Recombinant Human Protein-glutamine gamma-glutamyltransferase 5 (TGM5)

Code CSB-YP023465HU
MSDS
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Source Yeast
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Code CSB-EP023465HU
MSDS
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Source E.coli
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Code CSB-EP023465HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP023465HU
MSDS
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Source Baculovirus
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Code CSB-MP023465HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TGM5
Uniprot No.
Alternative Names
Protein glutamine gamma glutamyltransferase 5; Protein-glutamine gamma-glutamyltransferase 5; PSS2; TG(X); TGase 5; TGase X; TGase-5; TGase5; TGASEX; Tgm5; TGM5_HUMAN; TGM6; TGMX; TGX; Transglutaminase X; Transglutaminase-5
Species
Homo sapiens (Human)
Expression Region
2-720
Target Protein Sequence
AQGLEVALT DLQSSRNNVR HHTEEITVDH LLVRRGQAFN LTLYFRNRSF QPGLDNIIFV VETGPLPDLA LGTRAVFSLA RHHSPSPWIA WLETNGATST EVSLCAPPTA AVGRYLLKIH IDSFQGSVTA YQLGEFILLF NPWCPEDAVY LDSEPQRQEY VMNDYGFIYQ GSKNWIRPCP WNYGQFEDKI IDICLKLLDK SLHFQTDPAT DCALRGSPVY VSRVVCAMIN SNDDNGVLNG NWSENYTDGA NPAEWTGSVA ILKQWNATGC QPVRYGQCWV FAAVMCTVMR CLGIPTRVIT NFDSGHDTDG NLIIDEYYDN TGRILGNKKK DTIWNFHVWN ECWMARKDLP PAYGGWQVLD ATPQEMSNGV YCCGPASVRA IKEGEVDLNY DTPFVFSMVN ADCMSWLVQG GKEQKLHQDT SSVGNFISTK SIQSDERDDI TENYKYEEGS LQERQVFLKA LQKLKARSFH GSQRGAELQP SRPTSLSQDS PRSLHTPSLR PSDVVQVSLK FKLLDPPNMG QDICFVLLAL NMSSQFKDLK VNLSAQSLLH DGSPLSPFWQ DTAFITLSPK EAKTYPCKIS YSQYSQYLST DKLIRISALG EEKSSPEKIL VNKIITLSYP SITINVLGAA VVNQPLSIQV IFSNPLSEQV EDCVLTVEGS GLFKKQQKVF LGVLKPQHQA SIILETVPFK SGQRQIQANM RSNKFKDIKG YRNVYVDFAL
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. Contributes to the formation of the cornified cell envelope of keratinocytes.
Gene References into Functions
  1. Acral PSS (APSS) (OMIM 609796), typically non-inflammatory, is confined to distal extremities with localized exfoliation of the epidermis. It is caused by mutations in the TGM5 gene, encoding transglutaminase 5[3] or in the CTSA gene, encoding cystatin A and it is inherited in autosomal recessive pattern PMID: 25510201
  2. We report both European and non-European families with acral peeling skin syndrome carrying mutations in the TGM5 gene. In 5 patients, we found 3 novel mutations: c.1001+2_1001+3del, c.1171G>A and c.1498C>T. PMID: 25644735
  3. Data trebles the number of TMG5 mutations and provides further evidence that pCly113Cys is a founder mutation in the European population. PMID: 24628291
  4. Genetic variation in the epidermal transglutaminase genes is not associated with atopic dermatitis. PMID: 23189155
  5. TGM5 mutations impact epidermal differentiation in acral peeling skin syndrome. PMID: 22622422
  6. analysis of a recurrent mutation in the TGM5 gene in European patients with acral peeling skin syndrome PMID: 22036214
  7. Acral peeling skin syndrome with TGM5 gene mutations may resemble epidermolysis bullosa simplex in young individuals. PMID: 20164844
  8. transglutaminase 5 contributes, as a secondary effect, to the hyperkeratotic phenotype in ichthyosis (both vulgaris and lamellar) and in psoriasis. PMID: 12230511
  9. Results demonstrate that transglutaminase 5 is able to induce cell death when intracellularly overexpressed. PMID: 15290346
  10. Data show that transglutaminase (TGase) 5 is acetylated at the N-terminal end, is active upon treatment with phorbol acetate, and co-localises with vimentin intermediate filaments. PMID: 15290349
  11. Transglutaminase 5 is expressed during hair follicle homeostasis. PMID: 16117804
  12. A homozygous missense mutation in TGM5 abolishes epidermal TGM5 activity and causes acral peeling skin syndrome. PMID: 16380904
  13. TG5 full-length enzyme has very low enzymatic activity, while the 53-kDa proteolytically processed form is highly active. PMID: 18509357
  14. A missense mutation in TGM5 causes acral peeling skin syndrome in a Tunisian family. PMID: 19440220

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Involvement in disease
Peeling skin syndrome 2 (PSS2)
Subcellular Location
Cytoplasm. Note=Associated with intermediate filaments.
Protein Families
Transglutaminase superfamily, Transglutaminase family
Tissue Specificity
Expressed in foreskin keratinocytes.
Database Links

HGNC: 11781

OMIM: 603805

KEGG: hsa:9333

STRING: 9606.ENSP00000220420

UniGene: Hs.129719

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