRING: 9606.ENSP00000345064

UniGene: Hs.524348

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Recombinant Human Prickle-like protein 1 (PRICKLE1), partial

Product Details

Customer Reviews and Q&A

Target Background

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