Recombinant Human SAM domain-containing protein SAMSN-1 (SAMSN1)

Code CSB-YP885722HU
MSDS
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Source Yeast
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Code CSB-EP885722HU
MSDS
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Source E.coli
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Code CSB-EP885722HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP885722HU
MSDS
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Source Baculovirus
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Code CSB-MP885722HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SAMSN1
Uniprot No.
Alternative Names
HACS1; Hematopoietic adapter containing SH3 and sterile α, motif (SAM) domains 1; Hematopoietic adapter containing SH3 and sterile alpha motif (SAM) domains 1; Hematopoietic adaptor containing SH3 and SAM domains 1; Nash1; Nuclear localization signals SAM and SH3 domain containing 1; SAM and SH3 domain containing 2; SAM domain; SAM domain containing protein SAMSN 1; SAM domain SH3 domain and nuclear localisation signals 1; SAM domain SH3 domain and nuclear localization signals 1; SAM domain, SH3 domain and nuclear localization signals protein 1; SAM domain-containing protein SAMSN-1; SAMN1_HUMAN; SAMSN 1; Samsn1; SASH2; SH3 domain and nuclear localisation signals 1; SH3 domain and nuclear localization signals protein 1; SH3 SAM adaptor protein; SH3-SAM adaptor protein; SH3D6B; SLy2; Src homology domain 3 (SH3) containing adapter protein SH3 lymphocyte protein 2
Species
Homo sapiens (Human)
Expression Region
1-373
Target Protein Sequence
MLKRKPSNVS EKEKHQKPKR SSSFGNFDRF RNNSLSKPDD STEAHEGDPT NGSGEQSKTS NNGGGLGKKM RAISWTMKKK VGKKYIKALS EEKDEEDGEN AHPYRNSDPV IGTHTEKVSL KASDSMDSLY SGQSSSSGIT SCSDGTSNRD SFRLDDDGPY SGPFCGRARV HTDFTPSPYD TDSLKIKKGD IIDIICKTPM GMWTGMLNNK VGNFKFIYVD VISEEEAAPK KIKANRRSNS KKSKTLQEFL ERIHLQEYTS TLLLNGYETL EDLKDIKESH LIELNIENPD DRRRLLSAAE NFLEEEIIQE QENEPEPLSL SSDISLNKSQ LDDCPRDSGC YISSGNSDNG KEDLESENLS DMVHKIIITE PSD
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Negative regulator of B-cell activation. Down-regulates cell proliferation (in vitro). Promotes RAC1-dependent membrane ruffle formation and reorganization of the actin cytoskeleton. Regulates cell spreading and cell polarization. Stimulates HDAC1 activity. Regulates LYN activity by modulating its tyrosine phosphorylation.
Gene References into Functions
  1. Suppression of SAMSN1 Expression is Associated with the Malignant Phenotype of Hepatocellular Carcinoma. PMID: 25805236
  2. This is the first study to identify deletion of a key gene in the C57BL/KaLwRij mice that also displays reduced gene expression in patients with MM and is therefore likely to play an integral role in MM disease development. PMID: 25117979
  3. High expression of SAMSN1 is a significant risk factor for the progression free and overall survival of glioblastoma multiforme PMID: 24278465
  4. the immunoinhibitory roles of SLy2 in vivo and suggest that the physiological up-regulation of SLy2 observed upon B cell activation functions to counteract excessive B cell spreading. PMID: 21296879
  5. In the nucleus, SLy2 interacts with the SAP30/HDAC1 complex and regulates the activity of HDAC1. PMID: 20478393
  6. HACS1 is up-regulated by B cell activation signals and is a participant in B cell activation and differentiation. PMID: 15381729
  7. downregulation of SAMSN1 gene in human lung cancer. PMID: 18523997

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Subcellular Location
Nucleus. Cytoplasm. Cell projection, ruffle.
Tissue Specificity
Detected in peripheral blood B-cells (at protein level). Detected in spleen, liver and peripheral blood.
Database Links

HGNC: 10528

OMIM: 607978

KEGG: hsa:64092

STRING: 9606.ENSP00000383411

UniGene: Hs.473341

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