Recombinant Human Protein disulfide-isomerase-like protein of the testis (PDILT)

Code CSB-YP818726HU
MSDS
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Source Yeast
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Code CSB-EP818726HU
MSDS
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Source E.coli
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Code CSB-EP818726HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP818726HU
MSDS
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Source Baculovirus
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Code CSB-MP818726HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PDILT
Uniprot No.
Alternative Names
1700007B13Rik; PDIA7; Pdilt; PDILT_HUMAN; Protein disulfide isomerase family A member 7; Protein disulfide isomerase like testis expressed; Protein disulfide-isomerase-like protein of the testis; RGD1307822
Species
Homo sapiens (Human)
Expression Region
21-584
Target Protein Sequence
SPEVNAGVSS IHITKPVHIL EERSLLVLTP AGLTQMLNQT RFLMVLFHNP SSKQSRNLAE ELGKAVEIMG KGKNGIGFGK VDITIEKELQ QEFGITKAPE LKLFFEGNRS EPISCKGVVE SAALVVWLRR QISQKAFLFN SSEQVAEFVI SRPLVIVGFF QDLEEEVAEL FYDVIKDFPE LTFGVITIGN VIGRFHVTLD SVLVFKKGKI VNRQKLINDS TNKQELNRVI KQHLTDFVIE YNTENKDLIS ELHIMSHMLL FVSKSSESYG IIIQHYKLAS KEFQNKILFI LVDADEPRNG RVFKYFRVTE VDIPSVQILN LSSDARYKMP SDDITYESLK KFGRSFLSKN ATKHQSSEEI PKYWDQGLVK QLVGKNFNVV VFDKEKDVFV MFYAPWSKKC KMLFPLLEEL GRKYQNHSTI IIAKIDVTAN DIQLMYLDRY PFFRLFPSGS QQAVLYKGEH TLKGFSDFLE SHIKTKIEDE DELLSVEQNE VIEEEVLAEE KEVPMMRKGL PEQQSPELEN MTKYVSKLEE PAGKKKTSEE VVVVVAKPKG PPVQKKKPKV KEEL
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Probable redox-inactive chaperone involved in spermatogenesis.
Gene References into Functions
  1. indings have identified the critical regions and conformational changes of PDILT that enable and control its activity. These results advance our understanding of the structural mechanisms involved in the chaperone activity of PDILT. PMID: 29203529
  2. Further studies of this recently discovered PDI member would help to understand the important role that PDILT plays in the differentiation and maturation of spermatozoids. PMID: 24662985
  3. The expression of PDILT, in addition to PDI in the testis, suggests that PDILT performs a specialized chaperone function in testicular cells. PMID: 15475357
Subcellular Location
Endoplasmic reticulum.
Protein Families
Protein disulfide isomerase family
Tissue Specificity
Testis-specific.
Database Links

HGNC: 27338

KEGG: hsa:204474

STRING: 9606.ENSP00000305465

UniGene: Hs.376025

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