Recombinant Human RalA-binding protein 1 (RALBP1)

Code CSB-YP618981HU
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Source Yeast
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Code CSB-EP618981HU
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Source E.coli
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Code CSB-EP618981HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP618981HU
MSDS
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Source Baculovirus
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Code CSB-MP618981HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RALBP1
Uniprot No.
Alternative Names
RLIP1; 76 kDa Ral-interacting protein; 76-kDa Ral-interacting protein; Dinitrophenyl S-glutathione ATPase; DNP-SG ATPase; Ral-interacting protein 1; Ral-interacting protein 1, 76-KD; RalA-binding protein 1; RalBP1; RBP1_HUMAN; RIP1; RLIP1; RLIP76
Species
Homo sapiens (Human)
Expression Region
2-655
Target Protein Sequence
TECFLPPTS SPSEHRRVEH GSGLTRTPSS EEISPTKFPG LYRTGEPSPP HDILHEPPDV VSDDEKDHGK KKGKFKKKEK RTEGYAAFQE DSSGDEAESP SKMKRSKGIH VFKKPSFSKK KEKDFKIKEK PKEEKHKEEK HKEEKHKEKK SKDLTAADVV KQWKEKKKKK KPIQEPEVPQ IDVPNLKPIF GIPLADAVER TMMYDGIRLP AVFRECIDYV EKYGMKCEGI YRVSGIKSKV DELKAAYDRE ESTNLEDYEP NTVASLLKQY LRDLPENLLT KELMPRFEEA CGRTTETEKV QEFQRLLKEL PECNYLLISW LIVHMDHVIA KELETKMNIQ NISIVLSPTV QISNRVLYVF FTHVQELFGN VVLKQVMKPL RWSNMATMPT LPETQAGIKE EIRRQEFLLN CLHRDLQGGI KDLSKEERLW EVQRILTALK RKLREAKRQE CETKIAQEIA SLSKEDVSKE EMNENEEVIN ILLAQENEIL TEQEELLAME QFLRRQIASE KEEIERLRAE IAEIQSRQQH GRSETEEYSS ESESESEDEE ELQIILEDLQ RQNEELEIKN NHLNQAIHEE REAIIELRVQ LRLLQMQRAK AEQQAQEDEE PEWRGGAVQP PRDGVLEPKA AKEQPKAGKE PAKPSPSRDR KETSI
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Multifunctional protein that functions as a downstream effector of RALA and RALB. As a GTPase-activating protein/GAP can inactivate CDC42 and RAC1 by stimulating their GTPase activity. As part of the Ral signaling pathway, may also regulate ligand-dependent EGF and insulin receptors-mediated endocytosis. During mitosis, may act as a scaffold protein in the phosphorylation of EPSIN/EPN1 by the mitotic kinase cyclin B-CDK1, preventing endocytosis during that phase of the cell cycle. During mitosis, also controls mitochondrial fission as an effector of RALA. Recruited to mitochondrion by RALA, acts as a scaffold to foster the mitotic kinase cyclin B-CDK1-mediated phosphorylation and activation of DNM1L.; Could also function as a primary ATP-dependent active transporter for glutathione conjugates of electrophiles. May also actively catalyze the efflux of a wide range of substrates including xenobiotics like doxorubicin (DOX) contributing to cell multidrug resistance.
Gene References into Functions
  1. RLIP76 plays a critical role in cellular proliferation, apoptosis, cell cycle distribution, cell movement and invasion in melanoma PMID: 28537681
  2. In the exons and exon-intron boundaries of ABCB5 and RLIP76 genes. PMID: 26975227
  3. the miR143-3p level was markedly lower in participants with ovarian cancer compared with normal control, while the expression of RALBP1 mRNA and protein were evidently overexpressed in participants with ovarian cancer compared with normal control. PMID: 27748916
  4. RLIP76 knockdown increased autophagic flux and apoptosis in U251 glioma cells. PMID: 27473470
  5. Phosphorylation level of Akt declined from 138.45+/-13.8 to 69.9+/-29.7% in SGC-7901, and from 115.5+/-26.6 to 49.07+/-27% in MGC-803 and phosphorylation level of mTOR also significantly decreased.While apoptosis of gastric cancer(GA) cells increased which we verified with apoptosis proteins and staining analysis. Our data showed that RLIP76 plays a significant oncogenic role in GC and it maybe a potential target in GC PMID: 27572296
  6. Anti-tumor effect was exerted when miR-124 directly targeted RLIP76, a stress-inducible transporter that plays a crucial role in the development of melanoma. PMID: 27657824
  7. report showed that RLIP76 expression was significantly increased in breast cancer samples and positively correlated with the malignant status of breast cancer patients; results indicated that high RLIP76 expression was associated with poor prognosis of breast cancer patients PMID: 26125275
  8. RLIP76 expression is induced by TNF-alpha and follows the induction kinetics of inflammation markers, suggesting that inflammation can influence RLIP76 expression at the blood brain barrier. PMID: 26406496
  9. RLIP76 downregulation in HT29 CRC cells suppressed cell growth, enhanced cell apoptosis, induced cell cycle arrest, and inhibited cell invasion by decreasing MMP2 expression. PMID: 25213293
  10. High RLIP76 expression is associated with a poor outcome of meningioma. PMID: 25993541
  11. results revealed that the effect of miR-101 on prostate cancer cell apoptosis was due to RLIP76 regulation of the PI3K/Akt/Bcl-2 signaling pathway PMID: 26067553
  12. RalBP1 protein is an independent predictor of poor survival and early relapse for CRC patients PMID: 22549157
  13. RLIP76 is a potential target for developing novel therapeutic strategies for leukemia PMID: 24839008
  14. RLIP76 is a node for Rho and Ras family signalling. [Review] PMID: 24450627
  15. Activation of RalBP1 during neoplastic epithelial cell transformation induces cytoplasmic accumulation of p27, this event requires p27 Ser-10 phosphorylation by protein kinase B/Akt. PMID: 23576547
  16. RLIP76 may suppress apoptosis and promote the proliferation of glioma cells by direct adenosine triphosphate-dependent xenobiotic transport and by activating the Rac1-JNK signaling pathway. PMID: 23276796
  17. p300 associates with the RLIP76 promoter via an overlapping cMYB and cETS binding site and regulates RLIP76 promoter activity and its expression. PMID: 23419874
  18. RalB-mediated invadopodium formation was dependent on RalBP1/RLIP76; disruption of the ATPase function of RalBP1 impaired invadopodium formation. PMID: 22331470
  19. The impairment of RLIP76 by aaRLIP76 can play a role in the damage of vascular cells from females, contributing to the gender-associated pathogenesis of immune-mediated vascular diseases. PMID: 21671802
  20. Data show that disrupting either RALA or RALBP1 leads to a loss of mitochondrial fission at mitosis, improper segregation of mitochondria during cytokinesis and a decrease in ATP levels and cell number. PMID: 21822277
  21. studies suggest that the expression of RalBP1 is necessary for human cancer cell metastasis; show that the requirement for RalA expression for manifestation of this phenotype is not entirely dependent on a RalA-RalBP1 interaction PMID: 21170262
  22. a link between RLIP76 mediated GS-E transport and cell cycle signaling are presented. PMID: 20183533
  23. Studies offer strong support for the hypothesis that RLIP76 is an overarching anti-apoptosis mechanism that, if inhibited, can be more broadly effective in the treatment of renal cell carcinoma. PMID: 19626587
  24. We have compared the transport properties of recombinant RLIP76 and human erythrocyte membrane RLIP76. PMID: 11732624
  25. In this review, RLIP76-mediated transport of organic ions has physiological and toxicological relevance which may play an important role in the mechanism of drug resistance. PMID: 12433796
  26. RLIP76 activity is a general determinant of 4HNE and DOX resistance. Its activity contributes to the drug-resistant phenotype of NSCLC. PMID: 12527936
  27. RLIP76 has a role in Doxorubicin transport in lung cancer PMID: 12632060
  28. RLIP76 has a role in triggering apoptosis in lung cancer cells and synergistically increaseing doxorubicin cytotoxicity PMID: 12632061
  29. RLIP/RalBP1 is used as a platform by the mitotic cdk1 to facilitate the phosphorylation of Epsin, which makes Epsin incompetent for endocytosis during mitosis, when endocytosis is switched off. PMID: 12775724
  30. These results show for the first time that POB1 can regulate the transport function of RLIP76 and are consistent with our previous studies showing that inhibition of RLIP76 induces apoptosis in cancer cells. PMID: 15707977
  31. Results identify targets in RLIP76 for phosphorylation by protein kinase C alpha, which may act as substrates for differential transport of doxorubicin. PMID: 16087181
  32. RLIP76 is the predominant transporter of antiepileptic drugs in the blood brain barrier and may be involved in mechanisms of drug-resistant epilepsy. PMID: 16188027
  33. Augmenting cellular levels of RLIP76 using purified recombinant RLIP76 increased growth rate in all cells, and restored the sensitivity of RLIP76-/- mouse embryonic fibroblasts to both inhibition through PKCalpha-depletion and stimulation through PMA. PMID: 16890208
  34. identify a role for caspase-8 in monocytes undergoing macrophagic differentiation, that is, the enzyme activated in an atypical complex down-regulates NF-kappaB activity through RIP1 cleavage. PMID: 17047155
  35. phosphoprotein mapping of Ral binding protein 1 (RalBP1/Rip1/RLIP76) PMID: 17706599
  36. the spatiotemporal mobilization of TICAM-1 in response to dsRNA and the formation of the TICAM-1 speckles containing RIP1 and NAP1 are important for the activation of the TLR3-TICAM-1 pathway. PMID: 17982077
  37. autoantibodies to RLIP76 play a pathogenetic role in immune-mediated vascular diseases PMID: 17993611
  38. common variants in RLIP76 are unlikely to contribute to epilepsy drug response. PMID: 18086001
  39. Hsf-1 causes specific and saturable inhibition of the transport activity of Ralbp1 and that the combination of Hsf-1 and POB1 causes nearly complete inhibition through specific bindings with Ralbp1. PMID: 18474607
  40. RLIP76 is a fundamental link between biochemical pathways and glutathione-linked metabolism of xenobiotics and stress-degense signaling pathways. PMID: 18628450
  41. RLIP76 serves a key effector function for survival of prostate cancer cells; depletion of RLIP76 in mice bearing xenografts of prostate cancer cells leads to near complete regression of established subcutaneous xenografts with no apparent toxic effects. PMID: 19073149
  42. the accumulation-deficient drug-resistance mediated by RLIP76 can be modulated by inhibition of RLIP76 transport activity by cdc2. PMID: 19375851
  43. RLIP76 is an anticancer for kidney cancer: inhibition of RLIP76 function by antibody or its depletion by small interfering RNA or antisense DNA causes marked regression of kidney xenografts in nude mice. PMID: 19417134

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Subcellular Location
Cell membrane; Peripheral membrane protein. Cytoplasm, cytosol. Cytoplasm, cytoskeleton, spindle pole. Nucleus. Mitochondrion.
Tissue Specificity
Expressed ubiquitously but at low levels. Shows a strong expression in the erythrocytes.
Database Links

HGNC: 9841

OMIM: 605801

KEGG: hsa:10928

STRING: 9606.ENSP00000019317

UniGene: Hs.528993

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