Recombinant Human Proteinase-activated receptor 3 (F2RL2), partial

Code CSB-YP007926HU1
MSDS
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Source Yeast
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Code CSB-EP007926HU1
MSDS
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Source E.coli
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Code CSB-EP007926HU1-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007926HU1
MSDS
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Source Baculovirus
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Code CSB-MP007926HU1
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
F2RL2
Uniprot No.
Alternative Names
F2RL2; PAR3; Proteinase-activated receptor 3; PAR-3; Coagulation factor II receptor-like 2; Thrombin receptor-like 2
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis.
Gene References into Functions
  1. PAR3 is essential in thrombin stimulated insulin secretion. PMID: 26742564
  2. TFRGAP, a PAR-3 mimicking peptide significantly induced the phosphorylation of eNOS-Thr-495 with minimal phosphorylation of eNOS-Ser-1177 with no change in nitric oxide production. PMID: 26729042
  3. These data provide intriguing novel insights into the diversification of functional selectivity of protease signaling achievable by canonical and noncanonical PAR activation, such as the activation of vascular-protective Tie2 by noncanonical PAR3 activation. PMID: 25320242
  4. In pancreatic adenocarcinoma cells, PAR3 knockdown enhances cell adhesion. We propose this is due to increased expression of E-cadherin, leading to a greater adhesion of free-floating cells to cells bound to the surface via integrins, particularly ITGalphav. PMID: 24699825
  5. Elevated expression of PAR-3, but not PAR-4, was detected in the lungs of idiopathic pulmonary fibrosis patients. PMID: 23739922
  6. A unique contributory role for PAR3 in the complex mechanisms underlying Activated protein C cytoprotective effects. PMID: 23788139
  7. PAR-3 receptors interact with thrombin and increase heme oxygenase-1 expression in synovial fibroblasts. PMID: 22541814
  8. Human PAR-3 is regulated post-transcriptionally via the mRNA-stabilizing factor HuR, whereas transcriptional control involves nuclear factor of activated T cells (NFAT). PMID: 21596928
  9. FXa bound in a punctate manner to thrombi under shear, while thrombin and fibrin(ogen) distributed ubiquitously over platelet-fibrin thrombi PMID: 20454680
  10. genetic polymorphism is associated with peripheral arterial disease severity PMID: 20390230
  11. A novel role is identified for PAR3 in thrombin signaling. PMID: 20442298
  12. Down-regulation of connexin and par-3 signals early anaplasia and malignant change in rectal cancer. PMID: 19513616
  13. upregulated thrombin generation and inhibition of fibrinolysis, occurred in one-third of the Stem cell transplant patients associating with the development of Graft versus host disease PMID: 19718071
  14. Lower plasma levels of thrombin-antithrombin complex correlate to higher recanalisation rates after ischaemic stroke PMID: 19806263
  15. PAR-3 is expressed in human SMC and triggers intracellular calcium signaling. PMID: 14515192
  16. expression of PAR3 mRNA and protein in cells undergoing megakaryocytic maturation suggests a developmental role for PAR3; regulation of PAR3 expression appears to be specifically coupled to the protein kinase C system. PMID: 15504554
  17. a Na-independent Cl-/HCO3- exchange mechanism mediates the recovery of pHi from alkalosis in platelets and that thrombin activates this exchanger by a direct regulatory pathway PMID: 15850610
  18. The changes in levels of blood coagulation factor ii in folic acid deficient rats are reversed by treatment with folic acid. PMID: 16046705
  19. F2R genetic variants may influence the natural history of CHD in patients at high risk of cardiovascular events. PMID: 17347481
  20. PAR-3 is able to signal autonomously to induce IL-8 release mediated by ERK1/2 phosphorylation, which contributes actively to inflammatory responses PMID: 18264801
  21. Thrombin-mediated increases in cytosolic [Ca2+] involve different mechanisms in human pulmonary artery smooth muscle and endothelial cells. PMID: 18836030
  22. This study demonstrates that the anticoagulant profile of thrombin often caused by a mutation of the thrombin scaffold finds its likely molecular origin in the stabilization of the inactive E* form. PMID: 19473969
  23. Mechanism of the anticoagulant activity of thrombin mutant W215A/E217A. PMID: 19586901

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Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
G-protein coupled receptor 1 family
Tissue Specificity
Highest expression in the megakaryocytes of the bone marrow, lower in mature megakaryocytes, in platelets and in a variety of other tissues such as heart and gut.
Database Links

HGNC: 3539

OMIM: 601919

KEGG: hsa:2151

STRING: 9606.ENSP00000296641

UniGene: Hs.42502

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