Recombinant Human Protamine-2 (PRM2)

Product Details

Purity

≥85% (SDS-PAGE)

Target Names

PRM2

Uniprot No.

Alternative Names

PRM2; Protamine-2; Sperm histone P2; Sperm protamine P2) [Cleaved into: Basic nuclear protein HPI1; Basic nuclear protein HPI2; Basic nuclear protein HPS1; Basic nuclear protein HPS2; Sperm histone HP4; Sperm protamine P4); Sperm histone HP2; Sperm protamine P2; P2'); Sperm histone HP3; P2''; Sperm protamine P3)]

Species

Homo sapiens (Human)

Expression Region

2-102

Target Protein Sequence

VRYRVRSLS ERSHEVYRQQ LHGQEQGHHG QEEQGLSPEH VEVYERTHGQ SHYRRRHCSR RRLHRIHRRQ HRSCRRRKRR SCRHRRRHRR GCRTRKRTCR RH

Protein Length

Full Length of Mature Protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Protamines substitute for histones in the chromatin of sperm during the haploid phase of spermatogenesis. They compact sperm DNA into a highly condensed, stable and inactive complex.

Gene References into Functions

Authors examined, for the first time in a Turkish population, the association between protamine gene alleles (PRM1 c.-190C>A, PRM1 c.197G>T, and PRM2 c.248C>T), and YBX2 (c.187T>C and c.1095 + 16A>G) and male infertility. PMID: 29227750
PRM2 gene expression was significantly decreased in immature sperm extracted from the fertile and infertile groups. Caspase 9 activity was significantly increased in immature sperm extracted from both groups. PMID: 26392304
The expressed human protamine2 in two fast-growing cell systems, E. coli and HeLa cells. The protamine2 expression significantly attenuated cell proliferation when compared with control cells. PMID: 25649960
Data indicate changed ecpression levels of PRM2, HSP90 and WNT5A in male of couples experiencing idiopathic recurrent miscarriages. PMID: 23271023
Single nuclotide polymorphisms in PRM2 gene is associated with male infertility. PMID: 22104739
analyse the presence of DAZ, RBMY1, USP9Y, protamine-2, SRY and actin messenger RNA (mRNA) in testicular cells of men suffering from idiopathic azoospermia. PMID: 11869379
significantly enhanced evolutionary rates in the hominid lineage PMID: 12524352
Altered protamine P1/P2 ratio effects fertilization rate and embryo quality which subsequently may affect implantation and pregnancy outcome. PMID: 15670415
levels of pre-P2 may provide clues into pathogenesis of infertility. increased proportion of pre-P2 in some men with increased P1/P2 ratio suggests involvement of pre-P2 processing & link between deficient protamine processing & decreased DNA integrity. PMID: 16632464
Novel polycythemia vera (PV)-associated tumor antigen PV13 (protamine 2) elicits IgG antibody reactions in a subset of PV patients but not in healthy donors, suggesting that it is an authentic tumor antigens. PMID: 17113348
The PRM2 contributes not only to successful fertilization, but may has an important impact in development of preimplantation embryos. PMID: 18292840
A reduction in contents of PRM2 transcript in spermatozoa may be linked with asthenozoospermia. PMID: 18292846

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Subcellular Location

Nucleus. Chromosome.

Protein Families

Protamine P2 family

Tissue Specificity

Testis.

Database Links

HGNC: 9448

OMIM: 182882

KEGG: hsa:5620

STRING: 9606.ENSP00000241808

UniGene: Hs.2324

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Recombinant Human Protamine-2 (PRM2)

Product Details

Customer Reviews and Q&A

Target Background

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