Recombinant Human Protein phosphatase 1 regulatory inhibitor subunit 16B (PPP1R16B)

Code CSB-YP842762HU
MSDS
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Source Yeast
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Code CSB-EP842762HU
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Source E.coli
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Code CSB-EP842762HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP842762HU
MSDS
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Source Baculovirus
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Code CSB-MP842762HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PPP1R16B
Uniprot No.
Alternative Names
ANKRD4; Ankyrin repeat domain containing protein 4; ankyrin repeat domain protein 4; Ankyrin repeat domain-containing protein 4; CAAX box protein TIMAP; hTIMAP; KIAA0823; PP16B_HUMAN; PPP1R16B; Protein phosphatase 1 regulatory inhibitor subunit 16B; protein phosphatase 1, regulatory (inhibitor) subunit 16B; TGF beta inhibited membrane associated protein; TGF-beta-inhibited membrane-associated protein; TIMAP
Species
Homo sapiens (Human)
Expression Region
1-564
Target Protein Sequence
MASHVDLLTE LQLLEKVPTL ERLRAAQKRR AQQLKKWAQY EQDLQHRKRK HERKRSTGGR RKKVSFEASV ALLEASLRND AEEVRYFLKN KVSPDLCNED GLTALHQCCI DNFEEIVKLL LSHGANVNAK DNELWTPLHA AATCGHINLV KILVQYGADL LAVNSDGNMP YDLCEDEPTL DVIETCMAYQ GITQEKINEM RVAPEQQMIA DIHCMIAAGQ DLDWIDAQGA TLLHIAGANG YLRAAELLLD HGVRVDVKDW DGWEPLHAAA FWGQMQMAEL LVSHGASLSA RTSMDEMPID LCEEEEFKVL LLELKHKHDV IMKSQLRHKS SLSRRTSSAG SRGKVVRRAS LSDRTNLYRK EYEGEAILWQ RSAAEDQRTS TYNGDIRETR TDQENKDPNP RLEKPVLLSE FPTKIPRGEL DMPVENGLRA PVSAYQYALA NGDVWKVHEV PDYSMAYGNP GVADATPPWS SYKEQSPQTL LELKRQRAAA KLLSHPFLST HLGSSMARTG ESSSEGKAPL IGGRTSPYSS NGTSVYYTVT SGDPPLLKFK APIEEMEEKV HGCC
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Regulator of protein phosphatase 1 (PP1) that acts as a positive regulator of pulmonary endothelial cell (EC) barrier function. Involved in the regulation of the PI3K/AKT signaling pathway, angiogenesis and endothelial cell proliferation. Regulates angiogenesis and endothelial cell proliferation through the control of ECE1 dephosphorylation, trafficking and activity. Protects the endothelial barrier from lipopolysaccharide (LPS)-induced vascular leakage. Involved in the regulation of endothelial cell filopodia extension. May be a downstream target for TGF-beta1 signaling cascade in endothelial cells. Involved in PKA-mediated moesin dephosphorylation which is important in EC barrier protection against thrombin stimulation. Promotes the interaction of PPP1CA with RPSA/LAMR1 and in turn facilitates the dephosphorylation of RPSA/LAMR1. Involved in the dephosphorylation of EEF1A1.
Gene References into Functions
  1. TIMAP is the versatile protein phosphatase 1 regulator in endothelial cells. (Review) PMID: 29140585
  2. NF2 localizes in nucleus when Ser518 is not phosphorylated, while phosphorylated form is present in cytoplasm and plasma membrane. Data suggest that binding of NF2 to TIMAP and EBP50 is critical in nuclear localization of NF2. (NF2 = neurofibromin 2; TIMAP = TGF-beta-inhibited membrane-associated protein; EBP50 = Ezrin-Radixin-Mosein binding phosphoprotein 50) PMID: 27871951
  3. phosphorylation of TIMAP on Ser331 by PKC represents a new mechanism of endothelial barrier regulation, through the inhibition of phospho-ERM dephosphorylation PMID: 27939168
  4. Results indicate that PKC-phosphorylated ECE-1 is a TIMAP-PP1c substrate and this phosphatase complex has an important role in endothelin-1 production of EC through the regulation of ECE-1 activity. PMID: 26806547
  5. ROCK phosphorylated eEF1A1 is a novel substrate for TIMAP-PP1 underlining the complex regulatory role of TIMAP in the endothelium. PMID: 26497934
  6. TIMAP promotes angiogenesis by suppressing PTEN-mediated Akt inhibition in human glomerular endothelial cells. PMID: 25007873
  7. These data confirmed the barrier-protective role of TIMAP PMID: 21907835
  8. Data suggest that double-thiophosphorylation of TIMAP has minor effect on its binding ability to PP1c, but considerably attenuates its inhibitory effect on the activity of PP1c. PMID: 21466834
  9. TIMAP, a novel CAAX box protein regulated by TGF-beta1 and expressed in endothelial cells. PMID: 12055102
  10. involvement of TIMAP in PKA-mediated moesin dephosphorylation and the importance of this dephosphorylation in TIMAP-mediated endothelial cell barrier protection PMID: 18586956

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Subcellular Location
Cell membrane. Cell membrane; Lipid-anchor. Nucleus. Cell projection.
Database Links

HGNC: 15850

OMIM: 613275

KEGG: hsa:26051

STRING: 9606.ENSP00000299824

UniGene: Hs.45719

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