Product Details

Uniprot No.

Target Names

ACPP

Alternative Names

Prostatic acid phosphatase (PAP) (EC 3.1.3.2) (5'-nucleotidase) (5'-NT) (EC 3.1.3.5) (Ecto-5'-nucleotidase) (Thiamine monophosphatase) (TMPase) [Cleaved into: PAPf39], ACPP

Species Reactivity

Human, Mouse

Immunogen

Recombinant Antibody

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8A7

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Prostatic acid phosphatase, encoded by the ACPP gene, serves as a well-established biomarker in prostate cancer research and clinical diagnostics. This secreted glycoprotein functions as both an acid phosphatase and 5'-nucleotidase, playing roles in cellular metabolism and signaling pathways relevant to prostate tissue biology. Its elevated expression in prostate carcinoma has made it a valuable target for researchers investigating disease progression, tissue characterization, and therapeutic development.

This recombinant monoclonal antibody, clone 8A7, offers the consistency and reliability that demanding experimental workflows require. Because it is produced from a defined sequence in a controlled expression system, researchers can expect reproducible performance across experiments and over time, eliminating the lot-to-lot variability that can complicate long-term studies or multi-site collaborations. The rabbit IgG format, purified by affinity chromatography, ensures high specificity for human ACPP.

Validation studies demonstrate robust performance across multiple detection platforms. Western blot analysis confirms specific detection at the expected 45 kDa molecular weight in HEK293 and HEK293T whole cell lysates, with clean band visualization at 1:1000 dilution. For tissue-based studies, immunohistochemistry validation in paraffin-embedded human prostate cancer sections shows clear staining at dilutions between 1:50 and 1:200, with optimized protocols using citrate buffer antigen retrieval and standard polymer detection systems. The antibody is also validated for ELISA applications, providing flexibility for quantitative analyses.

This antibody supports researchers working in prostate cancer biology, urological pathology, and biomarker development, offering a dependable tool for both discovery research and translational applications where consistent target detection is essential.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma.; Tyrosine phosphatase that acts as a tumor suppressor of prostate cancer through dephosphorylation of ERBB2 and deactivation of MAPK-mediated signaling. In addition to its tyrosine phosphatase activity has ecto-5'-nucleotidase activity in dorsal root ganglion (DRG) neurons. Generates adenosine from AMP which acts as a pain suppressor.; (Microbial infection) Forms amyloid beta-sheet fibrils in semen. These fibrils, termed SEVI (semen-derived enhancer of viral infection) capture HIV virions, attach them to target cells and enhance infection. SEVI amyloid fibrils are degraded by polyphenol epigallocatechin-3-gallate (EGCG), a constituent of green tea. Target cell attachment and enhancement of HIV infection is inhibited by surfen. Also similarly boosts XMRV (xenotropic murine leukemia virus-related virus) infection.

Gene References into Functions

Enhanced TDPase and TMPase activities may contribute to the reduction of TDP level in AD patients. The results imply that an imbalance of phosphorylation-dephosphorylation related to thiamine and glucose metabolism may be a potential target for AD prevention and therapy. PMID: 28060825
Thirteen single nucleotide polymorphism (SNPs) in acid phosphatase prostate (ACPP) were suggested as candidate causal alleles that underlie ACPP regulation and expression. PMID: 27357282
we have measured the intramolecular diffusion of the full length and 8-residue deletion peptides at two different pHs and found a correlation with fibrillization lag time. These results can be explained by a simple kinetic model of the early stages of aggregation in which oligomerization is controlled by the rate of peptide reconfiguration. PMID: 27393931
Prostatic acid phosphatase delays prostate cancer cell growth in G1 phase of the cell cycle. PMID: 26419820
Studies suggest that understanding of prostatic acid phosphatase function and regulation of expression will have a significant impact on understanding prostate cancer (PCa) progression and therapy. PMID: 23698773
Certain factors identified within semen, termed semen-derived enhancers of virus infection (SEVI), fragments of prostatic acid phosphatase, have been shown to significantly enhance HIV-1 infectivity. PMID: 25740984
ACPP increases significantly in epithelial cells of ovarian carcinoma, which indicates that it may be a candidate biomarker for diagnosis of epithelia-derived ovarian cancer in women. PMID: 24829029
Data indicate that hypoxia regulates prostatic acid phosphatase (PAP) through hypoxia-inducible factor 2 alpha (HIF2alpha) and from stimulated A2B adenosine receptors. PMID: 24434023
GCNT1 is over-expressed in prostate cancer and is associated with higher levels of core 2 O-sLe(x) in PSA, PAP and MUC1 proteins. PMID: 24854630
Data indicate that prostate acid phosphatase-based peptide vaccine PAP-114-128 peptide appears to be a relevant for the treatment of prostate cancer. PMID: 24338683
Soluble ecto-5'-nucleotidase (5'-NT), alkaline phosphatase, and adenosine deaminase (ADA1) activities in neonatal blood favor elevated extracellular adenosine. PMID: 23897810
PAPf39 is a 39 residue peptide fragment from human prostatic acidic phosphatase. Recombinant PAPf39 showed amyloid fibril formation. PMID: 23314347
in the in vivo environment, PAP(248-286) is likely to form fibrils efficiently, thus providing an explanation for the presence of semen-derived enhancer of viral infection in human semen. PMID: 22354963
Peptide fragments derived from N-proximal and C-proximal of the PAP form fibrillar structures and increase virion attachment to cells. PMID: 22090109
PAP is strongly expressed in prostate cancer bone metastases in 7/7 patients, while prostate-specific antigen (PSA) is only weakly expressed. PMID: 22082367
Our studies confirmed that, while prostatic acid phosphatase expression is not restricted to prostate tissues, its expression in other human tissues is approximately 1-2 orders of magnitude less than that observed in the prostate. PMID: 21487525
Most of the features of PAP including gene regulation, gene/protein structure, functions, its role in tumor progression and evolutionary features are discussed. Review. PMID: 20645695
prostatic acid phosphatase, an authentic tyrosine phosphatase, dephosphorylates ErbB-2 and regulates prostate cancer cell growth PMID: 20498373
Data show that prostatic acid phosphatase(PAP) as a sensitive tumor marker for prostate cancer. PMID: 20392611
PSAP might be predictive of tumor stage in incidental prostate cancer and represent a valuable adjunct for clinical decisions in terms of individual therapeutic management. PMID: 19301031
The obtained N-terminal amino-acid sequence of boar PTAP showed 92% identity with the N-terminal amino-acid sequence of human PAP. The determined sequence of a 354 bp nucleotide fragment showed 90% identity with the corresponding sequence of human PAP. PMID: 19759923
Effect of tartaric acid on conformation and stability of human prostatic phosphatase: an infrared spectroscopic and calorimetric study. PMID: 11833784
Identification and characterization of regulatory elements of the human prostatic acid phosphatase promoter. PMID: 12032838
analysis of mRNA levels in hyperplastic prostate stimulated with steroid hormones and growth factors PMID: 12362977
equilibrium unfolding of dimeric human prostatic acid phosphatase involves an inactive monomeric intermediate PMID: 12719131
In dilute solutions, several active prostatic acid phosphatase species exist, which are involved in concentration-dependent dissociation/association equilibria PMID: 12962324
Human prostatic acid phosphatase's regulatory regions were analyzed in transgenic mice and cell line transfections, in order to clarify the mechanisms of tissue-specific gene expression PMID: 14623260
protein binding with Con A in seminal plasma PMID: 14690244
Transcriptional activation of the prostatic acid phosphatase gene by NF-kappaB in human prostate cancer cells. PMID: 15240830
Prostatic acid phosphatase inactivates lysophosphatidic acid in seminal plasma. PMID: 15280042
analysis of prostatic acid phosphatase binding PMID: 15578709
the GAAAATATGATA-like elements are involved in the transcriptional regulation of hPAP promoter constructs in prostatic cells. PMID: 15985366
JFC1 differentially regulates the secretion of PSAP and PSA, and Rab27a and PI3K play a central role in the exocytosis of prostate-specific markers. PMID: 16004602
1,25D-mediated decreases in prostate cancer cells and C81 LN cell growth are in part due to decreases in tyrosine kinase signaling that result from up-regulation of cellular prostatic acid phosphatase. PMID: 16076555
PAP (133-152) and PAP (173-192) were immunogenic and processed from whole PAP in HLA-DRB1*1501 tg mice. These peptides were capable of stimulating CD4 T lymphocytes from HLA-DRB1*1501-positive patients with granulomatous prostatitis and normal donors. PMID: 17455230
Prostatic acid phosphatase is not a prostate specific target PMID: 17638863
Suppresses pain by functioning as an ecto-5'-nucleotidase, activating A1-adenosine receptors in the dorsal spinal cord. PMID: 18940592
presence of Prostatic Acid Phosphatase in breast cyst fluid may suggest its possible role in the development of breast cancer from cystic breast diseases PMID: 19108401
Prostatic acid phosphatase boosts the infectivity of xenotropic murine leukemia virus-related virus. PMID: 19403677
Report safety and immunological efficacy of a DNA vaccine encoding prostatic acid phosphatase in patients with stage D0 prostate cancer. PMID: 19636017
molecular details of PAPf39 peptide fibril formation may aid in elucidating the mechanism of how PAPf39 fibrils are involved in HIV etiology PMID: 19902966

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Subcellular Location

[Isoform 1]: Secreted.; [Isoform 2]: Cell membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Nucleus. Cytoplasm, cytosol.

Protein Families

Histidine acid phosphatase family

Tissue Specificity

Highly expressed in the prostate, restricted to glandular and ductal epithelial cells. Also expressed in bladder, kidney, pancreas, lung, cervix, testis and ovary. Weak expression in a subset of pancreatic islet cells, squamous epithelia, the pilosebaceou

Database Links

HGNC: 125

OMIM: 171790

KEGG: hsa:55

STRING: 9606.ENSP00000323036

UniGene: Hs.433060

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Code	CSB-RA255091A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: HEK293 whole cell lysate(30µg), HEK293T whole cell lysate(30µg), Mouse liver tissue lysate(30µg) All lanes: PAP antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time：3min IHC image of CSB-RA255091A0HU diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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ACPP Recombinant Monoclonal Antibody

Product Details

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Customer Reviews and Q&A

Target Background