Recombinant Human Ret finger protein-like 1 (RFPL1)

Code CSB-YP019597HU
MSDS
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Source Yeast
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Code CSB-EP019597HU
MSDS
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Source E.coli
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Code CSB-EP019597HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP019597HU
MSDS
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Source Baculovirus
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Code CSB-MP019597HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RFPL1
Uniprot No.
Alternative Names
RFPL1; RFPL1L; RNF78; Ret finger protein-like 1; RING finger protein 78
Species
Homo sapiens (Human)
Expression Region
1-317
Target Protein Sequence
MKRLSLVTTN RLSPHGNFLP LCTFPLAVDM AALFQEASSC PVCSDYLEKP MSLECGCAVC FKCINSLQKE PHGEDLLCCC CSMVSQKNKI RPSWQLERLA SHIKELEPKL KKILQMNPRM RKFQVDMTLD ADTANNFLLI SDDLRSVRSG CITQNRQDLA ERFDVSICIL GSPRFTCGRH YWEVDVGTST EWDLGVCRES VHRKGRIHLT TERGFWTVSL RDGSRLSAST VPLTFLFVDR KLQRVGIFLD MGMQNVSFFD AEGGSHVYTF RSVSAEEPLH LFFAPPSPPN GDKSVLSICP VINPGTTDAP VHPGEAK
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Negatively regulates the G2-M phase transition, possibly by promoting cyclin B1/CCNB1 and CDK1 proteasomal degradation and thereby preventing their accumulation during interphase.
Gene References into Functions
  1. hRFPL1 antiproliferative activity led to an increased cell population in G(2)/M phase and specific cyclin B1 and Cdc2 downregulations. PMID: 20725088
  2. the human RFPL1,2,3 genes have a role in neocortex development PMID: 18656177
Subcellular Location
Cytoplasm. Nucleus.
Tissue Specificity
Seems to be expressed in prostate and less abundantly in adult brain, fetal liver, and fetal kidney.
Database Links

HGNC: 9977

OMIM: 605968

KEGG: hsa:5988

STRING: 9606.ENSP00000346342

UniGene: Hs.648249

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