This recombinant human renin receptor (ATP6AP2) comes from E.coli production, with an expression region covering amino acids 17-350—essentially the complete mature protein. It carries an N-terminal 6xHis-SUMO tag that aids in both purification and detection. SDS-PAGE analysis confirms the product reaches greater than 90% purity, which appears to support reliable research outcomes.
The renin receptor, or ATP6AP2, seems to play an important role in the renin-angiotensin system that helps control blood pressure and electrolyte balance. Beyond these pathways, ATP6AP2 is likely involved in cellular processes like proton transport and protein folding. This makes it particularly interesting for cardiovascular and metabolic research.
Potential Applications
Note: The applications listed below are based on what we know about this protein's biological functions, published research, and experience from experts in the field. However, we haven't fully tested all of these applications ourselves yet. We'd recommend running some preliminary tests first to make sure they work for your specific research goals.
1. Protein-Protein Interaction Studies Using Pull-Down Assays
The N-terminal 6xHis-SUMO tag makes nickel affinity-based pull-down experiments possible for identifying potential binding partners of the renin receptor. With >90% purity, there's minimal background interference from contaminating proteins during interaction studies. Researchers can attach this recombinant protein to nickel-coated beads and mix it with cell lysates or purified protein libraries to capture interacting molecules. The SUMO tag may provide extra structural stability and could help the protein fold properly for more natural interactions.
2. Antibody Development and Validation
This highly pure recombinant ATP6AP2 protein works well as an immunogen for creating specific antibodies against the human renin receptor. The 334-amino acid construct (17-350aa) covers a substantial portion of the mature protein, offering multiple epitopes for antibody recognition. The His-SUMO tag can be used in ELISA-based screening assays to find high-affinity antibodies during hybridoma selection. The protein also serves as a positive control when validating newly developed antibodies through Western blot analysis.
3. Biochemical Characterization and Stability Studies
This recombinant protein allows for thorough biochemical analysis—thermal stability profiling, pH tolerance testing, and buffer optimization studies. The >90% purity should enable accurate protein concentration determination and reliable biophysical measurements. Scientists can examine how the protein behaves under different conditions to establish the best storage and handling protocols. The His tag makes purification straightforward for repeated experiments and allows concentration adjustments for dose-response studies.
4. In Vitro Binding Assays with Known Ligands
This recombinant ATP6AP2 can be used to study binding interactions with known renin receptor ligands or potential small molecule compounds in plate-based assays. The His tag allows for straightforward attachment to nickel-coated surfaces for surface plasmon resonance or other label-free binding studies. High purity should reduce non-specific binding events that might interfere with binding affinity measurements. Such studies may provide valuable kinetic and thermodynamic parameters for receptor-ligand interactions.
