Recombinant Human Riboflavin transporter 1 (GPR172B), partial

Code CSB-YP878904HU
MSDS
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Source Yeast
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Code CSB-EP878904HU
MSDS
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Source E.coli
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Code CSB-EP878904HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP878904HU
MSDS
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Source Baculovirus
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Code CSB-MP878904HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SLC52A1
Uniprot No.
Alternative Names
FLJ10060; G protein coupled receptor 172B; GPCR; GPCR42; hRFT1; member 1; OTTHUMP00000125300; PAR2; PERV-A receptor 2; PERVA receptor 2; Porcine endogenous retrovirus A receptor 2; Protein GPR172B; Putative G protein coupled receptor GPCR42; Riboflavin transporter 1; riboflavin transporter; S52A1_HUMAN; SLC52A1; Solute carrier family 52; Solute carrier family 52, riboflavin transporter, member 1
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Plasma membrane transporter mediating the uptake by cells of the water soluble vitamin B2/riboflavin that plays a key role in biochemical oxidation-reduction reactions of the carbohydrate, lipid, and amino acid metabolism. Humans are unable to synthesize vitamin B2/riboflavin and must obtain it via intestinal absorption.; (Microbial infection) May function as a cell receptor to retroviral envelopes similar to the porcine endogenous retrovirus (PERV-A).
Gene References into Functions
  1. We here report a case of transient MADD, caused by a heterozygous intronic variation, c.1134+11G>A, in the SLC52A1 gene encoding RFVT1. This variation creates a binding site for the splice inhibitory hnRNP A1 protein and causes exon 4 skipping. Riboflavin deficiency and maternal malnutrition during pregnancy might have been the determining factor in the outcome of this case. PMID: 29122468
  2. In HT-29 cells, the RFVT1 protein level was drastically lower. In tumor tissues of patients with CRC, RFVT1 content was reduced at both protein and mRNA levels compared to normal mucosa. PMID: 29715086
  3. results are the first to reveal the identity of the minimal SLC52A1 promoter and to establish an important role for Sp-1 in its activity PMID: 25284511
  4. data suggest that MMND is a distinct clinical subgroup of childhood onset MND patients where the known genetic defects are so far negative. PMID: 24139842
  5. summary of recent findings on the cloning, nomenclature, functional characterization and genetic diseases of RFVT1/SLC52A1, RFVT2/SLC52A2 and RFVT3/SLC52A3 [review] PMID: 23506902
  6. Intestinal riboflavin uptake process undergoes differentiation-dependent upregulation and suggest that this is mediated (at least in part) via transcriptional mechanisms of SLC52A1 and SLC52A3. PMID: 23413253
  7. We demonstrated that TFAP-2gamma is one of the transcription factors involved in the PAR-2 expression in human villous trophoblast cells. PMID: 22702469
  8. Identification/characterization riboflavin trasporter (RFT1) as a novel riboflavin transporter. PMID: 18632736

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Involvement in disease
Riboflavin deficiency (RBFVD)
Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
Riboflavin transporter family
Tissue Specificity
Widely expressed. Highly expressed in the testis, placenta and small intestine. Expressed at lower level in other tissues.
Database Links

HGNC: 30225

OMIM: 607883

KEGG: hsa:55065

STRING: 9606.ENSP00000254853

UniGene: Hs.632247

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