Recombinant Human Reelin(RELN),partial

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Code CSB-EP019557HU
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names RELN
Uniprot No. P78509
Research Area Cell Adhesion
Alternative Names LIS2; PRO1598; Reeler; Reelin; RELN; RELN_HUMAN; RL
Species Homo sapiens (Human)
Source E.coli
Expression Region 26-254aa
Target Protein Sequence AAGYYPRFSPFFFLCTHHGELEGDGEQGEVLISLHIAGNPTYYVPGQEYHVTISTSTFFDGLLVTGLYTSTSVQASQSIGGSSAFGFGIMSDHQFGNQFMCSVVASHVSHLPTTNLSFIWIAPPAGTGCVNFMATATHRGQVIFKDALAQQLCEQGAPTDVTVHPHLAEIHSDSIILRDDFDSYHQLQLNPNIWVECNNCETGEQCGAIMHGNAVTFCEPYGPRELITT
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 28.9kDa
Protein Length Partial
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Extracellular matrix serine protease that plays a role in layering of neurons in the cerebral cortex and cerebellum. Regulates microtubule function in neurons and neuronal migration. Affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion. Binding to the extracellular domains of lipoprotein receptors VLDLR and LRP8/APOER2 induces tyrosine phosphorylation of DAB1 and modulation of TAU phosphorylation (By similarity).
Gene References into Functions
  1. Study found a positive link between RELN SNP rs2229864 and ASD risk, but not a significant association between RELN SNPs rs736707 and autism risk. A significant rs736707-rs2229864 haplotype was found. Individuals with a CC haplotype were more likely to have autistic spectrum disorders, whereas individuals with a CT haplotype had more possibility to be typical development controls. PMID: 29753726
  2. A two-hit loss involving VHL predicted for clear-cell renal cell carcinoma(ccRCC ) and a better prognosis, whereas mutations in PTEN, TP53, or RELN predicted for Sarcomatoid clear-cell renal cell carcinoma and worse prognosis. PMID: 28710314
  3. we determined that Reelin protein and mRNA levels increased in CJD human samples and in mouse models of human prion disease in contrast to murine models of prion infection. However, changes in Reelin expression appeared only at late terminal stages of the disease, which prevent their use as an efficient diagnostic biomarker. PMID: 27726110
  4. In primary-culture fetal astrocytes, streptozotocin down-regulated the expression of Reelin 410 and 180 kDa , while alpha-boswellic acid upregulated it. Interruption in astroglial Reelin/Akt/Tau signaling pathways may have a role in Alzheimer disease. PMID: 27567921
  5. In frontal cortex extracts, an increase in Reelin mRNA, and in soluble and insoluble (guanidine-extractable) Reelin protein, was associated with late Braak stages of Alzheimer's disease (AD), while expression of its receptor, ApoER2, did not change. PMID: 27531658
  6. This study detected significant reductions in the mRNAs associated with RELN and GAD1 in the frontal cortex (FC) of autism spectrum disorder. PMID: 28229923
  7. There results suggest that reelin played essential roles in the development of lymphoma and might be a potential drug target in lymphoma PMID: 28498462
  8. This review addresses recent advances in the field of nonneuronal reelin signaling. PMID: 27739126
  9. methylation status of the promoter proximal cytosine-phospho-guanine dinucleotides determines the expression of RELN in myeloma cells. PMID: 27245998
  10. Meta-analyses of 12 RELN gene single nucleotide polymorphisms (SNPs) and related neuropsychiatric disorders (schizophrenia, autistic spectrum disorders, attention-deficit hyperactivity disorder, Alzheimer's disease and bipolar disorders) with subgroup analyses based on ethnicity. Findings suggest a role of RELN SNPs in psychiatric diseases. PMID: 28506622
  11. review of role in Alzheimer's disease PMID: 27031488
  12. Significant association between rs17458357 , rs2572683,rs12555895 within the RELN gene and accelerated decline in Cognition performance in Chinese elderly male Gout population. PMID: 28953682
  13. This study demonstrated that RELN DNA methylation might contribute to the pathogenesis of schizophrenia. PMID: 28086126
  14. The present investigation, performed on a study sample from a population with one of the highest suicide rates in the world, indicated an association between rs2965087 in the reelin gene and the expression of suicidal threats a month before suicide in contrast to other symptoms of depression. PMID: 27537376
  15. Reelin is was low in primary breast cancer tissue, but higher in Her2(+) breast cancers (but not triple-negative ones) metastasizing to the brain, especially in the tumor periphery adjacent to surrounding astrocytes. In the neural niche, astrocytes epigenetically regulate Reelin expression and its interaction with Her2 leading to increased proliferation and survival fitness. PMID: 28210910
  16. Heterozygous RELN mutations cause a typical Autosomal Dominant Lateral Temporal Lobe Epilepsy syndrome, indistinguishable from that associated with LGI1 mutations. PMID: 28142128
  17. We screened for mutations in RELN or VLDLR and compared the phenotype of these patients with that of previously reported patients. differences in clinical severity, involvement of the cerebellar hemispheres, together with the severity of the neocortical defect, enables RELN-mutated patients to be distinguished from VLDLR-mutated patients. PMID: 27000652
  18. Reelin signaling is a critical player in the modulation of synaptic function. PMID: 27994051
  19. Reelin was expressed by human hepatic stellate cells/myofibroblasts and the number of these cells increased significantly in the lobule as the liver fibrosis progressed, suggesting a role for Reelin in the activation of hepatic stellate cells/myofibroblasts during liver injury. PMID: 28348420
  20. The presence of reelin was elevated in junctional areas as in dysplastic nevi. VLDLR presented positive values in 16 cases (16/ 32) and ApoER2 was weak positive in 7 cases. PMID: 28255385
  21. common variants of GABRG2, RELN and NRG3 and the GABRG2-RELN-PTCH1 interaction networks might confer altered susceptibility to Hirschsprung disease. PMID: 27889765
  22. RELN is mapped at 7q22, which had been identified as a candidate region for autism by early genetic linkage study. PMID: 26285919
  23. reelin promotes multiple myeloma cell adhesion, survival, and drug resistance via activation of integrin alpha5beta1. PMID: 26848618
  24. These findings suggest that circulating Reelin promotes atherosclerosis by increasing vascular inflammation, and that reducing or inhibiting circulating Reelin may present a novel approach for the prevention of cardiovascular disease. PMID: 26980442
  25. Reelin thus plays a role in restraining RAS and PI3-kinase promotion of cell motility and potentially tumour metastasis. PMID: 27071537
  26. Among men, but not in women certain genotypes of the RELN gene were significantly associated with the susceptibility to Alzheimer's disease. PMID: 26384575
  27. Report demonstrated that the reelin subregion R5-6 consisting of 747 amino acids in the 5th and 6th repeats was sufficient for apoER2 and VLDLR binding, and inhibiting lipoprotein-induced cholesterol accumulation in macrophages. PMID: 26317415
  28. The features of reelin expression in the brain of fetuses and newborns at 22-40 weeks' gestation with internal HC should be considered as morphological differential and diagnostic criteria for the disease in relation to its etiology. PMID: 26978229
  29. Study shows that early neural cells transiently express Reelin at the time they leave the presumptive olfactory/vomeronasal epithelium and that Dab 1 is present in the migratory cell mass and in the presumptive ensheathing cells in the absence of reelin. PMID: 26270645
  30. the reelin protein blood concentration might be a relevant signal with respect to the pathophysiology of schizophrenia. PMID: 26305216
  31. These findings suggest a central AKT-FOXG1-reelin signaling pathway in focal malformations of cortical development and support pathway inhibitors as potential treatments or therapies for some forms of focal epilepsy. PMID: 26523971
  32. rs7341475 (A/G) and rs262355 (A/T) polymorphisms in RELN gene are inversely associated with SZ risk. PMID: 26455866
  33. RELN SNPs were associated with Alzheimer disease and mild cognitive impairment. GG genotype at rs2299356 was associated with risk of AD. RELN-rs528528 CT genotype was protective for MCI. PMID: 24384746
  34. pThis study roposed that RELN mutations contribute to the genetic heterogeneity of myoclonus-dystonia. PMID: 25648840
  35. Heterozygous reelin mutations cause autosomal-dominant lateral temporal epilepsy. PMID: 26046367
  36. serum reelin may be considered an additional useful parameter for monitoring the progression of hepatic fibrosis in HCV-infected patients specially in those with active rheumatological conditions which result in an increase in serum hyaluronic acid PMID: 24803609
  37. RELN expression in the cerebral cortex of subjects with autism is not a consequence of decreased numbers of RELN-expressing neurons PMID: 25067827
  38. RELN gene polymorphism rs7341475 C>T is associated with the risk of paranoid schizophrenia in Russians and Tatars. PMID: 25842846
  39. The results of this study demonstrated the presence of reelin, its receptors VLDLR and ApoER2 as well as Dab1 in the ENS and might indicate a novel role of the reelin system in regulating neuronal plasticity and pre-synaptic functions in the ENS. PMID: 24844606
  40. Results indicate that aging- and disease-associated changes in Reelin levels and proteolytic processing might play a role in the formation of corpora amylacea by altering cytoskeletal dynamics PMID: 24252415
  41. The g.504742G>A polymorphic variant in the RELN gene might affect subjects susceptibility toward autism in Chinese Han population. PMID: 23287318
  42. Lack of association for RELN is that RELN could be associated with a specific otosclerosis-like phenotype that is different from the histologically confirmed phenotype of the patients in this study. PMID: 24643032
  43. In this population, the pathogenic link between the rs39335 variant and otosclerosis was excluded. PMID: 24227897
  44. Data indicate taht reelin interacts with amyloid beta-protein(1-42) and is sequestered by fibrils. PMID: 24599114
  45. the RELN rs362691, rather than rs736707 or GGC repeat variant, might contribute significantly to Autism spectrum disorder risk PMID: 24453138
  46. Data suggest that PS1/gamma-secretase-dependent processing of the reelin receptor ApoER2 inhibits reelin expression and may regulate its signaling. PMID: 24344333
  47. reelin expression is altered by Abeta leading to impaired reelin signaling. PMID: 23951306
  48. In this review, the signaling protein Reelin, expressed along olfactory and limbic pathways is involved in the early stages of Alzheimer's disease. PMID: 23632168
  49. Data indicate that Reelin is up-regulated during erythroid differentiation of erythroleukemic K562 cells. PMID: 24239537
  50. moderate risk of bias show a statistically significant association with the ACE gene, AGT gene, OTSC2, RELN gene, TGFB1 gene, 11q13.1, OTSC2, OTSC5, OTSC8, and OTSC10. [Review] PMID: 24170657
  51. results of haplotype analysis might suggest a positive association between variant in RELN and schizophrenia in Chinese Va people PMID: 23277132
  52. RELN is a susceptibility gene for schizophrenia in Chinese population. PMID: 21745129
  53. A subset of Focal Cortical Dysplasia type IIIa exists in which loss of Reelin appears to be the common pathogenetic basis. PMID: 22203331
  54. A significant association of intronic SNP rs736707, but not for exonci SNP rs362691, with autism in the South African population is observed. PMID: 23216241
  55. In human nodular heterotopia specimens, small reelin-positive cells were found in the nodules, corroborating the hypothesis that reelin might be involved in human heterotopic nodular formation. PMID: 23274163
  56. Decline of RELN expression in the development of Alzheimer's disease (AD) pathology supports RELN's potential as a pre-clinical marker for AD. PMID: 22495348
  57. The serine protease tissue plasminogen activator (tPA) and two matrix metalloproteinases, ADAMTS-4 and ADAMTS-5, were identified as Reelin cleaving enzymes. PMID: 23082219
  58. Polyglutamine expansion decreased ATXN7 occupancy, which correlated with increased levels of histone H2B monoubiquitination, at the reelin promoter. PMID: 23236151
  59. Since reelin is responsible for migration and synapse formation, the decreased gene expression of reelin in the left prefrontal area of schizophrenia patients points to neurodevelopmental deficits in neuronal migration and synaptic plasticity. PMID: 22797278
  60. The reelin pathway has a cell-autonomous and critical role in regulating dendritic development and the integration of adult-generated granule cells, via a pathway that is a key regulator of adult neurogenesis. PMID: 22933789
  61. Reelin has a dual role in neuroblastoma Autocrine expression marks low-grade differentiating tumour cells, whereas paracrine Reelin may act as a chemoattractant and promote hematogenic and lymphogenic dissemination in progressed stages. PMID: 22614235
  62. Variation in genes encoding proteins at the gateway of Reelin signaling: ligands RELN and APOE, their common receptors APOER2 and VLDLR, and adaptor DAB1, was examined. PMID: 22419519
  63. In mice with an overexpression of reelin protein, behavioral phenotypes related to schizophrenia and bipolar disorder are prevented. PMID: 21814183
  64. Reelin signaling pathways modulate phosphorylation of tau, the major component of neurofibrillary tangles, either directly or through beta-amyloid pathways that influence tau phosphorylation PMID: 20452100
  65. show that Reelin-stimulated Notch-1 activation is dependent on Reelin signaling PMID: 22394407
  66. Reelin is an essential negative regulator in the TGF-beta1-induced cell migration process, and is suppressed by TGF-beta pathway at the transcriptional level through Snail regulation PMID: 22393371
  67. As being a part of the otic capsule, stapes footplate might be characterized by persisting reelin detectability without mRNA expression. Between these conditions, the etiologic role of RELN is questionable in the pathogenesis of otosclerosis. PMID: 21630058
  68. Single nucleotide polymorphism of rs7341475 in intron 4 of RELN contributed to Schizophrenia susceptibility in the Chinese population. PMID: 22006801
  69. The RELN promoter did not harbor risk SNPs associated with schizophrenia in the Chinese population. PMID: 22006802
  70. rs362719 of the RELN gene is associated with susceptibility to schizophrenia in Chinese Han, possibly through a gender-specific mechanism. PMID: 21863557
  71. the exon 22 C/G marker of Reelin is significantly associated with Alzheimer 's disease in the Greek population PMID: 22023759
  72. analysis of the covalent homodimer of reelin protein linked via its central region PMID: 21844191
  73. This study describes a positive association between RELN and SZ in the Han Chinese population, and provides genetic evidence to support the gender difference of SZ. PMID: 21549172
  74. study further supports the connection between RELN dysfunction and psychiatric disorders, and provides a possible functional role for a schizophrenia associated SNP. PMID: 21603580
  75. The results of this study did not support previous positive findings and suggest that the four single-nucleotide polymorphisms (SNP) of RELN are unlikely to be associated with childhood autism in Chinese Han population. PMID: 20554015
  76. Reduced expression of reelin gene is associated with high recurrence rate of hepatocellular carcinoma. PMID: 20734148
  77. Association of exon 22 G/C marker or any marker in the vicinity, which is in linkage disequilibrium with this marker with epilepsy in the West Bengal population. PMID: 20697953
  78. The presence of ectopically expressed Reelin attracting migrating neurons might be a common mechanism. In patients with a genetic defect the nodular heterotopia are almost exclusively bilateral and located in the periventricular region. PMID: 21395570
  79. Transgenic reelin has a direct role in promoting rapid morphological differentation and orientation of prospective layer (L6) neurons during preplate splitting. PMID: 20064940
  80. In the absence of reelin, cortical layers fail to form due to inappropriate neuron migration and positioning in mouse chimeras. PMID: 20053715
  81. Reelin regulates cadherin function via Dab1/Rap1 signal pathway to control migrating neurons and lamination in the neocortex. PMID: 21315259
  82. Data suggest that reelin may play an important role in controlling invasiveness and metastatic potential of breast cancer cells and that its expression is controlled by promoter methylation. PMID: 20847288
  83. Among 12 SNPs in the RELN gene, the SNPs rs2711870 and rs2249372 showed significant associations with perseverative error processing. For both SNPs, the A-alleles were related to higher numbers of perseverative errors as compared to the GG genotype. PMID: 20727978
  84. the Reelin gene is associated with bipolar disorder in a sex-specific manner PMID: 19691043
  85. The association between rs7341475 and schizophrenia in women was significant (P = 9.0 x 10(-3)), with a calculated odds ratio (OR) of 1.11, and the association between rs17746501 and schizophrenia was significant in four of the new samples. PMID: 20468075
  86. Reelin levels are increased in synovial fluid of patients with rheumatoid arthritis (RA). It could play an important role in RA development and maintenance. PMID: 20659411
  87. Data show the lack of association between SNP rs3914132 of the RELN gene and otosclerosis in India. PMID: 20882487
  88. Association of single nucleotide polymorphism (rs7341475) in RELN with schizophrenia was replicated in women in another large independent Ashkenazi Jewish collection and confirmed that it applies to both SZ and schizoaffective disorder. PMID: 20431428
  89. This study does not suggest a significant impact of rs7341475 on brain structure, function, and RELN expression. PMID: 20434133
  90. Four SNPs were significantly associated with otosclerosis; three SNPs are located in the RELN region and the last one is located in the region on chromosome 11. PMID: 20642811
  91. cognitive functions were detected among the affected individuals. We thus propose a particular role for RELN as a modifier gene of the pathogenesis of schizophrenia. PMID: 19922905
  92. These results provide evidence that Reelin expression and processing is altered in several amyloid conditions. PMID: 20025970
  93. Differential functions of ApoER2 and very low density lipoprotein receptor in Reelin signaling depend on differential sorting of the receptors. PMID: 19948739
  94. finding suggests that lack of reelin impairs GABAergic Purkinje neuron expression and/or positioning during cerebellar development PMID: 20150511
  95. Reelin, one of the autism candidates, is necessary in regulation of neuronal migration during brain development and also in maintaining synaptic plasticity during postnatal life period PMID: 20010491
  96. in epilepsy reelin dysfunction causes granule cell dispersion development, and reelin is important for the maintenance of layered structures in the adult brain [review] PMID: 19633980
  97. disruption of the RELN pathway may be involved in gastric carcinogenesis PMID: 19956836
  98. Data further implicate RELN in the pathogenesis of otosclerosis. PMID: 19847460
  99. ApoEr2 regulates cell movement, and both X11alpha and Reelin enhance this effect. PMID: 19720620
  100. These findings demonstrate for the first time that reelin is present in platelets and the reelin-to-platelet interactions play a novel role in platelet signaling and functions. PMID: 19936619

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Involvement in disease Lissencephaly 2 (LIS2); Epilepsy, familial temporal lobe, 7 (ETL7)
Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families Reelin family
Tissue Specificity Abundantly produced during brain ontogenesis by the Cajal-Retzius cells and other pioneer neurons located in the telencephalic marginal zone and by granule cells of the external granular layer of the cerebellum. In adult brain, preferentially expressed in
Database Links

HGNC: 9957

OMIM: 257320

KEGG: hsa:5649

STRING: 9606.ENSP00000392423

UniGene: Hs.655654

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