Recombinant Human Ribonuclease P protein subunit p29 (POP4)

Code CSB-YP018373HU
MSDS
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Source Yeast
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Code CSB-EP018373HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP018373HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP018373HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
POP4
Uniprot No.
Alternative Names
POP4; RPP29; Ribonuclease P protein subunit p29; hPOP4; EC 3.1.26.5
Species
Homo sapiens (Human)
Expression Region
1-220
Target Protein Sequence
MKSVIYHALS QKEANDSDVQ PSGAQRAEAF VRAFLKRSTP RMSPQAREDQ LQRKAVVLEY FTRHKRKEKK KKAKGLSARQ RRELRLFDIK PEQQRYSLFL PLHELWKQYI RDLCSGLKPD TQPQMIQAKL LKADLHGAII SVTKSKCPSY VGITGILLQE TKHIFKIITK EDRLKVIPKL NCVFTVETDG FISYIYGSKF QLRSSERSAK KFKAKGTIDL
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of ribonuclease P, a ribonucleoprotein complex that generates mature tRNA molecules by cleaving their 5'-ends.
Gene References into Functions
  1. Results demonstrate that Rpp29 promotes homology-directed repair of double-strand breaks. It is rapidly and transiently recruited to laser-microirradiated DNA sites. PMID: 28432356
  2. Collectively, the present results suggest that PhoRpp21 binds the loop between P11 and P12 helices through overall positively charged clusters on the surface of the complex and serves as a scaffold for PhoRpp29 to optimize structural conformation of its N-terminal helix (alpha2) in PhoRpp21, as well as C-terminal residues in PhoRpp29, for RNase P activity. PMID: 27810361
  3. Rpp29 represses H3.3 nucleosome deposition, a finding with implications for epigenetic regulation. PMID: 26842893
Subcellular Location
Nucleus, nucleolus.
Protein Families
Eukaryotic/archaeal RNase P protein component 1 family
Database Links

HGNC: 30081

OMIM: 606114

KEGG: hsa:10775

STRING: 9606.ENSP00000465213

UniGene: Hs.412870

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