Recombinant Human SEC14 domain and spectrin repeat-containing protein 1 (SESTD1)

Code CSB-YP801241HU
MSDS
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Source Yeast
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Code CSB-EP801241HU
MSDS
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Source E.coli
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Code CSB-EP801241HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP801241HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP801241HU
MSDS
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Source Mammalian cell
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Product Details

Purity
≥85% (SDS-PAGE)
Target Names
SESTD1
Uniprot No.
Alternative Names
huntingtin interacting protein like protein; Huntingtin-interacting protein-like protein; Protein Solo; SEC14 and spectrin domains 1; SEC14 domain and spectrin repeat-containing protein 1; SESD1_HUMAN; sestd1; SOLO
Species
Homo sapiens (Human)
Expression Region
1-696
Target Protein Sequence
MEASVILPIL KKKLAFLSGG KDRRSGLILT IPLCLEQTNM DELSVTLDYL LSIPSEKCKA RGFTVIVDGR KSQWNVVKTV VVMLQNVVPA EVSLVCVVKP DEFWDKKVTH FCFWKEKDRL GFEVILVSAN KLTRYIEPCQ LTEDFGGSLT YDHMDWLNKR LVFEKFTKES TSLLDELALI NNGSDKGNQQ EKERSVDLNF LPSVDPETVL QTGHELLSEL QQRRFNGSDG GVSWSPMDDE LLAQPQVMKL LDSLREQYTR YQEVCRQRSK RTQLEEIQQK VMQVVNWLEG PGSEQLRAQW GIGDSIRASQ ALQQKHEEIE SQHSEWFAVY VELNQQIAAL LNAGDEEDLV ELKSLQQQLS DVCYRQASQL EFRQNLLQAA LEFHGVAQDL SQQLDGLLGM LCVDVAPADG ASIQQTLKLL EEKLKSVDVG LQGLREKGQG LLDQISNQAS WAYGKDVTIE NKENVDHIQG VMEDMQLRKQ RCEDMVDVRR LKMLQMVQLF KCEEDAAQAV EWLSELLDAL LKTHIRLGDD AQETKVLLEK HRKFVDVAQS TYDYGRQLLQ ATVVLCQSLR CTSRSSGDTL PRLNRVWKQF TIASEERVHR LEMAIAFHSN AEKILQDCPE EPEAINDEEQ FDEIEAVGKS LLDRLTVPVV YPDGTEQYFG SPSDMASTAE NIRDRMKLVN LKRQQLRHPE MVTTES
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
May act as the primary docking protein directing membrane turnover and assembly of the transient receptor potential channels TRPC4 and TRPC5. Binds phospholipids such as phosphatidylinositol monophosphates, phosphatidylinositol diphosphates (PIP2s) and phosphatidic acid, but not less polar lipids including phosphatidylcholine, phosphatidylserine, and phosphatidylinositol. The binding to PIP2s is calcium dependent. Might be involved in the plasma membrane localization of CTNNB1.
Gene References into Functions
  1. Solo plays a crucial role in HD formation and acinar development in epithelial cells by regulating mechanical force-induced RhoA activation and keratin filament organization. PMID: 29672603
  2. The interplay between Solo protein and keratin 8/keratin 18 filaments plays a crucial role in tensile force-induced RhoA activation and consequent actin cytoskeletal reinforcement in endothelial cells. PMID: 26823019
  3. Upregulation of miR-532-5p and subsequent suppression of the SESTD1 and TAB3 genes represent an antiviral response aimed at limiting West Nile virus infection. PMID: 26676784
  4. SESTD1 was found to associate with TRPC4 and TRPC5 via the channel's calmodulin- and inositol 1,4,5-trisphosphate receptor-binding domain. PMID: 20164195
Protein Families
SOLO family
Tissue Specificity
Broad expression. High expression in thalamus and brain. Significantly expressed in vasculature.
Database Links

HGNC: 18379

KEGG: hsa:91404

STRING: 9606.ENSP00000415332

UniGene: Hs.30977

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China
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