Recombinant Human Prostaglandin E synthase 3 (PTGES3) is produced through a yeast expression system, which appears to deliver high-quality protein expression. This full-length protein covers amino acids 1 to 160 and includes an N-terminal 10xHis-tag that helps with purification and detection. The product shows purity greater than 85% when measured by SDS-PAGE, suggesting it may be suitable for various research applications. However, it's only available for research use, with no claims for diagnostic or therapeutic purposes.
Prostaglandin E synthase 3 (PTGES3) is an enzyme that participates in prostaglandin biosynthesis. Prostaglandins are lipid compounds that play critical roles in inflammation and other physiological processes. PTGES3 seems particularly important for converting prostaglandin H2 to prostaglandin E2 - a molecule with significant implications for immune response regulation and other biological pathways. Understanding this enzyme is likely crucial for grasping how these key processes work in human health and disease.
Potential Applications
Note: The applications listed below are based on what we know about this protein's biological functions, published research, and experience from experts in the field. However, we haven't fully tested all of these applications ourselves yet. We'd recommend running some preliminary tests first to make sure they work for your specific research goals.
1. Protein-Protein Interaction Studies
The N-terminal 10xHis-tagged PTGES3 works well in pull-down assays for identifying and characterizing protein binding partners involved in prostaglandin E2 biosynthesis pathways. The His-tag allows efficient attachment to nickel-affinity resins during co-immunoprecipitation experiments with cell lysates or purified protein libraries. This strategy might help clarify the molecular mechanisms behind PTGES3 function and its role in cellular signaling networks. Since the protein includes the complete sequence (1-160aa), all potential binding domains remain intact for interaction studies.
2. Antibody Development and Validation
This recombinant PTGES3 protein works as an effective antigen for creating specific antibodies against human PTGES3 in research settings. The high purity (>85%) and complete sequence make it appropriate for immunizing animals to produce polyclonal antibodies or screening monoclonal antibody clones. The His-tagged protein also proves useful for validating antibody specificity through ELISA-based assays and Western blot controls. Scientists can apply this protein to establish reliable detection methods for PTGES3 across different experimental systems.
3. Biochemical Characterization and Enzyme Assays
The purified PTGES3 protein enables biochemical studies investigating its structural properties and potential enzymatic activities related to prostaglandin metabolism. Scientists can conduct thermal stability assays, protein folding studies, and cofactor binding experiments to better understand the protein's biochemical characteristics. The yeast expression system likely provides properly folded eukaryotic protein that maintains native-like conformational features essential for functional studies.
4. His-Tag Affinity Purification Method Development
This His-tagged PTGES3 protein can function as a model system for refining purification protocols and developing standardized methods for isolating PTGES3 from various expression systems. Scientists may use this protein to test different buffer conditions, elution strategies, and chromatographic approaches for maximum yield and purity. The established purification methods can then be applied to isolate endogenous or recombinant PTGES3 from other biological sources for comparative studies.
