Recombinant Human Rho GTPase-activating protein 36 (ARHGAP36)

Code CSB-YP744411HU
MSDS
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Source Yeast
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Code CSB-EP744411HU
MSDS
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Source E.coli
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Code CSB-EP744411HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP744411HU
MSDS
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Source Baculovirus
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Code CSB-MP744411HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ARHGAP36
Uniprot No.
Alternative Names
ARHGAP36; Rho GTPase-activating protein 36
Species
Homo sapiens (Human)
Expression Region
41-547
Target Protein Sequence
HNPDRRTKMV SIHSLSELER LKLQETAYHE LVARHFLSEF KPDRALPIDR PNTLDKWFLI LRGQQRAVSH KTFGISLEEV LVNEFTRRKH LELTATMQVE EATGQAAGRR RGNVVRRVFG RIRRFFSRRR NEPTLPREFT RRGRRGAVSV DSLAELEDGA LLLQTLQLSK ISFPIGQRLL GSKRKMSLNP IAKQIPQVVE ACCQFIEKHG LSAVGIFTLE YSVQRVRQLR EEFDQGLDVV LDDNQNVHDV AALLKEFFRD MKDSLLPDDL YMSFLLTATL KPQDQLSALQ LLVYLMPPCH SDTLERLLKA LHKITENCED SIGIDGQLVP GNRMTSTNLA LVFGSALLKK GKFGKRESRK TKLGIDHYVA SVNVVRAMID NWDVLFQVPP HIQRQVAKRV WKSSPEALDF IRRRNLRKIQ SARIKMEEDA LLSDPVETSA EARAAVLAQS KPSDEGSSEE PAVPSGTARS HDDEEGAGNP PIPEQDRPLL RVPREKEAKT GVSYFFP
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.
Gene References into Functions
  1. PKA inhibition by ARHGAP36 promotes derepression of the Hedgehog signalling pathway, thereby providing a simple rationale for the upregulation of ARHGAP36 in medulloblastoma. PMID: 27713425
  2. We have reported here for the first time a reduced activity of both Rac1 and Cdc42 in human pheochromocytoma resection as well as tumor-associated expression changes of FARP1, ARHGEF1, and ARHGAP36 PMID: 26911374
  3. a new mechanism of Gli transcription factor activation and implicate ARHGAP36 dysregulation in the onset and/or progression of GLI-dependent cancers. PMID: 25024229
  4. ARHGAP36 isoforms capable of Gli activation are up-regulated in a subset of human medulloblastomas. PMID: 25024229
Database Links

HGNC: 26388

OMIM: 300937

KEGG: hsa:158763

STRING: 9606.ENSP00000276211

UniGene: Hs.22905

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