Recombinant Human Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16 (DHX16), partial

Code CSB-YP006871HU
MSDS
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Source Yeast
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Code CSB-EP006871HU
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Source E.coli
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Code CSB-EP006871HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP006871HU
MSDS
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Source Baculovirus
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Code CSB-MP006871HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DHX16
Uniprot No.
Alternative Names
2410006N22Rik; ATP-dependent RNA helicase #3; ATP-dependent RNA helicase 3; DAQB-47P19.12; DBP 2; DBP2; DDX 16; DDX16; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 16; DEAD/H box 16; DEAH (Asp-Glu-Ala-His) box polypeptide 16; DEAH box protein ; DEAH box protein 16; DEAH-box protein 16; DEAH-box protein; DHX16; DHX16_HUMAN; fa91b12; KIAA0577; mKIAA0577; PRO2014; PRP 8; Prp2; PRP8; PRPF2; Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16; RNA helicase; wu:fa91b12 ; zgc:55590
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Required for pre-mRNA splicing as component of the spliceosome. Contributes to pre-mRNA splicing after spliceosome formation and prior to the first transesterification reaction.
Gene References into Functions
  1. Data show that G patch domain and KOW motifs protein (GPKOW )interacted directly with the DEAH-box protein 16 (DHX16/hPRP2) and with RNA. PMID: 25296192
  2. Results suggest that mutant DHX16/hPRP2 causes a defective spliceosome to retain unspliced gene transcripts in the nuclei. PMID: 20841358
  3. DHX16 is required for human pre-mRNA splicing after the formation of a pre-catalytic spliceosome. PMID: 20423332
Subcellular Location
Nucleus. Nucleus, nucleoplasm.
Protein Families
DEAD box helicase family, DEAH subfamily, DDX16/PRP8 sub-subfamily
Tissue Specificity
Expressed in the spleen, thyroid and testis. Also expressed in the brain and cerebellum.
Database Links

HGNC: 2739

OMIM: 603405

KEGG: hsa:8449

STRING: 9606.ENSP00000365625

UniGene: Hs.485060

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