Recombinant Human Protein-arginine deiminase type-1 (PADI1)

Code CSB-BP891543HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-EP891543HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-MP891543HU
MSDS
Size Pls inquire
Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PADI1
Uniprot No.
Alternative Names
HPAD10; Padi1; PADI1_HUMAN; PDI; PDI1; Peptidylarginine deiminase I; protein arginine deiminase type I; Protein-arginine deiminase type I; Protein-arginine deiminase type-1
Species
Homo sapiens (Human)
Expression Region
1-663
Target Protein Sequence
MAPKRVVQLS LKMPTHAVCV VGVEAHVDIH SDVPKGANSF RVSGSSGVEV FMVYNRTRVK EPIGKARWPL DTDADMVVSV GTASKELKDF KVRVSYFGEQ EDQALGRSVL YLTGVDISLE VDTGRTGKVK RSQGDKKTWR WGPEGYGAIL LVNCDRDNHR SAEPDLTHSW LMSLADLQDM SPMLLSCNGP DKLFDSHKLV LNVPFSDSKR VRVFCARGGN SLSDYKQVLG PQCLSYEVER QPGEQEIKFY VEGLTFPDAD FLGLVSLSVS LVDPGTLPEV TLFTDTVGFR MAPWIMTPNT QPPEELYVCR VMDTHGSNEK FLEDMSYLTL KANCKLTICP QVENRNDRWI QDEMEFGYIE APHKSFPVVF DSPRNRGLKD FPYKRILGPD FGYVTREIPL PGPSSLDSFG NLDVSPPVTV GGTEYPLGRI LIGSSFPKSG GRQMARAVRN FLKAQQVQAP VELYSDWLSV GHVDEFLTFV PTSDQKGFRL LLASPSACLK LFQEKKEEGY GEAAQFDGLK HQAKRSINEM LADRHLQRDN LHAQKCIDWN RNVLKRELGL AESDIVDIPQ LFFLKNFYAE AFFPDMVNMV VLGKYLGIPK PYGPIINGRC CLEEKVQSLL EPLGLHCIFI DDYLSYHELQ GEIHCGTNVR RKPFPFKWWN MVP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalyzes the deimination of arginine residues of proteins.
Gene References into Functions
  1. Authors showed that PAD1 interacts with and citrullinates MEK1 thereby disrupting MEK1-catalyzed ERK1/2 phosphorylation, thus leading to the MMP2 overexpression. PMID: 28844713
  2. Identify acefylline as an activator of peptidylarginine deiminase 1 and 3 in the epidermis, resulting in filaggrin deimination. PMID: 26616205
  3. NF-kappaB-mediated signaling pathway is involved in PADI1 regulation in human epidermal keratinocytes PMID: 20596086
  4. cloning, gene organization, and expression analysis PMID: 12416996
  5. peptidylarginine deiminase types 1 and 3 loci map to chromosomal band 1p36.13 PMID: 15150696
  6. PAD1 and 3 are able to modify filaggrin PMID: 15675958
  7. PAD1 is co-located with filaggrin within the filamentous matrix of the deeper corneocytes where the protein is deiminated. PMID: 16091842
  8. MZF1 and Sp1/Sp3 binding to the promoter region drive the PADI1 expression in keratinocytes PMID: 17851584
  9. The expressoin of COL1A1-PADI1 genes were particularly effective in distinguishing OCSS from normal tissue. PMID: 18669583
  10. peptidylarginine deiminase citrullinates the chemokine CXCL8, and thus may dampen neutrophil extravasation during acute or chronic inflammation PMID: 18710930

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Subcellular Location
Cytoplasm.
Protein Families
Protein arginine deiminase family
Tissue Specificity
Detected in epidermal keratinocytes (at protein level). Epidermis, prostate, testis, placenta, spleen and thymus.
Database Links

HGNC: 18367

OMIM: 607934

KEGG: hsa:29943

STRING: 9606.ENSP00000364620

UniGene: Hs.412941

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