Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

RHBDL2

Uniprot No.

Q9NX52

Research Area

Others

Alternative Names

RHBDL2; Rhomboid-related protein 2; RRP2; EC 3.4.21.105; Rhomboid-like protein 2) [Cleaved into: Rhomboid-related protein 2; N-terminal fragment; NTF); Rhomboid-related protein 2; C-terminal fragment; CTF)]

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

12-132aa

Target Protein Sequence

MNLNMGREMKEELEEEEKMREDGGGKDRAKSKKVHRIVSKWMLPEKSRGTYLERANCFPPPVFIISISLAELAVFIYYAVWKPQKQWITLDTGILESPFIYSPEKREEAWRFISYMLVHAG
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

18.2kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Amino acids 12-132 constitute the expression domain of recombinant Human RHBDL2. This RHBDL2 protein is theoretically predicted to have a molecular weight of 18.2 kDa. This protein is generated in a e.coli-based system. The RHBDL2 coding gene included the N-terminal 6xHis tag, which simplifies the detection and purification processes of the recombinant RHBDL2 protein in following stages of expression and purification.

Rhomboid-related protein 2 (RHBDL2) is a member of the rhomboid protease family, which is involved in intramembrane proteolysis. RHBDL2 has been implicated in various cellular processes, including the regulation of cell adhesion, migration, and inflammation. Additionally, some studies suggest its potential role in the cleavage of certain transmembrane proteins, contributing to the diversity of its cellular functions. Research on RHBDL2 may contribute to a better understanding of its substrate specificity, the cellular pathways it regulates, and its potential implications in cellular physiology and disease processes.

Target Background

Function

Involved in regulated intramembrane proteolysis and the subsequent release of functional polypeptides from their membrane anchors. Known substrate: EFNB3.

Gene References into Functions

Thrombomodulin and RHBDL2 are upregulated in human HaCaT cells stimulated by scratch wounds; furthermore, increased solulbe thrombomodulin was found in culture medium. PMID: 21833011
RHBDL2 cleaves epidermal growth factor just outside its transmembrane domain, thereby facilitating its secretion and triggering activation of the epidermal growth factor receptor. PMID: 21494248
Substrate specificity of RHBDL2 intramembrane protease is governed by helix-breaking residues in the transmembrane domain. PMID: 12820957
Here, the authors show that RHBDL2 is produced as a proenzyme and that the processing of RHBDL2 is required for its cellular protease activity. PMID: 19850051
The encoded protein is thought to release soluble growth factors by proteolytic cleavage of certain membrane-bound substrates, including ephrin B2 and ephrin B3. PMID: 15047175

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Subcellular Location

[Rhomboid-related protein 2, C-terminal fragment]: Cell membrane; Multi-pass membrane protein.

Protein Families

Peptidase S54 family

Database Links

HGNC: 16083

OMIM: 608962

KEGG: hsa:54933

STRING: 9606.ENSP00000289248

UniGene: Hs.524626

Code	CSB-EP019672HU
Abbreviation	Recombinant Human RHBDL2 protein, partial
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP019672HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) RHBDL2. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP019672HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) RHBDL2.
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Recombinant Human Rhomboid-related protein 2 (RHBDL2), partial

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