Recombinant Human Rho GTPase-activating protein 11B (ARHGAP11B)

Code CSB-YP664668HU
MSDS
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Source Yeast
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Code CSB-EP664668HU
MSDS
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Source E.coli
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Code CSB-EP664668HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP664668HU
MSDS
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Source Baculovirus
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Code CSB-MP664668HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ARHGAP11B
Uniprot No.
Alternative Names
ARHGAP11B; FAM7B1; Rho GTPase-activating protein 11B; Protein FAM7B1; Rho-type GTPase-activating protein 11B
Species
Homo sapiens (Human)
Expression Region
1-267
Target Protein Sequence
MWDQRLVKLA LLQHLRAFYG IKVKGVRGQC DRRRHETAAT EIGGKIFGVP FNALPHSAVP EYGHIPSFLV DACTSLEEHI HTEGLFRKSG SVIRLKALKN KVDHGEGCLS SAPPCDIAGL LKQFFRELPE PILPADLHEA LLKAQQLGTE EKNKAILLLS CLLADHTVHV LRYFFNFLRN VSLRSSENKM DSSNLAVIFA PNLLQTSEGH EKMSSNAEKK GVYQTLSWKR YQPCWVLMVS VLLHHWKALK KVNMKLLVNI REREDNV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Hominin-specific protein that promotes development and evolutionary expansion of the brain neocortex. Able to promote amplification of basal progenitors in the subventricular zone, producing more neurons during fetal corticogenesis, thereby playing a key role in neocortex expansion. Promotes the proliferation of basal progenitors by inhibiting the mitochondrial permeability transition pore (mPTP): delays the opening of the mPTP via interaction with ADP:ATP translocase, thereby increasing mitochondrial Ca(2+) concentration and inducing glutamine catabolism, which is required for basal progenitor proliferation. Does not possess GTPase activator activity: the absence of GTPase activator activity is required to promote amplification of basal progenitors during neocortex development.
Gene References into Functions
  1. The data also demonstrate that a single C-->G base substitution underlies the ARHGAP11B-mediated basal progenitor amplification implicated in neocortex expansion. PMID: 27957544
  2. ARHGAP11B may have contributed to evolutionary expansion of human neocortex. PMID: 25721503
Subcellular Location
Mitochondrion matrix.
Database Links

HGNC: 15782

OMIM: 616310

KEGG: hsa:89839

STRING: 9606.ENSP00000392760

UniGene: Hs.659621

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