Purity
Greater than 90% as determined by SDS-PAGE.
Species
Homo sapiens (Human)
Expression Region
33-382aa
Target Protein Sequence
KELKFVTLVFRHGDRSPIDTFPTDPIKESSWPQGFGQLTQLGMEQHYELGEYIRKRYRKFLNESYKHEQVYIRSTDVDRTLMSAMTNLAALFPPEGVSIWNPILLWQPIPVHTVPLSEDQLLYLPFRNCPRFQELESETLKSEEFQKRLHPYKDFIATLGKLSGLHGQDLFGIWSKVYDPLYCESVHNFTLPSWATEDTMTKLRELSELSLLSLYGIHKQKEKSRLQGGVLVNEILNHMKRATQIPSYKKLIMYSAHDTTVSGLQMALDVYNGLLPPYASCHLTELYFEKGEYFVEMYYRNETQHEPYPLMLPGCSPSCPLERFAELVGPVIPQDWSTECMTTNSHQVLK
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Partial of Isoform 2
Tag Info
C-terminal 10xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
To produce the recombinant human prostatic acid phosphatase (ACP3) in yeast, the gene fragment encoding the specific region of interest, amino acids 33-382 of human ACP3, is fused with a C-terminal 10xHis-tag and inserted into a specialized expression vector designed for yeast systems. This expression vector contains yeast-specific regulatory elements, including a promoter and terminator, to drive efficient expression of the recombinant protein. The constructed expression vector is then introduced into yeast host cells via a transformation process. The transformed cells that incorporate the recombinant plasmid DNA into their genome are selected using a selectable marker. The selected yeast cells are cultivated in a suitable growth medium that supports their growth and protein expression. Inside the yeast cells, the recombinant ACP3 protein is synthesized using the cellular machinery of the host. Once synthesis is complete, the yeast cells are lysed, releasing the intracellular contents, including the expressed recombinant human ACP3 protein. The purified recombinant ACP3 protein is obtained from the cell lysate, and its purity is assessed using SDS-PAGE analysis. The protein demonstrates a purity level of over 90% and appears as a distinct band with an approximate molecular weight of 46 kDa on the gel.