Recombinant Human Proline/serine-rich coiled-coil protein 1 (PSRC1)

Product Details

Purity

≥85% (SDS-PAGE)

Target Names

PSRC1

Uniprot No.

Alternative Names

DDA3; DDA3; mouse; homolog of; Differential display and activated by p53; FP3214; OTTHUMP00000013785; OTTHUMP00000013786; p53-regulated DDA3; Proline/serine-rich coiled-coil 1; Proline/serine-rich coiled-coil protein 1; PSRC1; PSRC1_HUMAN

Species

Homo sapiens (Human)

Expression Region

1-363

Target Protein Sequence

MEDLEEDVRF IVDETLDFGG LSPSDSREEE DITVLVTPEK PLRRGLSHRS DPNAVAPAPQ GVRLSLGPLS PEKLEEILDE ANRLAAQLEQ CALQDRESAG EGLGPRRVKP SPRRETFVLK DSPVRDLLPT VNSLTRSTPS PSSLTPRLRS NDRKGSVRAL RATSGKRPSN MKRESPTCNL FPASKSPASS PLTRSTPPVR GRAGPSGRAA ASEETRAAKL RVSGSGEFVG LTLKFLHPSP PGPPTPIRSV LAPQPSTSNS QRLPRPQGAA AKSSSQLPIP SAIPRPASRM PLTSRSVPPG RGALPPDSLS TRKGLPRPST AGHRVRESGH KVPVSQRLNL PVMGATRSNL QPPRKVAVPG PTR

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Required for normal progression through mitosis. Required for normal congress of chromosomes at the metaphase plate, and for normal rate of chromosomal segregation during anaphase. Plays a role in the regulation of mitotic spindle dynamics. Increases the rate of turnover of microtubules on metaphase spindles, and contributes to the generation of normal tension across sister kinetochores. Recruits KIF2A and ANKRD53 to the mitotic spindle and spindle poles. May participate in p53/TP53-regulated growth suppression.

Gene References into Functions

these data indicate that ASB7 plays a crucial role in regulating spindle dynamics and genome integrity by controlling the expression of DDA3. PMID: 27697924
no association was found between the SNPs of rs599839, rs464218 and rs6698843 at the CELSR2-PSRC1-SORT1 and the risk of coronary artery disease or ischemic stroke PMID: 26464717
Thus, our findings identified a definite regulatory mechanism of the search and capture process for stable spindle attachment through cross-talk between spindle dynamics and KT composition mediated by DDA3 and Ska1. PMID: 26797278
PSRC1 in the cholesterol gene cluster shows a significant association with coronary artery disease and its single nucleotide polymorphism regulates plasma cholesterol levels. PMID: 24674750
Thus, the EB1-based function of DDA3 links MT dynamics to directional cell migration PMID: 23652583
kinases control the function of DDA3 in the cell cycle by regulating its microtubules-polymerizing/bundling activities through sequential phosphorylation. PMID: 21473853
MCAK and CENP-E are involved in DDA3-mediated chromosome congression. PMID: 21426902
the mitotic function of DDA3 is regulated by phosphorylation on the Ser225 residue. PMID: 20117088
The C-terminal domain confers its ability to associate with the mitotic spindle, while the regulatory N-terminal domain controls the microtubule-binding by the C-terminal domain and determines the cellular activity of the DDA3 protein. PMID: 19738423
molecular cloning, sequence analysis and gene expression PMID: 12427559
Together our results show that hDDA3 is a p53- and DNA-damage down-regulated target that exhibits oncogenic characteristics. PMID: 18291097
DDA3 represents a new class of microtubule-destabilizing protein that controls spindle dynamics and mitotic progression by regulating microtubule depolymerases. PMID: 18411309
The novel CAD-associated locus in the vicinity of the PSRC1 and CELSR2 genes on chromosome 1 probably enhances CAD risk through an effect on plasma LDL cholesterol. PMID: 18649068
Together these results identify ASPP2 as a bona fide DDA3 interacting protein, and suggest that the ASPP2/DDA3 interaction may inhibit ASPP2 in stimulating the apoptotic signaling of p53. PMID: 18793611

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Subcellular Location

Cytoplasm. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Note=Detected at the mitotic spindle and spindle poles. Diffusely distributed throughout the cell during interphase.

Protein Families

PSRC1 family

Tissue Specificity

Widely expressed in adult and fetal tissues, with highest expression in the adult brain and fetal thymus. Not detected in adult skeletal muscle.

Database Links

HGNC: 24472

OMIM: 613126

KEGG: hsa:84722

UniGene: Hs.405925

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Recombinant Human Proline/serine-rich coiled-coil protein 1 (PSRC1)

Product Details

Customer Reviews and Q&A

Target Background

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