Recombinant Human Rhophilin-2 (RHPN2)

Code CSB-YP815544HU
MSDS
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Source Yeast
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Code CSB-EP815544HU
MSDS
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Source E.coli
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Code CSB-EP815544HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP815544HU
MSDS
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Source Baculovirus
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Code CSB-MP815544HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RHPN2
Uniprot No.
Alternative Names
76 kDa RhoB effector protein; D7Ertd784e; GTP Rho binding protein 2; GTP-Rho-binding protein 2; p76RBE; Rho GTPase binding protein 2; RhoB effector; RhoBP; Rhophilin 2; Rhophilin; Rhophilin like protein; Rhophilin like Rho GTPase binding protein ; Rhophilin Rho GTPase binding protein 2; Rhophilin-2; Rhophilin2; RHPN 2; RHPN2; RHPN2_HUMAN
Species
Homo sapiens (Human)
Expression Region
1-686
Target Protein Sequence
MTDALLPAAP QPLEKENDGY FRKGCNPLAQ TGRSKLQNQR AALNQQILKA VRMRTGAENL LKVATNSKVR EQVRLELSFV NSDLQMLKEE LEGLNISVGV YQNTEEAFTI PLIPLGLKET KDVDFAVVLK DFILEHYSED GYLYEDEIAD LMDLRQACRT PSRDEAGVEL LMTYFIQLGF VESRFFPPTR QMGLLFTWYD SLTGVPVSQQ NLLLEKASVL FNTGALYTQI GTRCDRQTQA GLESAIDAFQ RAAGVLNYLK DTFTHTPSYD MSPAMLSVLV KMMLAQAQES VFEKISLPGI RNEFFMLVKV AQEAAKVGEV YQQLHAAMSQ APVKENIPYS WASLACVKAH HYAALAHYFT AILLIDHQVK PGTDLDHQEK CLSQLYDHMP EGLTPLATLK NDQQRRQLGK SHLRRAMAHH EESVREASLC KKLRSIEVLQ KVLCAAQERS RLTYAQHQEE DDLLNLIDAP SVVAKTEQEV DIILPQFSKL TVTDFFQKLG PLSVFSANKR WTPPRSIRFT AEEGDLGFTL RGNAPVQVHF LDPYCSASVA GAREGDYIVS IQLVDCKWLT LSEVMKLLKS FGEDEIEMKV VSLLDSTSSM HNKSATYSVG MQKTYSMICL AIDDDDKTDK TKKISKKLSF LSWGTNKNRQ KSASTLCLPS VGAARPQVKK KLPSPFSLLN SDSSWY
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Binds specifically to GTP-Rho. May function in a Rho pathway to limit stress fiber formation and/or increase the turnover of F-actin structures in the absence of high levels of RhoA activity.
Gene References into Functions
  1. We found significant association between RHPN2 gene rs10411210 and colorectal cancer in European and Asian populations, but not American population PMID: 26349980
  2. Single nucleotide polymorphism in RHPN2 gene is associated with colorectal cancer. PMID: 25799222
  3. These effects were implemented through RHPN2-mediated activation of RhoA. PMID: 23774217
  4. Rhophilin-2 may function normally in a Rho pathway to limit stress fiber formation and/or increase the turnover of F-actin structures in the absence of high levels of RhoA activity PMID: 12221077
  5. may play a key role between RhoB and potential downstream elements needed under stimulation of the thyrotropin/cAMP pathway in thyrocytes PMID: 12473120

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Subcellular Location
Cytoplasm, perinuclear region.
Protein Families
RHPN family
Tissue Specificity
Widely expressed. Highly expressed in prostate, trachea, stomach, colon, thyroid and pancreas. Expressed at lower level in brain, spinal cord, kidney, placenta and liver.
Database Links

HGNC: 19974

OMIM: 617932

KEGG: hsa:85415

STRING: 9606.ENSP00000254260

UniGene: Hs.466435

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