Recombinant Human Probable ATP-dependent RNA helicase DDX46 (DDX46), partial

Code CSB-YP748717HU
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Source Yeast
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Code CSB-EP748717HU
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Source E.coli
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Code CSB-EP748717HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP748717HU
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Source Baculovirus
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Code CSB-MP748717HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DDX46
Uniprot No.
Alternative Names
DDX46; KIAA0801Probable ATP-dependent RNA helicase DDX46; EC 3.6.4.13; DEAD box protein 46; PRP5 homolog
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Plays an essential role in splicing, either prior to, or during splicing A complex formation.
Gene References into Functions
  1. Knockdown of DDX46 inhibited osteosarcoma cell proliferation. PMID: 27697093
  2. DDX46 is critical for ESCC cells proliferation. PMID: 27176873
  3. DDX46 is critical for colorectal cancer (CRC) cell proliferation and is a potential therapeutic target for CRC treatment. PMID: 25680556
  4. Short-term exercise resulted in a significant increase of mRNA expression of genes encoding proteins involved in the formation of precatalytic splisosome: DDX46. PMID: 19902070
  5. characterization of protein associated with the human 17S U2 snRNP and one of its stable subunits, SF3b; results indicate that Prp5 plays an important role in pre-mRNA splicing, acting during or prior to prespliceosome assembly PMID: 12234937
  6. Prp5 forms a bridge between U1 and U2 Small Nuclear Ribonucleoproteins at the time of pre-spliceosome formation PMID: 14713954

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Subcellular Location
Nucleus speckle. Nucleus, Cajal body. Membrane; Lipid-anchor. Note=Present in Cajal bodies (CBs) and nuclear speckles.
Protein Families
DEAD box helicase family, DDX46/PRP5 subfamily
Database Links

HGNC: 18681

OMIM: 617848

KEGG: hsa:9879

STRING: 9606.ENSP00000346236

UniGene: Hs.406549

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