Purity
Greater than 85% as determined by SDS-PAGE.
Research Area
Cell Biology
Alternative Names
DKFZP586F1318; RAY; SH3 domain containing; Ysc84-like 1 (S. cerevisiae); SH3 domain-containing YSC84-like protein 1; SH3Y1_HUMAN; Sh3yl1
Species
Homo sapiens (Human)
Expression Region
1-342aa
Target Protein Sequence
MNNPIPSNLKSEAKKAAKILREFTEITSRNGPDKIIPAHVIAKAKGLAILSVIKAGFLVTARGGSGIVVARLPDGKWSAPSAIGIAGLGGGFEIGIEVSDLVIILNYDRAVEAFAKGGNLTLGGNLTVAVGPLGRNLEGNVALRSSAAVFTYCKSRGLFAGVSLEGSCLIERKETNRKFYCQDIRAYDILFGDTPRPAQAEDLYEILDSFTEKYENEGQRINARKAAREQRKSSAKELPPKPLSRPQQSSAPVQLNSGSQSNRNEYKLYPGLSSYHERVGNLNQPIEVTALYSFEGQQPGDLNFQAGDRITVISKTDSHFDWWEGKLRGQTGIFPANYVTMN
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The recombinant Human SH3YL1 was expressed with the amino acid range of 1-342. The calculated molecular weight for this SH3YL1 protein is 41.1 kDa. The SH3YL1 protein was expressed in e.coli. The N-terminal 6xHis tag was fused into the coding gene segment of SH3YL1, making it easier to detect and purify the SH3YL1 recombinant protein in the later stages of expression and purification.
Human SH3 domain-containing YSC84-like protein 1 (SH3YL1) functions as a crucial mediator in cellular signaling, primarily regulating cell growth and differentiation. SH3YL1 is involved in modulating intracellular communication, influencing various cellular processes essential for maintaining proper cell function. Its significance extends to diverse research areas, including cancer, where it may serve as a potential biomarker or therapeutic target, and neurodegenerative disorders, where its role in neuronal function is explored. In regenerative medicine, SH3YL1's involvement in tissue regeneration and wound healing processes is investigated. Furthermore, in drug development, SH3YL1 modulation could be explored for identifying novel drug candidates.
