Recombinant Human Ral guanine nucleotide dissociation stimulator (RALGDS), partial

Code CSB-YP019299HU
MSDS
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Source Yeast
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Code CSB-EP019299HU
MSDS
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Source E.coli
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Code CSB-EP019299HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP019299HU
MSDS
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Source Baculovirus
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Code CSB-MP019299HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RALGDS
Uniprot No.
Alternative Names
RALGDS; KIAA1308; RGFRal guanine nucleotide dissociation stimulator; RalGDS; Ral guanine nucleotide exchange factor; RalGEF
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Stimulates the dissociation of GDP from the Ras-related RalA and RalB GTPases which allows GTP binding and activation of the GTPases. Interacts and acts as an effector molecule for R-Ras, H-Ras, K-Ras, and Rap.
Gene References into Functions
  1. Investigated is the role that electrostatic field differences caused by the charge reversal mutation Rap1A K31E play in determining the binding specificity of RalGDS to Rap1A versus Ras. PMID: 22738401
  2. involved in regulation of choline kinase activity by Ras proteins PMID: 11840339
  3. identified Ral-GDS as a beta-arrestin-binding protein by yeast two-hybrid screening and co-immunoprecipitation from human polymorphonuclear neutrophilic leukocytes PMID: 12105416
  4. RALGDS has a role in attenuating p53-independent DNA damage G2 checkpoint PMID: 15208305
  5. Merlin can function as a tumor suppressor by inhibiting the RalGDS-mediated oncogenic signals. PMID: 16007223
  6. results of screening suggest that RALGDS is not mutationally altered in cutaneous melanoma PMID: 17992125
  7. overexpression of RalGDS promotes exocytosis of Weibel-Palade bodies from endothelial cells PMID: 18417737
  8. RalGDS and RalA act downstream of Rheb and that RalA activation is a crucial step in nutrient-induced mTORC1 activation PMID: 18948269

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Subcellular Location
Cytoplasm. Nucleus.
Database Links

HGNC: 9842

OMIM: 601619

KEGG: hsa:5900

STRING: 9606.ENSP00000361120

UniGene: Hs.106185

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