Recombinant Human Putative methyltransferase NSUN7 (NSUN7)

Code CSB-YP822769HU
MSDS
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Source Yeast
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Code CSB-EP822769HU
MSDS
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Source E.coli
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Code CSB-EP822769HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP822769HU
MSDS
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Source Baculovirus
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Code CSB-MP822769HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NSUN7
Uniprot No.
Alternative Names
NSUN7Putative methyltransferase NSUN7; EC 2.1.1.-; NOL1/NOP2/Sun domain family member 7
Species
Homo sapiens (Human)
Expression Region
1-718
Target Protein Sequence
MLNSTGELEF SNEEDPEIIS QLTSLPLSGG KSSAGVPEKT GYPDSVYVMA ANIFQGIRIE KSAQKVLIKY GNEPLRSLSE SEDQSFQRLS YELAFSALKY QDILETILID SCIFPSTTIP DHLSSLIIVM LYDFQDRKFQ TRVLSDNEEP ISEVQEVENL LNSFKIKLAA ALARCRIKHD ALSIYHILPE TVRKQELRAS TLPLYAWINT CKISPEEVYN NLKRRGYNKV KSVLHIDDKV FAVDQHCYDV LIFPSHLKND LINIDLFKDY KLIFQDKSRS LAVHSVKALL NMDDDVLMVN TGSWYTVSHM SILTNNNTSK VFVCGVQSQA KDPDLKTLFT KIGCKNIEIL HEKFINIESK DHRLQKVKVI LLLPRCSGLG VSNPVEFILN EHEDTEFLKD HSQGGISVDK LHVLAQQQYE QLTHAMKFTK AQAVVYCTCS VFPEENEAVV KKALEFQDLG NKGQPYRLSP PVLPLCSLKE IQLSTDKFFR MEPSEITNGC FLSILTRERD PSETVSVNDV LARAAAKGLL DGIELGKSSK REKKKKKSKT SLTKGATTDN GIQMKIAEFL NRETKASANL SETVTKPPLP QKNTAQVGAS SQTRKPNKLA PHPAVPAFVK NTCPSRPRER QTHFLRPRPE DRMVALKPIK IVLPPVFMPF SSPQGIRSRM PTQHLYCRWV APKALVPTCL PTHSLSRKEE KPKDDTPSSL LRPPRRWL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
May have S-adenosyl-L-methionine-dependent methyl-transferase activity.
Gene References into Functions
  1. mutant protein structures of Nsun7 indicated that the amino acid serine was converted to alanine, the structure of the helix, coil and strand was changed, and the protein folding and ligand binding sites were changed in samples from asthenospermic men with a transversion mutation in exon 7, indicating impairment of protein function. Because Nsun7 plays a role in sperm motility, mutation in exon 7 may lead to infertility. PMID: 24384068
  2. Our findings revealed that genetic polymorphisms in exon7 of the NSUN7 gene are not associated with asthenospermia in Chinese Han men. PMID: 26345859
  3. Nsun7 (A11337)-deletion mutation, causes reduction of its protein rate and associated with sperm motility defect in infertile men. PMID: 25702163
Protein Families
Class I-like SAM-binding methyltransferase superfamily, RsmB/NOP family
Database Links

HGNC: 25857

KEGG: hsa:79730

STRING: 9606.ENSP00000371201

UniGene: Hs.479602

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