Recombinant Human Ras-related protein Rab-33B (RAB33B)

Code CSB-YP863938HU
MSDS
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Source Yeast
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Code CSB-EP863938HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP863938HU
MSDS
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Source Baculovirus
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Code CSB-MP863938HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RAB33B
Uniprot No.
Alternative Names
DKFZP434G099; MGC138182; RAB 33B; RAB33B; RAB33B member RAS oncogene family; Ras related protein Rab 33B; Ras related protein Rab33B; Ras-associated protein RAB33B; Ras-related protein Rab-33B; RB33B_HUMAN; SMC2
Species
Homo sapiens (Human)
Expression Region
1-229
Target Protein Sequence
MAEEMESSLE ASFSSSGAVS GASGFLPPAR SRIFKIIVIG DSNVGKTCLT YRFCAGRFPD RTEATIGVDF RERAVEIDGE RIKIQLWDTA GQERFRKSMV QHYYRNVHAV VFVYDMTNMA SFHSLPSWIE ECKQHLLAND IPRILVGNKC DLRSAIQVPT DLAQKFADTH SMPLFETSAK NPNDNDHVEA IFMTLAHKLK SHKPLMLSQP PDNGIILKPE PKPAMTCWC
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Protein transport. Acts, in coordination with RAB6A, to regulate intra-Golgi retrograde trafficking. It is involved in autophagy, acting as a modulator of autophagosome formation.
Gene References into Functions
  1. Rab33b, OATL1 and Myo6 have roles in nanoparticle trafficking in HeLa cells PMID: 27374232
  2. Together, these results indicate that Rab33B is an important player in intracellular hepatitis B virus trafficking events, guiding core transport to nucleocapsid assembly sites and/or nucleocapsid transport to budding sites. PMID: 28635671
  3. Here we present three SMC patients with four novel pathogenic variants in RAB33B, including homozygosity for c.211C>T (p.R71*), homozygosity for c.365T>C (p.F122S), and compound heterozygosity for c.48delCGGGGCAG (p.G17Vfs*58) and c.490C>T (p.Q164*). PMID: 28127940
  4. Arg-24 of Atg16L1 is crucial for its interaction with Atg5 which has implications in the binding of the dimeric complex to Rab33B PMID: 26975471
  5. Analysis of hepatitis B virus capsid maturation steps revealed that Rab33B and Atg5/12/16L1 are required for proper particle assembly and/or stability. PMID: 25439980
  6. Ric1-Rgp1 complex is a guanine nucleotide exchange factor for the late Golgi Rab6A GTPase and an effector of the medial Golgi Rab33B GTPase. PMID: 23091056
  7. A second autosomal recessive locus for Dyggve-Melchior-Clausen syndrome exists on 4q31.1 and that Rab protein-encoding gene, RAB33B, is the likely disease gene in that locus. PMID: 22652534

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Involvement in disease
Smith-McCort dysplasia 2 (SMC2)
Subcellular Location
Golgi apparatus membrane; Lipid-anchor. Golgi apparatus, cis-Golgi network.
Protein Families
Small GTPase superfamily, Rab family
Database Links

HGNC: 16075

OMIM: 605950

KEGG: hsa:83452

STRING: 9606.ENSP00000306496

UniGene: Hs.591679

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