Recombinant Human R-spondin-4 (RSPO4)

Code CSB-YP644152HU
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP644152HU
MSDS
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Source E.coli
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Code CSB-EP644152HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP644152HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP644152HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RSPO4
Uniprot No.
Alternative Names
C20orf182; CRISTIN4; hRspo4; R-spondin family, member 4; R-spondin-4; Roof plate-specific spondin-4; RSPO4; RSPO4_HUMAN
Species
Homo sapiens (Human)
Expression Region
20-234
Target Protein Sequence
L NRRKKQVGTG LGGNCTGCII CSEENGCSTC QQRLFLFIRR EGIRQYGKCL HDCPPGYFGI RGQEVNRCKK CGATCESCFS QDFCIRCKRQ FYLYKGKCLP TCPPGTLAHQ NTRECQGECE LGPWGGWSPC THNGKTCGSA WGLESRVREA GRAGHEEAAT CQVLSESRKC PIQRPCPGER SPGQKKGRKD RRPRKDRKLD RRLDVRPRQP GLQP
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway.
Gene References into Functions
  1. A novel nonsense mutation in RSPO4 gene involves in the development of anonychia congenita. PMID: 22300369
  2. c.3G>A mutation is not sufficient to cause the congenital hyponychia and could be considered a polymorphism. PMID: 23234511
  3. RSPO4 has a crucial role in nail morphogenesis. PMID: 17041604
  4. These findings expand our understanding of the role of RSPO4 in nail development and disease. PMID: 17805348
  5. The previously unknown mutations c.190C>T (p.Arg64Cys) in exon 2 and c.301C>T (p.Gln101X) in exon 3 were identified in RSPO4, thereby corroborating R-spondin 4 as the major protein in autosomal-recessive anonychia. PMID: 17914448
  6. identification of a missense mutation c.199G > C (p.Gly67Arg) in the RSPO4 gene in a large consanguineous Pakistani family with an autosomal recessive form of anonychia PMID: 18070203

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Involvement in disease
Nail disorder, non-syndromic congenital, 4 (NDNC4)
Subcellular Location
Secreted.
Protein Families
R-spondin family
Database Links

HGNC: 16175

OMIM: 206800

KEGG: hsa:343637

STRING: 9606.ENSP00000217260

UniGene: Hs.444980

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