Recombinant Human SARS coronavirus Replicase polyprotein 1ab(rep), partial,Yeast

Code CSB-YP314477HQE
Size
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Product Details

Purity >85% (SDS-PAGE)
Target Names rep
Uniprot No. P0C6X7
Alternative Names rep; 1a-1b; Replicase polyprotein 1ab; pp1ab; ORF1ab polyprotein) [Cleaved into: Host translation inhibitor nsp1; nsp1; Leader protein); Non-structural protein 2; nsp2; p65 homolog); Papain-like proteinase; PL-PRO; EC 3.4.19.12; EC 3.4.22.69; Non-structural protein 3; nsp3; PL2-PRO; SARS coronavirus main proteinase); Non-structural protein 4; nsp4); 3C-like proteinase; 3CL-PRO; 3CLp; EC 3.4.22.-; nsp5); Non-structural protein 6; nsp6); Non-structural protein 7; nsp7); Non-structural protein 8; nsp8); Non-structural protein 9; nsp9); Non-structural protein 10; nsp10; Growth factor-like peptide; GFL); RNA-directed RNA polymerase; Pol; RdRp; EC 2.7.7.48; nsp12); Helicase; Hel; EC 3.6.4.12; EC 3.6.4.13; nsp13); Guanine-N7 methyltransferase; ExoN; EC 2.1.1.-; EC 3.1.13.-; nsp14); Uridylate-specific endoribonuclease; EC 3.1.-.-; NendoU; nsp15); 2'-O-methyltransferase; EC 2.1.1.-; nsp16)]
Species Human SARS coronavirus (SARS-CoV) (Severe acute respiratory syndrome coronavirus)
Source Yeast
Protein Length partial, Nsp13 function region
Tag Info N-terminal His-tagged
Tag-Free
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

Function
(From Uniprot)
Replicase polyprotein 1ab
Subcellular Location Papain-like proteinase: Host membrane, Multi-pass membrane protein, Host cytoplasm, SUBCELLULAR LOCATION: Non-structural protein 4: Host membrane, Multi-pass membrane protein, Host cytoplasm, Note=Localizes in virally-induced cytoplasmic double-membrane vesicles, SUBCELLULAR LOCATION: Non-structural protein 6: Host membrane, Multi-pass membrane protein, SUBCELLULAR LOCATION: Non-structural protein 7: Host cytoplasm, host perinuclear region, Note=nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear, Late in infection, they merge into confluent complexes (By similarity), SUBCELLULAR LOCATION: Non-structural protein 8: Host cytoplasm, host perinuclear region, Note=nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear, Late in infection, they merge into confluent complexes (By similarity), SUBCELLULAR LOCATION: Non-structural protein 9: Host cytoplasm, host perinuclear region, Note=nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear, Late in infection, they merge into confluent complexes (By similarity), SUBCELLULAR LOCATION: Non-structural protein 10: Host cytoplasm, host perinuclear region, Note=nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear, Late in infection, they merge into confluent complexes (By similarity), SUBCELLULAR LOCATION: Helicase: Host endoplasmic reticulum-Golgi intermediate compartment
Protein Families Coronaviruses polyprotein 1ab family
Database Links

KEGG: vg:1489680

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