Recombinant Human RNA polymerase II subunit A C-terminal domain phosphatase (CTDP1), partial

Code CSB-YP897499HU
MSDS
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Source Yeast
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Code CSB-EP897499HU
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Source E.coli
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Code CSB-EP897499HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP897499HU
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Source Baculovirus
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Code CSB-MP897499HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CTDP1
Uniprot No.
Alternative Names
CCFDN; CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) phosphatase, subunit 1; CTD of POLR2A, phosphatase of, subunit 1; CTDP1; CTDP1_HUMAN; EC 3.1.3.16 ; RNA polymerase II subunit A C terminal domain phosphatase; RNA polymerase II subunit A C-terminal domain phosphatase; Serine phosphatase FCP1a; TFIIF associating CTD phosphatase ; TFIIF-associating CTD phosphatase 1 ; TFIIF-associating CTD phosphatase; Transcription factor IIF-associating CTD phosphatase 1
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Processively dephosphorylates 'Ser-2' and 'Ser-5' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit. This promotes the activity of RNA polymerase II. Plays a role in the exit from mitosis by dephosphorylating crucial mitotic substrates (USP44, CDC20 and WEE1) that are required for M-phase-promoting factor (MPF)/CDK1 inactivation.
Gene References into Functions
  1. Study identifies CTDP1 as an autoantigen specific for Behcet Disease. PMID: 27777341
  2. results demonstrated that CTDP1 was upregulated in human lung cancer tissues. In addition, it implied that CTDP1 played an important role in cell proliferation and may be a useful therapeutic target in human lung cancer. PMID: 26590573
  3. Data propose that Fcp1 has a crucial role in the liaison between dephosphorylation and ubiquitination that drives mitosis exit. PMID: 22692537
  4. although FCP1 is intrinsically disordered, the above 16 residues composing the RAP74 binding surface form nascent alpha-helical structure in the unbound state. PMID: 21672523
  5. NMR assignments for C terminal region PMID: 19888685
  6. FCP1 augments tat transactivation of the HIV-1 long-terminal repeat PMID: 12036313
  7. FCP1 is important for transcription elongation along with TFIIF PMID: 12370301
  8. FCP1 binds to a groove of RAP74 between alpha-helices H2 and H3, without affecting the secondary structure of RAP74 C-terminal domain; FCP1 binds also to a groove of TFIIB core domain beween alpha helices D1 and E1 in the first cyclin repeat. PMID: 12578358
  9. FCP1 kinase from HeLa cells is phosphorylated and affects transcription elongation PMID: 12591939
  10. cocrystal structure of the winged-helix domain of human RNA polymerase II-associating protein 74 bound to the alpha-helical C terminus of human FCP1 PMID: 12591941
  11. high-resolution solution structure of a cterRAP74cterFCP complex by NMR spectroscopy PMID: 12732728
  12. Congenital cataracts facial dysmorphism neuropathy is caused by a single-nucleotide substitution in an antisense Alu element in intron 6 of CTDP1, resulting in a rare mechanism of aberrant splicing and an Alu insertion in the processed mRNA PMID: 14517542
  13. found that FCP1 is a genuine substrate of PRMT5-methylation both in vivo and in vitro, and FCP1-associated PRMT5 can methylate histones H4 in vitro PMID: 15670829
  14. Enhanced binding to RAP74 following phosphorylation. PMID: 15723517
  15. These data suggest that icariin exerts its potent osteogenic effect through induction of Runx2 expression, production of BMP-4 and activation of BMP signaling. PMID: 18294453
  16. NMR and thermodynamic studies further elucidate the complex molecular mechanism by which TFIIF and FCP1 cooperate for RNAPII recycling. PMID: 19215094

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Involvement in disease
Congenital cataracts, facial dysmorphism, and neuropathy (CCFDN)
Subcellular Location
Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Midbody. Note=Found at centrosomes in prometaphase, at spindle and spindle poles in metaphase and at spindle midzone and midbody in anaphase and telophase-G1 respectively.
Tissue Specificity
Ubiquitously expressed.
Database Links

HGNC: 2498

OMIM: 604168

KEGG: hsa:9150

STRING: 9606.ENSP00000299543

UniGene: Hs.465490

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