Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

RBPMS

Uniprot No.

Q93062

Research Area

Epigenetics and Nuclear Signaling

Alternative Names

FLJ32971; Heart and RRM expressed sequence; Hermes; RBP MS; RBP-MS; Rbpms; RBPMS_HUMAN; RNA binding protein gene with multiple splicing; RNA-binding protein with multiple splicing

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-196aa

Target Protein Sequence

MNNGGKAEKENTPSEANLQEEEVRTLFVSGLPLDIKPRELYLLFRPFKGYEGSLIKLTSKQPVGFVSFDSRSEAEAAKNALNGIRFDPEIPQTLRLEFAKANTKMAKNKLVGTPNPSTPLPNTVPQFIAREPYELTVPALYPSSPEVWAPYPLYPAELAPALPPPAFTYPASLHAQMRWLPPSEATSQGWKSRQFC
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

28.8 kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Amino acids 1-196 form the expressed segment for recombinant Human RBPMS. The expected molecular weight for the RBPMS protein is calculated to be 28.8 kDa. This RBPMS recombinant protein is manufactured in e.coli. Fusion of the N-terminal 10xHis tag and C-terminal Myc tag into the RBPMS encoding gene fragment was conducted, allowing for easier detection and purification of the RBPMS protein in subsequent stages.

The main function of the human RNA-binding protein with multiple splicing (RBPMS) lies in its role as a regulator of RNA processing and splicing. RBPMS is pivotal in modulating alternative splicing events and influencing gene expression and cellular functions. In neuroscience, RBPMS is implicated in neuronal development and function, acting as a marker for specific neuronal populations. Furthermore, RBPMS is associated with cancer progression, indicating its potential as a prognostic marker and therapeutic target in oncology research. Investigating RBPMS offers insights into RNA biology, neurodevelopment, and cancer biology, providing avenues for understanding disease mechanisms and developing therapeutic strategies in diverse research areas.

Target Background

Function

Acts as a coactivator of transcriptional activity. Required to increase TGFB1/Smad-mediated transactivation. Acts through SMAD2, SMAD3 and SMAD4 to increase transcriptional activity. Increases phosphorylation of SMAD2 and SMAD3 on their C-terminal SSXS motif, possibly through recruitment of TGFBR1. Promotes the nuclear accumulation of SMAD2, SMAD3 and SMAD4 proteins. Binds to poly(A) RNA.

Gene References into Functions

The possible involvement of the GC box 1 at position - 54 in transcriptional regulation of Rbpms was corroborated by EMSA, which showed formation of a DNA-protein complex in the presence of the oligonucleotide corresponding to this Sp1-binding site. PMID: 29423656
Data indicate RNA Binding Protein with Multiple Splicing (RBPMS), Regulator of Chromosome Condensation and POZ Domain Containing Protein 1 (RCBTB1), and Zinc Finger protein 608 (ZNF608) as miR-21-3p target genes. PMID: 27166999
Conserved binding of GCAC motifs by MEC-8, couch potato, and the RBPMS protein family has been reported. PMID: 28003515
ERG is recruited to mRNAs via interaction with the RNA-binding protein RBPMS, and it promotes mRNA decay by binding CNOT2, a component of the CCR4-NOT deadenylation complex. PMID: 27273514
observation of a strong in vivo erythropoietic effect for RBPMS but not for GTF2E2, supporting the statistical fine-mapping at this locus and demonstrating that RBPMS is a regulator of erythropoiesis PMID: 28017375
Study indicates that the RNA binding increases the stability of RNA-recognition motif (RRM) in RBPMS domain, but residue mutations of RRM domain induce the fluctuation of complex systems through weakening of hydrogen bonds, conformational change or loss of binding affinity. PMID: 27592836
RBPMS1 is a critical repressor of AP-1 signaling and RBPMS1 activation may be a useful strategy for cancer treatment. PMID: 25281386

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Subcellular Location

Nucleus. Cytoplasm. Cytoplasm, P-body.

Tissue Specificity

Ubiquitously expressed, at various levels depending on the isoform and the tissue.

Database Links

HGNC: 19097

OMIM: 601558

KEGG: hsa:11030

STRING: 9606.ENSP00000340176

UniGene: Hs.334587

Code	CSB-EP821892HU
Abbreviation	Recombinant Human RBPMS protein
MSDS	MSDS Datasheet
Size	US$306
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Image	Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP821892HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) RBPMS. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP821892HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) RBPMS. (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human RNA-binding protein with multiple splicing (RBPMS)

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