Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

PIBF1

Uniprot No.

Q8WXW3

Research Area

Cell Biology

Alternative Names

PIBF1; C13orf24; PIBF; Progesterone-induced-blocking factor 1; Centrosomal protein of 90 kDa; CEP90

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-757aa

Target Protein Sequence

MSRKISKESKKVNISSSLESEDISLETTVPTDDISSSEEREGKVRITRQLIERKELLHNIQLLKIELSQKTMMIDNLKVDYLTKIEELEEKLNDALHQKQLLTLRLDNQLAFQQKDASKYQELMKQEMETILLRQKQLEETNLQLREKAGDVRRNLRDFELTEEQYIKLKAFPEDQLSIPEYVSVRFYELVNPLRKEICELQVKKNILAEELSTNKNQLKQLTETYEEDRKNYSEVQIRCQRLALELADTKQLIQQGDYRQENYDKVKSERDALEQEVIELRRKHEILEASHMIQTKERSELSKEVVTLEQTVTLLQKDKEYLNRQNMELSVRCAHEEDRLERLQAQLEESKKAREEMYEKYVASRDHYKTEYENKLHDELEQIRLKTNQEIDQLRNASREMYERENRNLREARDNAVAEKERAVMAEKDALEKHDQLLDRYRELQLSTESKVTEFLHQSKLKSFESERVQLLQEETARNLTQCQLECEKYQKKLEVLTKEFYSLQASSEKRITELQAQNSEHQARLDIYEKLEKELDEIIMQTAEIENEDEAERVLFSYGYGANVPTTAKRRLKQSVHLARRVLQLEKQNSLILKDLEHRKDQVTQLSQELDRANSLLNQTQQPYRYLIESVRQRDSKIDSLTESIAQLEKDVSNLNKEKSALLQTKNQMALDLEQLLNHREELAAMKQILVKMHSKHSENSLLLTKTEPKHVTENQKSKTLNVPKEHEDNIFTPKPTLFTKKEAPEWSKKQKMKT
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

97.2 kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant Human PIBF1 was expressed with the amino acid range of 1-757. This PIBF1 protein is theoretically predicted to have a molecular weight of 97.2 kDa. The PIBF1 protein was expressed in e.coli. The N-terminal 10xHis tag and C-terminal Myc tag was fused into the coding gene segment of PIBF1, making it easier to detect and purify the PIBF1 recombinant protein in the later stages of expression and purification.

Human progesterone-induced-blocking factor 1 (PIBF1) plays a pivotal role in immune modulation during pregnancy. Its main function involves promoting immune tolerance at the maternal-fetal interface, protecting the developing embryo from rejection. In reproductive and immunological research, studying PIBF1 provides crucial insights into maternal immune adaptation and fetal protection mechanisms. PIBF1's involvement in reproductive disorders and immune-related conditions suggests potential applications in fertility treatments and autoimmune disease research. Investigating PIBF1 contributes to a comprehensive understanding of immune regulation during pregnancy and opens avenues for therapeutic strategies in reproductive health and immunology, addressing challenges in maternal-fetal tolerance and autoimmune disorders.

Target Background

Function

Plays a role in ciliogenesis.; Pericentriolar protein required to maintain mitotic spindle pole integrity. Required for the centrosomal accumulation of PCM1 and the recruitment of centriolar satellite proteins such as BBS4. Via association with PCM1 may be involved in primary cilia formation. Required for CEP63 centrosomal localization and its interaction with WDR62. Together with CEP63 promotes centriole duplication. Promotes the centrosomal localization of CDK2.; The secreted form is a mediator of progesterone that by acting on the phospholipase A2 enzyme interferes with arachidonic acid metabolism, induces a Th2 biased immune response, and by controlling decidual naturakl killer cells (NK) activity exerts an anti-abortive effect. Increases the production of Th2-type cytokines by signaling via the JAK/STAT pathway. Activates STAT6 and inhibits STAT4 phosphorylation. Signaling via a not identified receptor seems to implicate IL4R and a GPI-anchored protein.

Gene References into Functions

We found at high levels of expression of PIBF protein in epithelial ovarian cancer patients. These data demonstrate that tumor cells can secrete PIBF to escape the immune system PMID: 29962287
blood and placental tissue level expression of PIBF is concordant and correlates with both progesterone receptor expression status as well as final pregnancy outcome PMID: 27728856
this study shows that preterm birth may be predictable at 24-28 gestational week by lower than normal pregnancy PIBF values PMID: 27479613
B cells are a significant producer of PIBF1 in human choriodecidua in late gestation. Preterm labor was associated with a lower level of active PIBF1 in late gestation choriodecidua. PMID: 27918564
PIBF plays a role in both normal pregnancy and tumor biology. PMID: 20367622
PIBF promotes proliferation, migration, and invasion of human glioblastoma cells. PMID: 28168193
These data show that exposure to an allogeneic stimulus is not needed to cause a marked rise in PIBF, merely progesterone alone is sufficient. PMID: 26634256
Maternal serum progesterone-induced blocking factor (PIBF) in the prediction of preterm birth. PMID: 25818991
progesterone-induced blocking factor is intracellularly expressed by the cultured cells from Glioblastoma PMID: 24474429
Progesterone-induced blocking factor is hormonally regulated in human astrocytoma cells, and increases their growth through the IL-4R/JAK1/STAT6 pathway PMID: 25218441
decreased kisspeptin and PIBF expressions in trophoblasts and deciduas are associated with recurrent spontaneous abortion PMID: 24225150
reduced expression during late pregnancy and resorption PMID: 24325791
Data indicate that silencing of progesterone-induced blocking factor (PIBF) increased invasiveness as well as MMP-2,-9 secretion of HTR8/SVneo trophoblast, and decreased those of HT-1080 cells. PMID: 23807209
CEP90 physically interacts with PCM-1 at centriolar satellites, and this interaction is essential for centrosomal accumulation of the centriolar satellites and eventually for primary cilia formation. PMID: 23110211
Suggest that urinary PIBF levels may predict graft rejection in transplant recipients. PMID: 22172828
increased serum levels after dydrogesterone supplementation in women with threatened preterm delivery; positive correlation with the length of gestation PMID: 22032897
CEP90 was characterized as a component of pericentriolar satellites. PMID: 21224392
PIBF affects the expression of leptin and its receptor, and PIBF expression is inversely related to trophoblast invasiveness. PMID: 21632119
promotes secretion of type 2 cytkines by activated lymphocytes from pregnant woman PMID: 19371956
The 48-kDa N-terminal part of PIBF is biologically active, and the part of the molecule responsible for modulating natural killer cell activity is encoded by exons 2-4. PMID: 14634107
PIBF production is a characteristic feature of normal pregnancy, and determination of PIBF concentration in urine might be of use for the diagnosis of threatened premature pregnancy termination. PMID: 15269099
progesterone induced blocking factor overexpression is associated with breast neoplasms PMID: 15305375
PIBF phosphorylates PKC via binding to the IL-4R, without affecting intracellular Ca(++). These findings explain the mechanism by which PIBF supports a Th2 dominant cytokine pattern. PMID: 16433831
PIBF concentrations in urine and serum of patients with threatened abortion were significantly lower than in healthy pregnant women. PMID: 19290853

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Involvement in disease

May be associated with microcephaly.

Subcellular Location

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm. Secreted.; [Isoform 1]: Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite. Secreted.; [Isoform 4]: Secreted.

Tissue Specificity

Expressed at highest levels in testis. Moderate expression is detected in spleen, thymus, prostate, ovary, small intestine, and colon. Expressed in the first trimester pregnancy decidua. Localized to extravillous cytotrophoblast (at protein level). Also f

Database Links

HGNC: 23352

OMIM: 607532

KEGG: hsa:10464

STRING: 9606.ENSP00000317144

UniGene: Hs.441926

Code	CSB-EP845171HU
Abbreviation	Recombinant Human PIBF1 protein
MSDS	MSDS Datasheet
Size	US$306
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Image	Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP845171HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) PIBF1. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP845171HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) PIBF1.
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Recombinant Human Progesterone-induced-blocking factor 1 (PIBF1)

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