Recombinant Human Rho GTPase-activating protein 21 (ARHGAP21), partial

Product Details

Purity

≥85% (SDS-PAGE)

Target Names

ARHGAP21

Uniprot No.

Alternative Names

ARHGAP21; ARHGAP10; KIAA1424; Rho GTPase-activating protein 21; Rho GTPase-activating protein 10; Rho-type GTPase-activating protein 21

Species

Homo sapiens (Human)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Functions as a GTPase-activating protein (GAP) for RHOA and CDC42. Downstream partner of ARF1 which may control Golgi apparatus structure and function. Also required for CTNNA1 recruitment to adherens junctions.

Gene References into Functions

Reduced Arhgap21 is associated with Hematopoietic defects. PMID: 29212046
The results show that PTEN controls multicellular assembly through a membrane-associated regulatory protein complex composed of beta-Arrestin1, ARHGAP21 and Cdc42. PMID: 28749339
Gly1121Ser variant in ARHGAP21 gene found to be shared by all MP individuals in arger branch of family with nearly complete penetrance. ARHGAP21 protein strengthens cell-cell adhesions; may be regulated by BMPs influencing mandibular growth. PMID: 25691070
ARHGAP21 is a Rho-GAP involved in cell-cell junction remodeling that affects migration and EMT through alpha-tubulin interaction and acetylation PMID: 23235160
Co-immunoprecipitations and in vitro SUMOylation confirmed ARHGAP21 specific modification by SUMO2/3 and mapped the SUMOylation site to ARHGAP21 lysine K1443. PMID: 22922005
silencing Cdc42 reduced influenza A virus replication, whereas silencing ARHGAP21 increased the virus replication. PMID: 22318733
Disruption of the beta-arrestin 1/ARHGAP21 complex results in a more active ARHGAP21, leading to less efficient signaling via the angiotensin II type 1A receptor and, thereby, attenuation of stimulated stress fiber formation. PMID: 21173159
ARHGAP10 gene is highly expressed in muscle and brain, which are highly differentiated tissues. It supports the hypothesis that ARHGAP10 is important for cell differentiation PMID: 12056806
The ARHGAP10 protein is a novel Rho GTPase-activating protein (Rho-GAP) that is recruited to Golgi membranes through binding to GTP-ARF1. PMID: 15793564
The crystal structure of the activated GTP-bound form of ARF1 in a complex with the Arf-binding domain (ArfBD) of ARHGAP21 at 2.1 A resolution, is presented. PMID: 17347647
Data demonstrate that the RhoGTPase activating protein 21 (ARHGAP21) is expressed in the nuclear and perinuclear regions of several cell lines, and interacts with the C-terminal region of focal adhesion kinase. PMID: 19268501

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Subcellular Location

Golgi apparatus membrane; Peripheral membrane protein. Cell junction. Cytoplasmic vesicle membrane; Peripheral membrane protein. Cytoplasm, cytoskeleton. Note=Localization to the Golgi is dependent on interaction with GTP-bound ARF1.

Tissue Specificity

Widely expressed with higher expression in brain, heart, skeletal muscle and placenta.

Database Links

HGNC: 23725

OMIM: 609870

KEGG: hsa:57584

STRING: 9606.ENSP00000379709

UniGene: Hs.524195

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Recombinant Human Rho GTPase-activating protein 21 (ARHGAP21), partial

Product Details

Customer Reviews and Q&A

Target Background

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