Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
RHBDL2; Rhomboid-related protein 2; RRP2; EC 3.4.21.105; Rhomboid-like protein 2) [Cleaved into: Rhomboid-related protein 2; N-terminal fragment; NTF); Rhomboid-related protein 2; C-terminal fragment; CTF)]
Species
Homo sapiens (Human)
Expression Region
12-132aa
Target Protein Sequence
MNLNMGREMKEELEEEEKMREDGGGKDRAKSKKVHRIVSKWMLPEKSRGTYLERANCFPPPVFIISISLAELAVFIYYAVWKPQKQWITLDTGILESPFIYSPEKREEAWRFISYMLVHAG
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Amino acids 12-132 constitute the expression domain of recombinant Human RHBDL2. This RHBDL2 protein is theoretically predicted to have a molecular weight of 18.2 kDa. This protein is generated in a e.coli-based system. The RHBDL2 coding gene included the N-terminal 6xHis tag, which simplifies the detection and purification processes of the recombinant RHBDL2 protein in following stages of expression and purification.
Rhomboid-related protein 2 (RHBDL2) is a member of the rhomboid protease family, which is involved in intramembrane proteolysis. RHBDL2 has been implicated in various cellular processes, including the regulation of cell adhesion, migration, and inflammation. Additionally, some studies suggest its potential role in the cleavage of certain transmembrane proteins, contributing to the diversity of its cellular functions. Research on RHBDL2 may contribute to a better understanding of its substrate specificity, the cellular pathways it regulates, and its potential implications in cellular physiology and disease processes.
