Recombinant Human RBPJ-interacting and tubulin-associated protein (RITA)

Code CSB-YP842696HU
MSDS
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Source Yeast
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Code CSB-EP842696HU
MSDS
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Source E.coli
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Code CSB-EP842696HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP842696HU
MSDS
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Source Baculovirus
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Code CSB-MP842696HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RITA1
Uniprot No.
Alternative Names
RITA1; C12orf52; RITA; PSEC0043; RBPJ-interacting and tubulin-associated protein 1; RBPJ-interacting and tubulin-associated protein
Species
Homo sapiens (Human)
Expression Region
1-269
Target Protein Sequence
MKTPVELAVS GMQTLGLQHR CRGGYRVKAR TSYVDETLFG SPAGTRPTPP DFDPPWVEKA NRTRGVGKEA SKALGAKGSC ETTPSRGSTP TLTPRKKNKY RPISHTPSYC DESLFGSRSE GASFGAPRMA KGDAAKLRAL LWTPPPTPRG SHSPRPREAP LRAIHPAGPS KTEPGPAADS QKLSMGGLHS SRPLKRGLSH SLTHLNVPST GHPATSAPHT NGPQDLRPST SGVTFRSPLV TSRARSVSIS VPSTPRRGGA TQKPKPPWK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Tubulin-binding protein that acts as a negative regulator of Notch signaling pathway. Shuttles between the cytoplasm and the nucleus and mediates the nuclear export of RBPJ/RBPSUH, thereby preventing the interaction between RBPJ/RBPSUH and NICD product of Notch proteins (Notch intracellular domain), leading to down-regulate Notch-mediated transcription. May play a role in neurogenesis.
Gene References into Functions
  1. structural and biophysical studies demonstrate that RITA binds RBP-J similarly to the RAM (RBP-J-associated molecule) domain of Notch, our biochemical and cellular assays suggest that RITA interacts with additional regions in RBP-J. PMID: 28487372
  2. human RITA, binding to Drosophila Su(H) and tubulin, cannot influence the Notch signalling pathway in the fly PMID: 25588307
  3. RITA might suppress tumor growth and induce apoptosis in hepatocellular carcinoma. PMID: 24308154
Subcellular Location
Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Shuttles rapidly between the cytoplasm and the nucleus. The function of centrosome localization is still unclear.
Protein Families
RITA family
Database Links

HGNC: 25925

KEGG: hsa:84934

STRING: 9606.ENSP00000410395

UniGene: Hs.524762

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